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nrdA recombinant protein :: Ribonucleoside-diphosphate reductase 1 subunit alpha Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS1264323
SDS-PAGE
Unit / Price
0.01 mg (E-Coli)  /  $110 +1 FREE 8GB USB
0.05 mg (E-Coli)  /  $190 +1 FREE 8GB USB
0.1 mg (E-Coli)  /  $285 +1 FREE 8GB USB
0.2 mg (E-Coli)  /  $460 +1 FREE 8GB USB
0.5 mg (E-Coli)  /  $750 +1 FREE 8GB USB
1 mg (E-Coli)  /  $1,180 +1 FREE 8GB USB
0.05 mg (Yeast)  /  $1,215 +1 FREE 8GB USB
0.05 mg (Baculovirus)  /  $1,330 +1 FREE 8GB USB
0.05 mg (Mammalian-Cell)  /  $1,585 +1 FREE 8GB USB
0.2 mg (Yeast)  /  $1,650 +2 FREE 8GB USB
0.1 mg (Baculovirus)  /  $1,690 +2 FREE 8GB USB
0.5 mg (Yeast)  /  $1,870 +2 FREE 8GB USB
0.1 mg (Mammalian-Cell)  /  $2,375 +2 FREE 8GB USB
0.5 mg (Baculovirus)  /  $2,410 +3 FREE 8GB USB
1 mg (Yeast)  /  $2,825 +3 FREE 8GB USB
1 mg (Baculovirus)  /  $3,150 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Ribonucleoside-diphosphate reductase 1 subunit alpha (nrdA), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Escherichia coli Ribonucleoside-diphosphate reductase 1 subunit alpha

 Product Synonym Names    Protein B1; Ribonucleoside-diphosphate reductase 1 R1 subunit; Ribonucleotide reductase 1
 Product Gene Name   

nrdA recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence Positions    1-761aa; Full Length
 Sequence    MNQNLLVTKR DGSTERINLD KIHRVLDWAA EGLHNVSISQ VELRSHIQFY DGIKTSDIHE TIIKAAADLI SRDAPDYQYL AARLAIFHLR KKAYGQFEPP ALYDHVVKMV EMGKYDNHLL EDYTEEEFKQ MDTFIDHDRD MTFSYAAVKQ LEGKYLVQNR VTGEIYESAQ FLYILVAACL FSNYPRETRL QYVKRFYDAV STFKISLPTP IMSGVRTPTR QFSSCVLIEC GDSLDSINAT SSAIVKYVSQ RAGIGINAGR IRALGSPIRG GEAFHTGCIP FYKHFQTAVK SCSQGGVRGG AATLFYPMWH LEVESLLVLK NNRGVEGNRV RHMDYGVQIN KLMYTRLLKG EDITLFSPSD VPGLYDAFFA DQEEFERLYT KYEKDDSIRK QRVKAVELFS LMMQERASTG RIYIQNVDHC NTHSPFDPAI APVRQSNLCL EIALPTKPLN DVNDENGEIA LCTLSAFNLG AINNLDELEE LAILAVRALD ALLDYQDYPI PAAKRGAMGR RTLGIGVINF AYYLAKHGKR YSDGSANNLT HKTFEAIQYY LLKASNELAK EQGACPWFNE TTYAKGILPI DTYKKDLDTI ANEPLHYDWE ALRESIKTHG LRNSTLSALM PSETSSQISN ATNGIEPPRG YVSIKASKDG ILRQVVPDYE HLHDAYELLW EMPGNDGYLQ LVGIMQKFID QSISANTNYD PSRFPSGKVP MQQLLKDLLT AYKFGVKTLY YQNTRDGAED AQDDLVPSIQ DDGCESGACK I
 3D Structure    ModBase 3D Structure for P00452
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 Host    E Coli or Yeast or Baculovirus or Mammalian Cell
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 Purity/Purification    Greater than 90% as determined by SDS-PAGE. (lot specific)
 Form/Format    Liquid containing glycerol
 Tag Information    This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
 Sterility    Sterile filter available upon request.
 Endotoxin    Low endotoxin available upon request.
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 Preparation and Storage    Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of nrdA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for nrdA recombinant protein

   Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R1 contains the binding sites for both substrates and allosteric effectors and carries out the actual reduction of the ribonucleotide. It also provides redox-active cysteines.
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 SDS-PAGE of nrdA recombinant protein    nrdA recombinant protein SDS-PAGE image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
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NCBI/Uniprot data below describe general gene information for nrdA. It may not necessarily be applicable to this product.
 NCBI GI #    16130169
 NCBI GeneID    946612
 NCBI Accession #    NP_416737.1 [Other Products]
 NCBI GenBank Nucleotide #    NC_000913.3 [Other Products]
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 UniProt Primary Accession #    P00452 [Other Products]
 UniProt Secondary Accession #    P78088; P78177 [Other Products]
 Molecular Weight    101.72kD
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 NCBI Official Full Name    ribonucleoside-diphosphate reductase 1, alpha subunit
 NCBI Official Symbol    nrdA [Similar Products]
 NCBI Official Synonym Symbols   
dnaF; ECK2226; JW2228
[Similar Products]
 NCBI Protein Information    ribonucleoside-diphosphate reductase 1, alpha subunit
 UniProt Protein Name    Ribonucleoside-diphosphate reductase 1 subunit alpha
 UniProt Synonym Protein Names   
Protein B1; Ribonucleoside-diphosphate reductase 1 R1 subunit; Ribonucleotide reductase 1
 Protein Family    Ribonucleoside-diphosphate reductase
 UniProt Gene Name    nrdA [Similar Products]
 UniProt Synonym Gene Names    dnaF [Similar Products]
 UniProt Entry Name    RIR1_ECOLI
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 NCBI Summary for nrdA    Reducing equivalents provided by glutaredoxin or thioredoxin. [More information is available at EcoGene: EG10660]. The B1 protein of ribonucleoside-diphosphate reductase consists of two polypeptide chains of similar or identical size. [More information is available at EcoCyc: EG10660].
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 UniProt Comments for nrdA    Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R1 contains the binding sites for both substrates and allosteric effectors and carries out the actual reduction of the ribonucleotide. It also provides redox-active cysteines.
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Product References and Citations for nrdA recombinant protein

   Primary structure of the Escherichia coli ribonucleoside diphosphate reductase operon.Carlson J., Fuchs J.A., Messing J.Proc. Natl. Acad. Sci. U.S.A. 81:4294-4297(1984) Nucleotide sequence of the gene coding for the large subunit of ribonucleotide reductase of Escherichia coli. Correction.Nilsson O., Aaberg A., Lundqvist T., Sjoeberg B.-M.Nucleic Acids Res. 16:4174-4174(1988) Sjoeberg B.-M.Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features.Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.DNA Res. 4:91-113(1997) The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) Protein B1 of ribonucleotide reductase. Direct analytical data and comparisons with data indirectly deduced from the nucleotide sequence of the Escherichia coli nrdA gene.Sjoeberg B.-M., Eriksson S., Joernvall H., Carlquist M., Eklund H.Eur. J. Biochem. 150:423-427(1985) Evidence for two different classes of redox-active cysteines in ribonucleotide reductase of Escherichia coli.Aaberg A., Hahne S., Karlsson M., Larsson A., Ormoe M., Aahgren A., Sjoeberg B.-M.J. Biol. Chem. 264:12249-12252(1989) Two conserved tyrosine residues in protein R1 participate in an intermolecular electron transfer in ribonucleotide reductase.Ekberg M., Sahlin M., Eriksson M., Sjoberg B.M.J. Biol. Chem. 271:20655-20659(1996) Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.Zhang J., Sprung R., Pei J., Tan X., Kim S., Zhu H., Liu C.F., Grishin N.V., Zhao Y.Mol. Cell. Proteomics 8:215-225(2009) Structure of ribonucleotide reductase protein R1.Uhlin U., Eklund H.Nature 370:533-539(1994) A new mechanism-based radical intermediate in a mutant R1 protein affecting the catalytically essential Glu441 in Escherichia coli ribonucleotide reductase.Persson A.L., Eriksson M., Katterle B., Potsch S., Sahlin M., Sjoberg B.M.J. Biol. Chem. 272:31533-31541(1997) Binding of allosteric effectors to ribonucleotide reductase protein R1 reduction of active-site cysteines promotes substrate binding.Eriksson M., Uhlin U., Ramaswamy S., Ekberg M., Regnstroem K., Sjoeberg B.-M., Eklund H.Structure 5:1077-1092(1997) Binding specificity and mechanistic insight into glutaredoxin-catalyzed protein disulfide reduction.Berardi M.J., Bushweller J.H.J. Mol. Biol. 292:151-161(1999)
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 Research Articles on nrdA    1. The present results identifying a large, new set of E. coli mutator strains with mutations of nrdAB ribonucleotide reductase(RNR)confirm the important control that RNR exerts on the cellular mutation rate and provide novel insights into the RNR feedback regulatory mechanisms.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with nrdA recombinant proteinPathways associated with nrdA recombinant protein
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 thyA recombinant protein  >1 publications with nrdA and thyA
 ndk recombinant protein  >1 publications with nrdA and ndk
 nrdH recombinant protein  >1 publications with nrdA and nrdH
 dut recombinant protein  >1 publications with nrdA and dut
 dnaN recombinant protein  >1 publications with nrdA and dnaN
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