NP_416738.1
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NCBI GenBank Nucleotide #
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UniProt Primary Accession #
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UniProt Secondary Accession #
Molecular Weight
45.4 kDa
NCBI Official Full Name
ribonucleoside-diphosphate reductase 1, beta subunit, ferritin-like protein
NCBI Protein Information
ribonucleoside-diphosphate reductase 1, beta subunit, ferritin-like protein
UniProt Protein Name
Ribonucleoside-diphosphate reductase 1 subunit beta
UniProt Synonym Protein Names
Protein B2; Protein R2; Ribonucleotide reductase 1
UniProt Synonym Gene Names
UniProt Entry Name
RIR2_ECOLI
NCBI Summary for nrdB
Reducing equivalents provided by glutaredoxin or thioredoxin. [More information is available at EcoGene: EG10661]. The B2 protein of ribonucleoside-diphosphate reductase contains the tyrosyl radical-dinuclear iron center, which is thought to initiate catalysis by long-range electron transfer. [More information is available at EcoCyc: EG10661].
UniProt Comments for nrdB
Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R2 contains the tyrosyl radical required for catalysis.
Product References and Citations for nrdB recombinant protein
Primary structure of the Escherichia coli ribonucleoside diphosphate reductase operon.Carlson J., Fuchs J.A., Messing J.Proc. Natl. Acad. Sci. U.S.A. 81:4294-4297(1984)
Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features.Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.DNA Res. 4:91-113(1997)
The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997)
Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006)
Cloning, overproduction, and purification of the B2 subunit of ribonucleoside-diphosphate reductase.Salowe S.P., Stubbe J.J. Bacteriol. 165:363-366(1986)
Three-dimensional structure of the free radical protein of ribonucleotide reductase.Nordlund P., Sjoeberg B.-M., Eklund H.Nature 345:593-598(1990)
Structure and function of the Escherichia coli ribonucleotide reductase protein R2.Nordlund P., Eklund H.J. Mol. Biol. 232:123-164(1993)
Crystal structure of reduced protein R2 of ribonucleotide reductase
the structural basis for oxygen activation at a dinuclear iron site.Logan D.T., Su X.-D., Aaberg A., Regnstroem K., Hajdu J., Eklund H., Nordlund P.Structure 4:1053-1064(1996)
Binding of allosteric effectors to ribonucleotide reductase protein R1
reduction of active-site cysteines promotes substrate binding.Eriksson M., Uhlin U., Ramaswamy S., Ekberg M., Regnstroem K., Sjoeberg B.-M., Eklund H.Structure 5:1077-1092(1997)
Characterization of Y122F R2 of Escherichia coli ribonucleotide reductase by time-resolved physical biochemical methods and X-ray crystallography.Tong W., Burdi D., Riggs-Gelasco P., Chen S., Edmondson D., Huynh B.H., Stubbe J., Han S., Arvai A., Tainer J.Biochemistry 37:5840-5848(1998)
Crystal structures of two self-hydroxylating ribonucleotide reductase protein R2 mutants
structural basis for the oxygen-insertion step of hydroxylation reactions catalyzed by diiron proteins.Logan D.T., De;Mare F., Persson B.O., Slaby A., Sjoeberg B.-M., Nordlund P.Biochemistry 37:10798-10807(1998)
Crystal structures of oxidized dinuclear manganese centres in Mn-substituted class I ribonucleotide reductase from Escherichia coli
carboxylate shifts with implications for O2 activation and radical generation.Hogbom M., Andersson M.E., Nordlund P.J. Biol. Inorg. Chem. 6:315-323(2001)
Research Articles on nrdB
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Products associated with nrdB recombinant protein
Pathways associated with nrdB recombinant protein
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