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S100a8 recombinant protein :: S100-A8 Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS952076
SDS-Page
Unit / Price
0.01 mg (E-Coli)  /  $110 +1 FREE 8GB USB
0.05 mg (E-Coli)  /  $190 +1 FREE 8GB USB
0.1 mg (E-Coli)  /  $285 +1 FREE 8GB USB
0.2 mg (E-Coli)  /  $460 +1 FREE 8GB USB
0.05 mg (Yeast)  /  $655 +1 FREE 8GB USB
0.5 mg (E-Coli)  /  $750 +1 FREE 8GB USB
0.05 mg (Baculovirus)  /  $810 +1 FREE 8GB USB
0.2 mg (Yeast)  /  $885 +1 FREE 8GB USB
0.5 mg (Yeast)  /  $990 +1 FREE 8GB USB
0.1 mg (Baculovirus)  /  $1,020 +1 FREE 8GB USB
0.05 mg (Mammalian-Cell)  /  $1,050 +1 FREE 8GB USB
1 mg (E-Coli)  /  $1,180 +1 FREE 8GB USB
0.5 mg (Baculovirus)  /  $1,480 +1 FREE 8GB USB
1 mg (Yeast)  /  $1,505 +1 FREE 8GB USB
0.1 mg (Mammalian-Cell)  /  $1,605 +2 FREE 8GB USB
1 mg (Baculovirus)  /  $1,990 +2 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

S100-A8 (S100a8), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Mouse Protein S100-A8

 Product Synonym Names    Calgranulin-A; Chemotactic cytokine CP-10; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8; MRP-8; p8; Pro-inflammatory S100 cytokine; S100 calcium-binding protein A8
 Product Gene Name   

S100a8 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence Positions    2-89aa; Full Length
 Sequence    PSELEKALSN LIDVYHNYSN IQGNHHALYK NDFKKMVTTE CPQFVQNINI ENLFRELDIN SDNAINFEEF LAMVIKVGVA SHKDSHKE
 3D Structure    ModBase 3D Structure for P27005
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 Host    E Coli or Yeast or Baculovirus or Mammalian Cell
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 Purity/Purification    Greater than 90% as determined by SDS-PAGE. (lot specific)
 Form/Format    Liquid containing glycerol
 Tag Information    This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
 Sterility    Sterile filter available upon request.
 Endotoxin    Low endotoxin available upon request.
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 Preparation and Storage    Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of S100a8 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for S100a8 recombinant protein

   S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn2+ which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. The iNOS-S100A8/A9 transnitrosylase complex is proposed to direct selective inflammatory stimulus-dependent S-nitrosylation of multiple targets such as GAPDH, ANXA5, EZR, MSN and VIM by recognizing a [IL]-x-C-x-x-[DE] motif; S100A8 ses to contribute to S-nitrosylation site selectivity.
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 SDS-Page of S100a8 recombinant protein    S100a8 recombinant protein SDS-Page image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
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NCBI/Uniprot data below describe general gene information for S100a8. It may not necessarily be applicable to this product.
 NCBI GI #    7305453
 NCBI GeneID    20201
 NCBI Accession #    NP_038678.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_013650.2 [Other Products]
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 UniProt Primary Accession #    P27005 [Other Products]
 UniProt Secondary Accession #    P31724 [Other Products]
 UniProt Related Accession #    P27005 [Other Products]
 Molecular Weight    26.16kD
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 NCBI Official Full Name    protein S100-A8
 NCBI Official Synonym Full Names    S100 calcium binding protein A8 (calgranulin A)
 NCBI Official Symbol    S100a8 [Similar Products]
 NCBI Official Synonym Symbols   
p8; B8Ag; CFAg; Caga; MRP8; CP-10; 60B8Ag; AI323541
[Similar Products]
 NCBI Protein Information    protein S100-A8
 UniProt Protein Name    Protein S100-A8
 UniProt Synonym Protein Names   
Calgranulin-A; Chemotactic cytokine CP-10; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8; MRP-8; p8; Pro-inflammatory S100 cytokine; S100 calcium-binding protein A8
 Protein Family    Protein
 UniProt Gene Name    S100a8 [Similar Products]
 UniProt Synonym Gene Names    Caga; Mrp8; MRP-8; p8 [Similar Products]
 UniProt Entry Name    S10A8_MOUSE
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 UniProt Comments for S100a8    S100A8: S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH- oxidase by facilitating the enzyme complex assembly at the cell membrane, transfering arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF- kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. Belongs to the S-100 family.

Cellular Component: cytoplasm; cytoskeleton; extracellular region; extracellular space; membrane; nucleus; plasma membrane

Molecular Function: antioxidant activity; arachidonic acid binding; calcium ion binding; metal ion binding; microtubule binding; RAGE receptor binding; zinc ion binding

Biological Process: apoptosis; astrocyte development; autophagy; caspase activation; chemotaxis; immune system process; inflammatory response; innate immune response; leukocyte migration during inflammatory response; neutrophil chemotaxis; peptidyl-cysteine S-nitrosylation; positive regulation of inflammatory response; positive regulation of peptide secretion; regulation of inflammatory response
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Product References and Citations for S100a8 recombinant protein

   Mouse MRP8 and MRP14, two intracellular calcium-binding proteins associated with the development of the myeloid lineage.Lagasse E., Weissman I.L.Blood 79:1907-1915(1992) Identification of a chemotactic domain of the pro-inflammatory S100 protein CP-10.Lackmann M., Rajasekariah P., Iismaa S.E., Jones G., Cornish C.J., Hu S., Simpson R.J., Moritz R.L., Geczy C.L.J. Immunol. 150:2981-2991(1993) Molecular characterisation of the genomic locus of the mouse MRP8 gene.Nacken W.K.F., Manitz M.P., Sorg C.Biochim. Biophys. Acta 1315:1-5(1996) Purification and structural analysis of a murine chemotactic cytokine (CP-10) with sequence homology to S100 proteins.Lackmann M., Cornish C.J., Simpson R.J., Moritz R.L., Geczy C.L.J. Biol. Chem. 267:7499-7504(1992) A null mutation in the inflammation-associated S100 protein S100A8 causes early resorption of the mouse embryo.Passey R.J., Williams E., Lichanska A.M., Wells C., Hu S., Geczy C.L., Little M.H., Hume D.A.J. Immunol. 163:2209-2216(1999) Mrp8 and Mrp14 are endogenous activators of Toll-like receptor 4, promoting lethal, endotoxin-induced shock.Vogl T., Tenbrock K., Ludwig S., Leukert N., Ehrhardt C., van Zoelen M.A.D., Nacken W., Foell D., van der Poll T., Sorg C., Roth J.Nat. Med. 13:1042-1049(2007) S100A8 and S100A9 mediate endotoxin-induced cardiomyocyte dysfunction via the receptor for advanced glycation end products.Boyd J.H., Kan B., Roberts H., Wang Y., Walley K.R.Circ. Res. 102:1239-1246(2008) S-nitrosylated S100A8 novel anti-inflammatory properties.Lim S.Y., Raftery M., Cai H., Hsu K., Yan W.X., Hseih H.L., Watts R.N., Richardson D., Thomas S., Perry M., Geczy C.L.J. Immunol. 181:5627-5636(2008) Anti-infective protective properties of S100 calgranulins.Hsu K., Champaiboon C., Guenther B.D., Sorenson B.S., Khammanivong A., Ross K.F., Geczy C.L., Herzberg M.C.Antiinflamm. Antiallergy Agents Med. Chem. 8:290-305(2009) Inflammation-associated S100 proteins new mechanisms that regulate function.Goyette J., Geczy C.L.Amino Acids 41:821-842(2011)
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 Research Articles on S100a8    1. Inflammation-induced S100A8 promotes colorectal tumorigenesis by acting upstream to activate the Akt1-Smad5-Id3 axis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with S100a8 recombinant proteinDiseases associated with S100a8 recombinant protein
 Reference Product  PubMed Publications
 S100a9 recombinant protein  >820 publications with S100a8 and S100a9
 Met recombinant protein  >11 publications with S100a8 and Met
 Camp recombinant protein  >6 publications with S100a8 and Camp
 Src recombinant protein  >6 publications with S100a8 and Src
 Bax recombinant protein  >4 publications with S100a8 and Bax
 Mark2 recombinant protein  >1 publications with S100a8 and Mark2
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >478 publications with S100a8 and Inflammation
 Disease Models, Animal Antibodies  >117 publications with S100a8 and Disease Models, Animal
 Fibrosis Antibodies  >110 publications with S100a8 and Fibrosis
 Necrosis Antibodies  >96 publications with S100a8 and Necrosis
 Cardiovascular Diseases Antibodies  >82 publications with S100a8 and Cardiovascular Diseases
 Nervous System Diseases Antibodies  >72 publications with S100a8 and Nervous System Diseases
 Atherosclerosis Antibodies  >50 publications with S100a8 and Atherosclerosis
 Brain Diseases Antibodies  >44 publications with S100a8 and Brain Diseases
 Heart Diseases Antibodies  >36 publications with S100a8 and Heart Diseases
 Kidney Diseases Antibodies  >34 publications with S100a8 and Kidney Diseases
Organs/Tissues associated with S100a8 recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >122 publications with S100a8 and Lung
 Joint Antibodies  >59 publications with S100a8 and Joint
 Brain Antibodies  >57 publications with S100a8 and Brain
 Bone Marrow Antibodies  >56 publications with S100a8 and Bone Marrow
 Kidney Antibodies  >54 publications with S100a8 and Kidney
 Liver Antibodies  >50 publications with S100a8 and Liver
 Lymph Node Antibodies  >31 publications with S100a8 and Lymph Node
 Eye Antibodies  >22 publications with S100a8 and Eye
 Spleen Antibodies  >20 publications with S100a8 and Spleen
 Embryonic Tissue Antibodies  >19 publications with S100a8 and Embryonic Tissue
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