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SCF recombinant protein

Scan QR to view Datasheet Catalog #    MBS691568
Unit / Price
0.01 mg  /  $255 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

SCF, Recombinant Protein

★Popular Item★
 Also Known As   

Mouse SCF

 Product Synonym Names    Recombinant Mouse Stem Cell Factor (SCF); Hematopoietic growth factor KL; c-Kit ligand
 Product Gene Name   

SCF recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQ LSLSLTTLLD KFSNISEGLS NYSIIDKLGK IVDDLVLCME ENAPKNIKES PK RPETRSFTPE EFFSIFNRSI DAFKDFMVAS DTSDCVLSST LGPEKDSRVS VT KPFMLPPVA
 Chromosome Location    Chromosome: 10; NC_000076.6 (100015787..100100416). Location: 10 D1; 10 51.4 cM
 3D Structure    ModBase 3D Structure for P20826
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 Host    E Coli
 Species Reactivity    Mouse
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 Purity/Purification    > 95% by SDS-PAGE & silver stain
 Form/Format    Lyophilized
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 Endotoxin Level    < 0.1ng/ug of mSCF
  Length (aa)    165
 Biological Activity    The ED50 as determined by the dose-depend stimulation of the proliferation of the human TF-1 cell line is in the range of 2-10 ng/ml.
 Buffer    0.5X PBS
 Gene    Kitlg
 Reconstitution    Mouse SCF should be reconstituted in 50mM acetis acid or water to a concentration of 0.1 mg/ml. This solution can be diluted in water or other buffer solutions or stored at -20 degree C.
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 Preparation and Storage    The lyophilized mouse SCF is best stored desiccated below 0 degree C. Freeze/thaw cycles will result in significant loss of activity. Avoid repeated freeze-thaw cycles.
 ISO Certification    Manufactured in an ISO 9001:2015 Certified Laboratory.
 Other Notes    Small volumes of SCF recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for SCF recombinant protein

   Stem cell factor (SCF), also known as ckit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28-40 kDa type I transmembrane glycoprotein. It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors. SCF is a primary growth and activation factor for mast cells and eosinophils. Mature mouse SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail. Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble. An alternately spliced isoform of mouse SCF lacks 28 aa that encompasses the primary proteolytic recognition site. Within the ECD of the short isoform, mouse SCF shares 93% aa sequence identity with rat SCF and 72%-75% with canine, feline, and human SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells. Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/ckit to trigger receptor dimerization and signaling.
 Product Categories/Family for SCF recombinant protein    Cytokines & Growth Factors
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 Testing Data #2 of SCF recombinant protein    SCF recombinant protein Testing Data #2 image
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 Test Data of SCF recombinant protein    Proliferation assay with TF-I cells.
SCF recombinant protein Test Data image
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NCBI/Uniprot data below describe general gene information for SCF. It may not necessarily be applicable to this product.
 NCBI GI #    7305267
 NCBI GeneID    17311
 NCBI Accession #    NP_038626.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_013598.2 [Other Products]
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 UniProt Primary Accession #    P20826 [Other Products]
 UniProt Secondary Accession #    P97332; Q3TNJ7; Q62524; Q64222; Q921N5 [Other Products]
 UniProt Related Accession #    P20826 [Other Products]
 Molecular Weight    18.42 kDa
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 NCBI Official Full Name    kit ligand
 NCBI Official Synonym Full Names    kit ligand
 NCBI Official Symbol    Kitl [Similar Products]
 NCBI Official Synonym Symbols   
Gb; SF; Sl; Clo; Con; Mgf; SCF; SLF; blz; Kitlg; contrasted
[Similar Products]
 NCBI Protein Information    kit ligand; cloud gray; C-kit ligand; Steel factor; grizzle-belly; stem cell factor; mast cell growth factor; hematopoietic growth factor KL
 UniProt Protein Name    Kit ligand
 UniProt Synonym Protein Names   
Hematopoietic growth factor KL; Mast cell growth factor; MGF; Steel factor; Stem cell factor; SCF; c-Kit ligand
 Protein Family    SCF-associated factor
 UniProt Gene Name    Kitlg [Similar Products]
 UniProt Synonym Gene Names    Kitl; Mgf; Sl; Slf; MGF; SCF; sKITLG [Similar Products]
 UniProt Entry Name    SCF_MOUSE
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 UniProt Comments for SCF    Function: Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.

Subunit structure: Homodimer, non-covalently linked

Probable. Heterotetramer with KIT, binding two KIT molecules; thereby mediates KIT dimerization and subsequent activation by autophosphorylation. Ref.14

Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein

By similarity. Isoform 2: Cell membrane; Single-pass type I membrane protein

By similarity. Cytoplasm › cytoskeleton

By similarity. Soluble KIT ligand: Secreted

By similarity.

Developmental stage: Acts in the early stages of hematopoiesis.

Post-translational modification: A soluble form is produced by proteolytic processing of isoform 1 in the extracellular domain.

Sequence similarities: Belongs to the SCF family.
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Product References and Citations for SCF recombinant protein

   1. Ashman LK Int J Biochem Cell Biol 31 (1999) 2. Sette et al, Int J Dev Biol 44 (2000) 5. Kapur et al, Blood 100 (2002) 6. Wang et al, Arterioscler Thromb Vasc Biol 27 (2007) 7. Bashamboo et al, J Cell Sci 119 (2006) 10. Arakawa et al, J Biol Chem 266 (1991) 13. Flanagan et al, Cell 64 (1991) 14. Martin et al, Cell 63 (1990) 15. Lemmon et al, J Biol Chem 272 (1997)
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 Research Articles on SCF    1. Wnt7a triggers melanocyte stem cell differentiation through beta-catenin activation, and Kitl might induce following migration of melanoblasts to epidermis
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with SCF recombinant proteinDiseases associated with SCF recombinant protein
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies  Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Downstream Signaling Events Of B Cell Receptor (BCR) Pathway antibodies  Downstream Signaling Events Of B Cell Receptor (BCR) Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram
 Downstream Signaling Of Activated FGFR Pathway antibodies  Downstream Signaling Of Activated FGFR Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >17 publications with SCF and Neoplasms
 Disease Models, Animal Antibodies  >9 publications with SCF and Disease Models, Animal
 Neoplasms, Experimental Antibodies  >5 publications with SCF and Neoplasms, Experimental
 Inflammation Antibodies  >5 publications with SCF and Inflammation
 Weight Loss Antibodies  >3 publications with SCF and Weight Loss
 Fibrosis Antibodies  >3 publications with SCF and Fibrosis
 Lung Diseases Antibodies  >3 publications with SCF and Lung Diseases
 Necrosis Antibodies  >3 publications with SCF and Necrosis
 Carcinoma Antibodies  >2 publications with SCF and Carcinoma
 Lung Neoplasms Antibodies  >1 publications with SCF and Lung Neoplasms
Organs/Tissues associated with SCF recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >30 publications with SCF and Bone
 Ovary Antibodies  >23 publications with SCF and Ovary
 Skin Antibodies  >20 publications with SCF and Skin
 Bone Marrow Antibodies  >19 publications with SCF and Bone Marrow
 Testis Antibodies  >17 publications with SCF and Testis
 Embryonic Tissue Antibodies  >9 publications with SCF and Embryonic Tissue
 Liver Antibodies  >7 publications with SCF and Liver
 Heart Antibodies  >5 publications with SCF and Heart
 Muscle Antibodies  >5 publications with SCF and Muscle
 Lung Antibodies  >5 publications with SCF and Lung
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