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SIRPA recombinant protein :: SIRP alpha Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS637456 SIRPA recombinant protein
Unit / Price
0.05 mg  /  $725 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

SIRP alpha (SIRPA), Recombinant Protein

 Also Known As   

SIRP alpha, Recombinant, Human (SIRPa, Signal Regulatory Protein, SHPS-1, BIT, p84, CD172a)

 Product Gene Name   

SIRPA recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 20; NC_000020.10 (1874763..1920540). Location: 20p13
 OMIM    602461
 3D Structure    ModBase 3D Structure for P78324
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 Host    Recombinant Human
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 Purity/Purification    Highly Purified
90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
 Form/Format    Supplied as a lyophilized powder in PBS, pH 7.4.
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 Activity    Measured by the ability of the immobilized protein to support the adhesion of red blood cells (Subramanian S., et al., 2006, Blood 107:2548-2556).
 Endotoxin Level    1EU/1ug.
 Reconstitute    Reconstitute with 500ul sterile PBS.
 N-terminal Sequence Analysis    Gly27 and Glu31
 SDS-PAGE    70-105kD, reducing conditions.
 Activity    Measured by the ability of the immobilzed protein to support the adhesion of red blood cells (Subramanian S., et al., 2006, Blood 107: 2548-2556). Red blood cells added at 10x 10e6 cells/ml (100ul/well) to SIRPa coated plated will adhere after 1 hour at 37 degree C. The ED50 for this effect is 0.6-2.4ug/ml
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 Preparation and Storage    Lyophilized powder may be stored at -20 degree C. Stable for 12 months after receipt at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
 Other Notes    Small volumes of SIRPA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for SIRPA recombinant protein

   Signal regulatory protein alpha (SIRPA, designated CD172a), also called SHPS-1 (SHP substrate 1) and previously, MyD-1 (Myeloid/Dendritic-1), is a monomeric ~90kD type I transmembrane glycoprotein that belongs to the SIRP/SHPS (CD172) family of the immunoglobulin superfamily.1-4 SIRPs are paired receptors, with similar extracellular domains but differing C-termini and functions.1, 2 The 503 amino acid (aa) human SIRPA contains a 342 aa extracellular domain (ECD), with one V-type, and two C1-type Ig domains, and three potential N-glycosylation sites. It has a 110 aa cytoplasmic sequence with ITIM motifs that recruit tyrosine phosphatases SHP-1 and SHP-2 when phosphorylated.4 Human SIRPA has more than 40 described polymorphisms, including the prominent BIT (Brain Ig-like molecule with Tyrosine-based activation motifs, also called SIRPA2 or PTPNS).5 One reported isoform lacks aa 1-101, which eliminates most of the V-type Ig domain. Human SIRPA ECD shares 61%, 60%, 71%, 72% and 73% aa identity with mouse, rat, porcine, bovine and equine SIRPA, respectively; it shares 84% and 76% aa identity with human SIRPB1 and SIRPy, respectively.2 SIRPA is expressed mainly on myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells.3-6 It is also found on neurons, smooth muscle and endothelial cells.7-9 SIRPA shows adhesion to the ubiquitous CD47/IAP (integrin-associated protein), while SIRPY binds more weakly and SIRPA1 does not bind at all.1, 2 Mouse and human SIRPA-CD47 binding only cross-reacts for specific polymorphisms and influences engraftment of xenotransplanted stem cells.6, 10 SIRPA engagement generally produces a negative regulatory signal.4 Low SIRPA recognition of CD47, which occurs on aged erythrocytes or platelets or xenogenic cells, promotes clearance of CD47low cells from circulation.11-13 SIRPA Arecognition of surfactants SP-A and SP-D in the lung can inhibit alveolar macrophage cytokine production.14 CD47- integrin-SIRPA interaction is reported to promote macrophage fusion during osteoclastogenesis.15

A DNA sequence encoding the extracellular domain of human SIRP (Gly 27-Arg 370; Accession # NP_542970) was fused to the Fc region of human IgG1 via a polypeptide linker. The chimeric protein was expressed in Chinese hamster ovary cells.
Calculated molecular mass of ~ 64kD. As a result of glycosylation, the recombinant protein migrates as an ~ 70-105kD protein in SDS-PAGE under reducing conditions.
 Product Categories/Family for SIRPA recombinant protein    Molecular Biology; MB-Protein Kinase Substrates
NCBI/Uniprot data below describe general gene information for SIRPA. It may not necessarily be applicable to this product.
 NCBI GI #    49899261
 NCBI GeneID    140885
 NCBI Accession #    AAH75849.1 [Other Products]
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 UniProt Primary Accession #    P78324 [Other Products]
 UniProt Secondary Accession #    O00683; O43799; Q8N517; Q8TAL8; Q9H0Z2; Q9UDX2; Q9UIJ6; Q9Y4U9; A2A2E1; A8K411; B2R6C3 [Other Products]
 UniProt Related Accession #    P78324 [Other Products]
 Molecular Weight    54,967 Da [Similar Products]
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 NCBI Official Full Name    Signal-regulatory protein alpha
 NCBI Official Synonym Full Names    signal-regulatory protein alpha
 NCBI Official Symbol    SIRPA [Similar Products]
 NCBI Official Synonym Symbols   
BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1
[Similar Products]
 NCBI Protein Information    tyrosine-protein phosphatase non-receptor type substrate 1; myd-1 antigen; inhibitory receptor SHPS-1; macrophage fusion receptor; CD172 antigen-like family member A; tyrosine phosphatase SHP substrate 1; brain-immunoglobulin-like molecule with tyrosine-based activation motifs
 UniProt Protein Name    Tyrosine-protein phosphatase non-receptor type substrate 1
 UniProt Synonym Protein Names   
Brain Ig-like molecule with tyrosine-based activation motifs; Bit; CD172 antigen-like family member A; Inhibitory receptor SHPS-1; Macrophage fusion receptor; MyD-1 antigen; Signal-regulatory protein alpha-1; Sirp-alpha-1; Signal-regulatory protein alpha-2; Sirp-alpha-2; Signal-regulatory protein alpha-3; Sirp-alpha-3; p84
 Protein Family    Tyrosine-protein phosphatase non-receptor
 UniProt Gene Name    SIRPA [Similar Products]
 UniProt Synonym Gene Names    BIT; MFR; MYD1; PTPNS1; SHPS1; SIRP; SHP substrate 1; SHPS-1; Bit; Sirp-alpha-1; Sirp-alpha-2 [Similar Products]
 UniProt Entry Name    SHPS1_HUMAN
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 NCBI Summary for SIRPA    The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for SIRPA    SHPS1: Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Binds PTPN11 when tyrosine-phosphorylated, except in macrophages, where it primarily binds PTPN6. Binds GRB2 in vitro. Binds FGR. Binds JAK2 irrespective of its phosphorylation status and forms a stable complex. Binds SCAP1 and/or SCAP2. The resulting complex recruits FYB. Binds PTK2B. Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Cell adhesion; Membrane protein, integral; Cell surface; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: membrane; plasma membrane; integral to membrane

Molecular Function: SH3 domain binding

Biological Process: cell adhesion; blood coagulation; leukocyte migration
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 Research Articles on SIRPA    1. Expression of SIRPalpha on two low SIRPalpha acute myeloid leukemia (AML) expressing cell lines could be upregulated upon differentiation of the cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with SIRPA recombinant proteinDiseases associated with SIRPA recombinant protein
 Products by Pathway  Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Cardiac Progenitor Differentiation Pathway antibodies  Cardiac Progenitor Differentiation Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Cell-Cell Communication Pathway antibodies  Cell-Cell Communication Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 IL-1 Signaling Pathway antibodies  IL-1 Signaling Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
 Prolactin Signaling Pathway antibodies  Prolactin Signaling Pathway Diagram
 Signal Regulatory Protein (SIRP) Family Interactions Pathway antibodies  Signal Regulatory Protein (SIRP) Family Interactions Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >39 publications with SIRPA and Neoplasms
 Inflammation Antibodies  >16 publications with SIRPA and Inflammation
 Nervous System Diseases Antibodies  >14 publications with SIRPA and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >12 publications with SIRPA and Cardiovascular Diseases
 Breast Neoplasms Antibodies  >9 publications with SIRPA and Breast Neoplasms
 Necrosis Antibodies  >7 publications with SIRPA and Necrosis
 Kidney Diseases Antibodies  >4 publications with SIRPA and Kidney Diseases
 Congenital Abnormalities Antibodies  >4 publications with SIRPA and Congenital Abnormalities
 Disease Models, Animal Antibodies  >4 publications with SIRPA and Disease Models, Animal
 Anemia Antibodies  >4 publications with SIRPA and Anemia
Organs/Tissues associated with SIRPA recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >68 publications with SIRPA and Blood
 Bone Antibodies  >19 publications with SIRPA and Bone
 Muscle Antibodies  >16 publications with SIRPA and Muscle
 Vascular Antibodies  >16 publications with SIRPA and Vascular
 Prostate Antibodies  >13 publications with SIRPA and Prostate
 Eye Antibodies  >9 publications with SIRPA and Eye
 Bone Marrow Antibodies  >9 publications with SIRPA and Bone Marrow
 Skin Antibodies  >8 publications with SIRPA and Skin
 Embryonic Tissue Antibodies  >6 publications with SIRPA and Embryonic Tissue
 Heart Antibodies  >6 publications with SIRPA and Heart
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