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SELENBP1 recombinant protein :: Selenium Binding Protein 1 (SELENBP1) Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS2011654 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
0.01 mg  /  $145 +1 FREE 8GB USB
0.05 mg  /  $280 +1 FREE 8GB USB
0.1 mg  /  $440 +1 FREE 8GB USB
0.2 mg  /  $540 +1 FREE 8GB USB
0.5 mg  /  $1,040 +1 FREE 8GB USB
1 mg  /  $1,545 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Selenium Binding Protein 1 (SELENBP1), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Selenium Binding Protein 1 (SELENBP1)

 Product Gene Name   

SELENBP1 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF-TVDVD PKSPQYCQVI HRLPMPNLKD ELHHSGWNTC SSCFGDSTKS RTKLVLPSLI SSRIYVVDVG SEPRAPKLHK VIEPKDIHAK CELAFLHTSH CLASGEVMIS SLGDVKGNGK GGFVLLDGET FEVKGTWERP GGAAPLGYDF WYQPRHNVMI STEWAAPNVL RDGFNPADVE AGLYGSHLYV WDWQRHEIVQ TLSLKDGLIP LEIRFLHNPD AAQGFVGCAL SSTIQRFYKN EGGTWS
 Chromosome Location    Chromosome: 1; NC_000001.10 (151336778..151345210, complement). Location: 1q21.3
 OMIM    604188
 3D Structure    ModBase 3D Structure for Q13228
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 Host    E Coli
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 Purity/Purification    > 95%
 Form/Format    Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH 8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
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 Organism    Homo sapiens (Human)
 Expression System    Prokaryotic expression
 Residues    Thr46~Ser286 (Accession # Q13228) with N-terminal His-Tag
 Endotoxin Level    <1.0EU per 1ug (determined by the LAL method)
 Reconstitution    Reconstitute in ddH2O
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 Preparation and Storage    Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
 ISO Certification    Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
 Supply Chain Verification    Manufactured in an a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Other Notes    Small volumes of SELENBP1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for SELENBP1 recombinant protein

   About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
 Applications Tested/Suitable for SELENBP1 recombinant protein   

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

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 SDS-PAGE of SELENBP1 recombinant protein    SELENBP1 recombinant protein SDS-PAGE image
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 SDS-PAGE of SELENBP1 recombinant protein    [ DESCRIPTION]
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10 kDa, 14 kDa, 18 kDa, 22 kDa, 26 kDa, 33 kDa, 44 kDa and 70 kDa.
Double intensity bands: The 26 kDa, 18 kDa, 10 kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
[ STORAGE BUFFER]
62.5mM Tris-H3P04 (pH 7.5 at 25C), 1 mM EDTA, 2% (w/v) SDS, 100mM DTT, 1 mM NaN3, 0.01 % (w/v) bromo-phenol blue and 33% (v/v) glycerol.
[APPLICATION]
Accurate protein sizing on SDS-polyacrylamide gels and Western blots.
[USAGE]
1. Allow the Marker to reach room temperature and thoroughly mix before use. This will ensure that any solids that may have precipitated at -20C have returned to solution. Do not boil!
2. Load the following volumes of the Marker on a SDS-polyacrylamide gel.
[ LOADING VOLUMES]
3~5uL per well for mini gel; 7uL per well for large gel.
Use the same volumes for Western blotting applications. The loading volumes listed above are recommended for gels with a thickness of 0.75~1.0mm. The loading volume should be double for 1.5mm thick gels.
[ QUALITY CONTROL]
The protein marker, with molecular weight shifts of < or = 5% and minimal band broadening, are confirmed by migration in SDS-PAGE system. Electrophoresis of the Marker on a 13~18% Tris-glycine SDS-polyacrylamide gel resolves 8 individual bands.
[IMPORTANT NOTES]
1. The protein marker is conveniently packaged and ready to use. There is no need to heat, dilute or add reducing agents. Do not boil, which may cause band degradation.
2. The molecular weights of the proteins have a lot-to-Iot variation of ~5%.
3. These protein markers are for SDS-PAGE and should not to be used for native electrophoresis or a native gel. These markers are denatured and have SDS in the storage buffer.
4. Don't load too much protein. See recommended load volumes in the manual.
5. Check % gel that is being used if the lands miss. Depending on gel type and/or percentage, you may not see all of the bands. For example, one would not see the smallest bands of the standard on a very low % gel. A high % gel may not resolve the higher MW bands. Homogeneous low percentage gels are recommended for analysis of large proteins and high percentage gels for analysis of small proteins. In high percentage gels (13~18%) large proteins (22~70kDa) can separate, while in low percentage gels (4~8%) small proteins (14 and 10kDa) will migrate with the tracking dye. Longer transfer times or higher transfer voltages may be required for Western blotting of large (>100kDa) proteins.
6. If additional bands are observed in the gel image of the protein ladder, this might be caused by DTT oxidation in the storage buffer.
7. If proteins show a poor transfer, increase voltage, current or length of time for transfer, and pay attention to the SDS from the transfer buffer. SDS will cause the proteins to bind less efficiently to membranes because it disrupts the hydrophobic interaction between the membrane and the protein. If SDS is present in transfer buffer, make sure that there is no more than 0.02% in buffer.
8. Alcohol enhances hydrophobic binding to membrane. Not enough alcohol may prevent binding.
[ SHELF LIFE]
Storage: -20 degree C, 1 year at -20 degree C. If the product has been stored as directed for one year, its shelf life maybe extended by adding dithiothreitol (Dn) to approximately 50mM.
SELENBP1 recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for SELENBP1. It may not necessarily be applicable to this product.
 NCBI GI #    385137128
 NCBI GeneID    8991
 NCBI Accession #    NP_001245217.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001258288.1 [Other Products]
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 UniProt Primary Accession #    Q13228 [Other Products]
 UniProt Secondary Accession #    Q49AQ8; Q96GX7; A6NML9; B2RDR3; B4DKP6; B4E1F3 [Other Products]
 UniProt Related Accession #    Q13228 [Other Products]
 Molecular Weight    28.3kDa
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 NCBI Official Full Name    selenium-binding protein 1 isoform 2
 NCBI Official Synonym Full Names    selenium binding protein 1
 NCBI Official Symbol    SELENBP1 [Similar Products]
 NCBI Official Synonym Symbols   
LPSB; SP56; hSBP; SBP56; HEL-S-134P
[Similar Products]
 NCBI Protein Information    selenium-binding protein 1; 56 kDa selenium-binding protein; epididymis secretory sperm binding protein Li 134P
 UniProt Protein Name    Selenium-binding protein 1
 UniProt Synonym Protein Names   
56 kDa selenium-binding protein
 Protein Family    Selenium-binding protein
 UniProt Gene Name    SELENBP1 [Similar Products]
 UniProt Synonym Gene Names    SBP; SBP56; SP56 [Similar Products]
 UniProt Entry Name    SBP1_HUMAN
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 NCBI Summary for SELENBP1    This gene encodes a member of the selenium-binding protein family. Selenium is an essential nutrient that exhibits potent anticarcinogenic properties, and deficiency of selenium may cause certain neurologic diseases. The effects of selenium in preventing cancer and neurologic diseases may be mediated by selenium-binding proteins, and decreased expression of this gene may be associated with several types of cancer. The encoded protein may play a selenium-dependent role in ubiquitination/deubiquitination-mediated protein degradation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2012]
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 UniProt Comments for SELENBP1    SELENBP1: Selenium-binding protein which may be involved in the sensing of reactive xenobiotics in the cytoplasm. May be involved in intra-Golgi protein transport. Belongs to the selenium-binding protein family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: extracellular space; membrane; nucleolus; cytosol

Molecular Function: selenium binding; protein binding

Biological Process: protein transport
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 Research Articles on SELENBP1    1. The expression level of SELENBP1 could be an important marker for predicting survival and effectiveness of selenium supplementation in breast cancer.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with SELENBP1 recombinant proteinPathways associated with SELENBP1 recombinant protein
 Reference Product  PubMed Publications
 ENO1 recombinant protein  >2 publications with SELENBP1 and ENO1
 USP33 recombinant protein  >1 publications with SELENBP1 and USP33
 Products by Pathway  Pathway Diagram
 Selenium Metabolism And Selenoproteins Pathway antibodies  Selenium Metabolism And Selenoproteins Pathway Diagram
Diseases associated with SELENBP1 recombinant proteinOrgans/Tissues associated with SELENBP1 recombinant protein
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >24 publications with SELENBP1 and Neoplasms
 Adenocarcinoma Antibodies  >9 publications with SELENBP1 and Adenocarcinoma
 Schizophrenia Antibodies  >5 publications with SELENBP1 and Schizophrenia
 Inflammation Antibodies  >4 publications with SELENBP1 and Inflammation
 Colorectal Neoplasms Antibodies  >4 publications with SELENBP1 and Colorectal Neoplasms
 Liver Diseases Antibodies  >4 publications with SELENBP1 and Liver Diseases
 Kidney Diseases Antibodies  >3 publications with SELENBP1 and Kidney Diseases
 Stomach Neoplasms Antibodies  >3 publications with SELENBP1 and Stomach Neoplasms
 Carcinoma, Hepatocellular Antibodies  >3 publications with SELENBP1 and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >3 publications with SELENBP1 and Liver Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >9 publications with SELENBP1 and Liver
 Blood Antibodies  >9 publications with SELENBP1 and Blood
 Lung Antibodies  >7 publications with SELENBP1 and Lung
 Kidney Antibodies  >6 publications with SELENBP1 and Kidney
 Brain Antibodies  >6 publications with SELENBP1 and Brain
 Prostate Antibodies  >4 publications with SELENBP1 and Prostate
 Intestine Antibodies  >4 publications with SELENBP1 and Intestine
 Eye Antibodies  >3 publications with SELENBP1 and Eye
 Heart Antibodies  >3 publications with SELENBP1 and Heart
 Stomach Antibodies  >3 publications with SELENBP1 and Stomach
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