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PIM1 recombinant protein :: Serine/threonine-protein kinase pim-1 (PIM1) Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS957553 PIM1 recombinant protein
Unit / Price
0.5 mg (E-Coli)  /  $1,175 +1 FREE 8GB USB
0.05 mg (Baculovirus)  /  $1,175 +1 FREE 8GB USB
0.5 mg (Yeast)  /  $1,395 +1 FREE 8GB USB
0.05 mg (Mammalian-Cell)  /  $1,395 +1 FREE 8GB USB
1 mg (E-Coli)  /  $1,890 +2 FREE 8GB USB
0.1 mg (Baculovirus)  /  $1,890 +2 FREE 8GB USB
1 mg (Yeast)  /  $2,355 +2 FREE 8GB USB
0.1 mg (Mammalian-Cell)  /  $2,355 +2 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Serine/threonine-protein kinase pim-1 (PIM1), Recombinant Protein

 Also Known As   

Recombinant Human Serine/threonine-protein kinase pim-1 (PIM1)

 Product Synonym Names    Serine/threonine-protein kinase pim-1; EC=2.7.11.1
 Product Gene Name   

PIM1 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence Positions    1-404
 Sequence    MPHEPHEPLT PPFSALPDPA GAPSRRQSRQ RPQLSSDSPS AFRASRSHSR NATRSHSHSH SPRHSLRHSP GSGSCGSSSG HRPCADILEV GMLLSKINSL AHLRAAPCND LHATKLAPGK EKEPLESQYQ VGPLLGSGGF GSVYSGIRVS DNLPVAIKHV EKDRISDWGE LPNGTRVPME VVLLKKVSSG FSGVIRLLDW FERPDSFVLI LERPEPVQDL FDFITERGAL QEELARSFFW QVLEAVRHCH NCGVLHRDIK DENILIDLNR GELKLIDFGS GALLKDTVYT DFDGTRVYSP PEWIRYHRYH GRSAAVWSLG ILLYDMVCGD IPFEHDEEII RGQVFFRQRV SSECQHLIRW CLALRPSDRP TFEEIQNHPW MQDVLLPQET AEIHLHSLSP GPSK
 OMIM    164960
 3D Structure    ModBase 3D Structure for P11309
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 Host    E Coli or Yeast or Baculovirus or Mammalian Cell
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 Purity/Purification    >90%
 Form/Format    Liquid containing glycerol; lyophilization may be available upon request.
 Tag Information    This protein contains an N-terminal tag and may also contain a C-terminal Myc-tag. N-terminal host tags may vary (His, His-SUMO, His-B2M, GST). Tag types are determined by various factors including tag-protein stability and, therefore, are subject to change; please inquire for tag information. Additional charge for Tag removal. Tag removal service standardly refers to removal of N-terminal Tag. Any C-terminal Myc-tag may remain intact unless removal of the C-terminal Myc-tag is specifically requested. In most cases tags can be successfully removed. If the lab concludes the protein is no longer stable after tag removal then your protein will be supplied with the tag intact, and you will not be charged for tag removal service.
 Sterility    Sterile filter available upon request.
 Endotoxin    Low endotoxin available upon request.
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 Species    Homo sapiens (Human)
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 Preparation and Storage    Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of PIM1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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NCBI/Uniprot data below describe general gene information for PIM1. It may not necessarily be applicable to this product.
 NCBI GI #    342307046
 NCBI GeneID    5292
 NCBI Accession #    NP_001230115.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001243186.1 [Other Products]
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 UniProt Primary Accession #    P11309 [Other Products]
 UniProt Secondary Accession #    Q38RT9; Q5T7H7; Q96RG3 [Other Products]
 UniProt Related Accession #    P11309 [Other Products]
 Molecular Weight    35,686 Da
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 NCBI Official Full Name    serine/threonine-protein kinase pim-1 isoform 1
 NCBI Official Synonym Full Names    Pim-1 proto-oncogene, serine/threonine kinase
 NCBI Official Symbol    PIM1 [Similar Products]
 NCBI Official Synonym Symbols   
PIM
[Similar Products]
 NCBI Protein Information    serine/threonine-protein kinase pim-1; Oncogene PIM1; pim-1 kinase 44 kDa isoform; pim-1 oncogene (proviral integration site 1); proto-oncogene serine/threonine-protein kinase pim-1
 UniProt Protein Name    Serine/threonine-protein kinase pim-1
 Protein Family    Serine/threonine-protein kinase
 UniProt Gene Name    PIM1 [Similar Products]
 UniProt Entry Name    PIM1_HUMAN
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 NCBI Summary for PIM1    The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons (PMIDs:16186805, 1825810).[provided by RefSeq, Aug 2011]
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 UniProt Comments for PIM1    Pim1: a proto-oncogene serine/threonine kinase involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerced by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl- X(L)/BCL2L1. Phosphorylation of ASK1 an other proapoptotic protein, by PIM1, significantly decreases ASK1 kinase activity and inhibits MAP3K5-mediated phosphorylation of JNK and JNK/p38MAPK subsequently reducing caspase-3 activation and cell apoptosis. Stimulates cell cycle progression at the G1-S and G2-M transitions by phosphorylation of CDC25A and CDC25C. Phosphorylation of p21Cip1, a regulator of cell cycle progression at G1, results in the relocation of p21Cip1 to the cytoplasm and enhanced p21Cip1 protein stability. Promote cell cycle progression and tumorigenesis by down-regulating expression of a regulator of cell cycle progression, p27Kip1, at both transcriptional and post- translational levels. Phosphorylation of p27Kip1,induces 14-3-3- proteins binding, nuclear export and proteasome-dependent degradation. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Acts also as a regulator of homing and migration of bone marrow cells involving functional interaction with the CXCL12-CXCR4 signaling axis. Isoform 2 is isolated as a monomer whereas isoform 1 complexes with other proteins. Binds to RP9. Isoform 1, but not isoform 2, binds BMX. Isoform 2 interacts with p27Kip1 and FOXO3. Interacts with BAD. Interacts with PPP2CA; this interaction promotes dephosphorylation of PIM1, ubiquitination and proteasomal degradation. Interacts with HSP90, this interaction stabilizes PIM1 protein levels. Ubiquitinated form interacts with HSP70 and promotes its proteosomal degradation. Strongly induced in leukocytes by the JAK/STAT pathway in response to cytokines. Induced by different cellular stresses, heat shock and cytotoxic agents. Expressed primarily in cells of the hematopoietic and germline lineages. 2 isoforms of the human protein are produced by alternative initiation. Both isoforms are expressed in prostate cancer cell lines.

Protein type: Protein kinase, CAMK; Kinase, protein; Oncoprotein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; CAMK group; PIM family

Chromosomal Location of Human Ortholog: 6p21.2

Cellular Component: cytoplasm; plasma membrane; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; manganese ion binding; transcription factor binding; ATP binding; ribosomal small subunit binding

Biological Process: cell proliferation; apoptosis; negative regulation of transcription factor activity; multicellular organismal development; protein amino acid autophosphorylation; regulation of mitotic cell cycle; cell cycle; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; protein amino acid phosphorylation; hyaluronan metabolic process; negative regulation of apoptosis
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 Research Articles on PIM1    1. Our study indicates that the expression of Pim kinases is physiologically related to DNA-PKcs and ATM in ECs.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PIM1 recombinant proteinPathways associated with PIM1 recombinant protein
 Reference Product  PubMed Publications
 BAD recombinant protein  >14 publications with PIM1 and BAD
 CDKN1A recombinant protein  >7 publications with PIM1 and CDKN1A
 CDC25A recombinant protein  >4 publications with PIM1 and CDC25A
 NFATC1 recombinant protein  >3 publications with PIM1 and NFATC1
 SND1 recombinant protein  >2 publications with PIM1 and SND1
 SNX6 recombinant protein  >1 publications with PIM1 and SNX6
 NUMA1 recombinant protein  >1 publications with PIM1 and NUMA1
 Products by Pathway  Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
 IL-5 Signaling Pathway antibodies  IL-5 Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Role Of Calcineurin-dependent NFAT Signaling In Lymphocytes Pathway antibodies  Role Of Calcineurin-dependent NFAT Signaling In Lymphocytes Pathway Diagram
 Validated Targets Of C-MYC Transcriptional Activation Pathway antibodies  Validated Targets Of C-MYC Transcriptional Activation Pathway Diagram
Diseases associated with PIM1 recombinant proteinOrgans/Tissues associated with PIM1 recombinant protein
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >185 publications with PIM1 and Neoplasms
 Cell Transformation, Neoplastic Antibodies  >33 publications with PIM1 and Cell Transformation, Neoplastic
 Carcinoma Antibodies  >27 publications with PIM1 and Carcinoma
 Prostatic Neoplasms Antibodies  >26 publications with PIM1 and Prostatic Neoplasms
 Disease Models, Animal Antibodies  >22 publications with PIM1 and Disease Models, Animal
 Neoplasms, Experimental Antibodies  >19 publications with PIM1 and Neoplasms, Experimental
 Cardiovascular Diseases Antibodies  >17 publications with PIM1 and Cardiovascular Diseases
 Inflammation Antibodies  >15 publications with PIM1 and Inflammation
 Liver Diseases Antibodies  >11 publications with PIM1 and Liver Diseases
 Adenocarcinoma Antibodies  >10 publications with PIM1 and Adenocarcinoma
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >100 publications with PIM1 and Blood
 Prostate Antibodies  >38 publications with PIM1 and Prostate
 Bone Antibodies  >26 publications with PIM1 and Bone
 Muscle Antibodies  >25 publications with PIM1 and Muscle
 Heart Antibodies  >21 publications with PIM1 and Heart
 Bone Marrow Antibodies  >20 publications with PIM1 and Bone Marrow
 Liver Antibodies  >19 publications with PIM1 and Liver
 Vascular Antibodies  >14 publications with PIM1 and Vascular
 Lung Antibodies  >12 publications with PIM1 and Lung
 Thymus Antibodies  >11 publications with PIM1 and Thymus
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