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SLC30A8 recombinant protein :: Solute Carrier Family 30 Member 8 (SLC30A8) Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS2009993 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
0.01 mg  /  $110 +1 FREE 8GB USB
0.05 mg  /  $195 +1 FREE 8GB USB
0.1 mg  /  $270 +1 FREE 8GB USB
0.2 mg  /  $340 +1 FREE 8GB USB
0.5 mg  /  $645 +1 FREE 8GB USB
1 mg  /  $955 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Solute Carrier Family 30 Member 8 (SLC30A8), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Solute Carrier Family 30 Member 8 (SLC30A8)

 Product Gene Name   

SLC30A8 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    The sequence of the target protein is listed below.
MEFLERTYLV NDKAAKMYAF TLESVELQQK PVNKDQCPRE RPEELESGGM YHCHSGSKPT EKGANEYA YA KWKLCSASAI
 Chromosome Location    Chromosome: 8; NC_000008.10 (117962512..118188953). Location: 8q24.11
 OMIM    125853
 3D Structure    ModBase 3D Structure for Q8IWU4
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 Host    E Coli
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 Purity/Purification    > 95%
 Form/Format    Supplied as lyophilized form in PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.05% sarcosyl and preservative.
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 Organism    Homo sapiens (Human)
 Expression System    Prokaryotic expression
 Residues    Met1~Ile80 with N-terminal His-Tag
 Accession #    Q8IWU4
 Endotoxin Level    <1.0EU per 1ug (determined by the LAL method)
 Reconstitution    Reconstitute in sterile PBS, pH7.2-pH7.4.
 Predicted isoelectric point    5.9
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 Preparation and Storage    Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
 Other Notes    Small volumes of SLC30A8 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for SLC30A8 recombinant protein

   About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
 Applications Tested/Suitable for SLC30A8 recombinant protein   

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

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 SDS-PAGE of SLC30A8 recombinant protein    SLC30A8 recombinant protein SDS-PAGE image
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 SDS-PAGE of SLC30A8 recombinant protein    [ DESCRIPTION]
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10 kDa, 14 kDa, 18 kDa, 22 kDa, 26 kDa, 33 kDa, 44 kDa and 70 kDa.
Double intensity bands: The 26 kDa, 18 kDa, 10 kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
[ STORAGE BUFFER]
62.5mM Tris-H3P04 (pH 7.5 at 25C), 1 mM EDTA, 2% (w/v) SDS, 100mM DTT, 1 mM NaN3, 0.01 % (w/v) bromo-phenol blue and 33% (v/v) glycerol.
[APPLICATION]
Accurate protein sizing on SDS-polyacrylamide gels and Western blots.
[USAGE]
1. Allow the Marker to reach room temperature and thoroughly mix before use. This will ensure that any solids that may have precipitated at -20C have returned to solution. Do not boil!
2. Load the following volumes of the Marker on a SDS-polyacrylamide gel.
[ LOADING VOLUMES]
3~5uL per well for mini gel; 7uL per well for large gel.
Use the same volumes for Western blotting applications. The loading volumes listed above are recommended for gels with a thickness of 0.75~1.0mm. The loading volume should be double for 1.5mm thick gels.
[ QUALITY CONTROL]
The protein marker, with molecular weight shifts of < or = 5% and minimal band broadening, are confirmed by migration in SDS-PAGE system. Electrophoresis of the Marker on a 13~18% Tris-glycine SDS-polyacrylamide gel resolves 8 individual bands.
[IMPORTANT NOTES]
1. The protein marker is conveniently packaged and ready to use. There is no need to heat, dilute or add reducing agents. Do not boil, which may cause band degradation.
2. The molecular weights of the proteins have a lot-to-Iot variation of ~5%.
3. These protein markers are for SDS-PAGE and should not to be used for native electrophoresis or a native gel. These markers are denatured and have SDS in the storage buffer.
4. Don't load too much protein. See recommended load volumes in the manual.
5. Check % gel that is being used if the lands miss. Depending on gel type and/or percentage, you may not see all of the bands. For example, one would not see the smallest bands of the standard on a very low % gel. A high % gel may not resolve the higher MW bands. Homogeneous low percentage gels are recommended for analysis of large proteins and high percentage gels for analysis of small proteins. In high percentage gels (13~18%) large proteins (22~70kDa) can separate, while in low percentage gels (4~8%) small proteins (14 and 10kDa) will migrate with the tracking dye. Longer transfer times or higher transfer voltages may be required for Western blotting of large (>100kDa) proteins.
6. If additional bands are observed in the gel image of the protein ladder, this might be caused by DTT oxidation in the storage buffer.
7. If proteins show a poor transfer, increase voltage, current or length of time for transfer, and pay attention to the SDS from the transfer buffer. SDS will cause the proteins to bind less efficiently to membranes because it disrupts the hydrophobic interaction between the membrane and the protein. If SDS is present in transfer buffer, make sure that there is no more than 0.02% in buffer.
8. Alcohol enhances hydrophobic binding to membrane. Not enough alcohol may prevent binding.
[ SHELF LIFE]
Storage: -20 degree C, 1 year at -20 degree C. If the product has been stored as directed for one year, its shelf life maybe extended by adding dithiothreitol (Dn) to approximately 50mM.
SLC30A8 recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for SLC30A8. It may not necessarily be applicable to this product.
 NCBI GI #    289803003
 NCBI GeneID    169026
 NCBI Accession #    NP_001166282.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001172811.1 [Other Products]
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 UniProt Primary Accession #    Q8IWU4 [Other Products]
 UniProt Secondary Accession #    Q8TCL3; A0AVP9; A5YM39; B4DPE0 [Other Products]
 UniProt Related Accession #    Q8IWU4 [Other Products]
 Molecular Weight    10.7kDa
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 NCBI Official Full Name    zinc transporter 8 isoform b
 NCBI Official Synonym Full Names    solute carrier family 30 (zinc transporter), member 8
 NCBI Official Symbol    SLC30A8 [Similar Products]
 NCBI Official Synonym Symbols   
ZNT8; ZnT-8
[Similar Products]
 NCBI Protein Information    zinc transporter 8; zinc transporter ZnT-8
 UniProt Protein Name    Zinc transporter 8
 UniProt Synonym Protein Names   
Solute carrier family 30 member 8
 Protein Family    Zinc transporter
 UniProt Gene Name    SLC30A8 [Similar Products]
 UniProt Synonym Gene Names    ZNT8; ZnT-8 [Similar Products]
 UniProt Entry Name    ZNT8_HUMAN
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 NCBI Summary for SLC30A8    The protein encoded by this gene is a zinc efflux transporter involved in the accumulation of zinc in intracellular vesicles. This gene is expressed at a high level only in the pancreas, particularly in islets of Langerhans. The encoded protein colocalizes with insulin in the secretory pathway granules of the insulin-secreting INS-1 cells. Allelic variants of this gene exist that confer susceptibility to diabetes mellitus, noninsulin-dependent (NIDDM). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010]
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 UniProt Comments for SLC30A8    SLC30A8: Facilitates the accumulation of zinc from the cytoplasm into intracellular vesicles, being a zinc-efflux transporter. May be a major component for providing zinc to insulin maturation and/or storage processes in insulin-secreting pancreatic beta- cells. Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, SLC family; Vesicle; Membrane protein, integral; Transporter; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 8q24.11

Cellular Component: Golgi apparatus; secretory granule membrane; cytoplasmic membrane-bound vesicle; plasma membrane; integral to membrane; secretory granule

Molecular Function: protein homodimerization activity; zinc ion binding; zinc ion transmembrane transporter activity

Biological Process: sequestering of zinc ion; cellular protein metabolic process; insulin secretion; cellular zinc ion homeostasis; response to glucose stimulus; positive regulation of insulin secretion; zinc ion transport; glucose homeostasis; transmembrane transport

Disease: Diabetes Mellitus, Noninsulin-dependent
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 Research Articles on SLC30A8    1. ZnT8A were more common and more persistent in patients with LADA compared to adult-onset type 1 diabetes, but their presence was not associated with specific phenotypic characteristics.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with SLC30A8 recombinant proteinPathways associated with SLC30A8 recombinant protein
 Reference Product  PubMed Publications
 HHEX recombinant protein  >80 publications with SLC30A8 and HHEX
 CDKAL1 recombinant protein  >76 publications with SLC30A8 and CDKAL1
 TCF7L2 recombinant protein  >74 publications with SLC30A8 and TCF7L2
 IGF2BP2 recombinant protein  >69 publications with SLC30A8 and IGF2BP2
 KCNJ11 recombinant protein  >46 publications with SLC30A8 and KCNJ11
 FTO recombinant protein  >40 publications with SLC30A8 and FTO
 WFS1 recombinant protein  >25 publications with SLC30A8 and WFS1
 MTNR1B recombinant protein  >13 publications with SLC30A8 and MTNR1B
 Products by Pathway  Pathway Diagram
 Metal Ion SLC Transporters Pathway antibodies  Metal Ion SLC Transporters Pathway Diagram
 SLC-mediated Transmembrane Transport Pathway antibodies  SLC-mediated Transmembrane Transport Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram
 Transport Of Glucose And Other Sugars, Bile Salts And Organic Acids, Metal Ions And Amine Compounds Pathway antibodies  Transport Of Glucose And Other Sugars, Bile Salts And Organic Acids, Metal Ions And Amine Compounds Pathway Diagram
 Zinc Efflux And Compartmentalization By The SLC30 Family Pathway antibodies  Zinc Efflux And Compartmentalization By The SLC30 Family Pathway Diagram
 Zinc Transporters Pathway antibodies  Zinc Transporters Pathway Diagram
Diseases associated with SLC30A8 recombinant proteinOrgans/Tissues associated with SLC30A8 recombinant protein
 Disease Name  Pubmed Publications
 Diabetes Mellitus, Type 2 Antibodies  >123 publications with SLC30A8 and Diabetes Mellitus, Type 2
 Immune System Diseases Antibodies  >59 publications with SLC30A8 and Immune System Diseases
 Diabetes Mellitus, Experimental Antibodies  >5 publications with SLC30A8 and Diabetes Mellitus, Experimental
 Arteriosclerosis Antibodies  >4 publications with SLC30A8 and Arteriosclerosis
 Kidney Diseases Antibodies  >4 publications with SLC30A8 and Kidney Diseases
 Inflammation Antibodies  >3 publications with SLC30A8 and Inflammation
 Hypertension Antibodies  >2 publications with SLC30A8 and Hypertension
 Hypertrophy Antibodies  >2 publications with SLC30A8 and Hypertrophy
 Acidosis Antibodies  >1 publications with SLC30A8 and Acidosis
 Congenital Abnormalities Antibodies  >1 publications with SLC30A8 and Congenital Abnormalities
 Organ/Tissue Name  Pubmed Publications
 Pancreas Antibodies  >51 publications with SLC30A8 and Pancreas
 Kidney Antibodies  >8 publications with SLC30A8 and Kidney
 Liver Antibodies  >5 publications with SLC30A8 and Liver
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