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SCF recombinant protein :: Stem Cell Factor Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS553178 SCF recombinant protein
Unit / Price
0.002 mg  /  $240 +1 FREE 8GB USB
0.01 mg  /  $430 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Stem Cell Factor (SCF), Recombinant Protein

★Popular Item★
 Also Known As   

Mouse Stem Cell Factor Recombinant

 Product Synonym Names    Mouse Stem cell Factor; kit ligand; kitlg; mgf; stem cell factor; scf
 Product Gene Name   

SCF recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MKKTQTWIIT CIYLQLLLFN PLVKTKEICG NPVTDNVKDI TKLVANLPND YMITLNYVAG MDVLPSHCWL RDMVIQLSLS LTTLLDKFSN ISEGLSNYSI IDKLGKIVDD LVLCMEENAP KNIKESPKRP ETRSFTPEEF FSIFNRSIDA FKDFMVASDT SDCVLSSTLG PEKDSRVSVT KPFMLPPVAA SSLRNDSSSS NRKAAKAPED SGLQWTAMAL PALISLVIGF AFGALYWKKK QSSLTRAVEN IQINEEDNEI SMLQQKEREF QEV
 3D Structure    ModBase 3D Structure for P20826
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 Host    Insect Cells
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 Purity/Purification    >95%, as determined by SDS-PAGE and HPLC
 Form/Format    Recombinant Stem cell factor was lyophilized from a 0.2 um filtered solution in 20mM Tris, 5% trehalose, pH 7.5.
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 Domain    Four helix core
 Host Note    Optimized DNA sequence encoding Mouse SCF mature chain was expressed in Insect cells.
 Endotoxin    Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
 Reconstitution    A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
 Molecular Weight Note    Native Mouse Stem Cell Factor is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 18 kDa.

Recombinant SCF is a disulfide-linked monomer protein consisting of 165 amino acid residue subunits, and due to glycosylation migrates as an approximately 23 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
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 Preparation and Storage    The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.
 Other Notes    Small volumes of SCF recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for SCF recombinant protein

   SCF is a stromal cell- derived cytokine synthesized by fibroblasts and other cell types. SCF promotes proliferation and early differentiation of cells at the level of multipotential stem cells. It has been suggested that SCF is essential for optimal production of various hematopoietic lineages, mainly because of its ability to prevent apoptosis when it co- stimulates with other cytokines. The receptor for SCF, designated SCFR (CD117), is the oncogene designated as KIT. The biological activities of SCF are synergised considerably by colony stimulating factors GM-CSF and G-CSF, and also by IL7, Epo and some other growth and differentiation factors. In combination with IL7 SCF stimulates the proliferation of pre-B-cells. SCF is also a potent chemoattractant for cells (see also: Chemotaxis), for example, mast cells, expressing the kit receptor. One response to SCF in these cells is a characteristic rearrangement of the actin filaments of the cytoskeleton.
 Product Categories/Family for SCF recombinant protein    Cytokines
NCBI/Uniprot data below describe general gene information for SCF. It may not necessarily be applicable to this product.
 NCBI GI #    7305267
 NCBI GeneID    17311
 NCBI Accession #    NP_038626.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_013598.2 [Other Products]
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 UniProt Primary Accession #    P20826 [Other Products]
 UniProt Secondary Accession #    P97332; Q3TNJ7; Q62524; Q64222; Q921N5 [Other Products]
 UniProt Related Accession #    P20826; Q61854; Q64384; Q78ED8; Q8C9K1 [Other Products]
 Molecular Weight    30,645 Da
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 NCBI Official Full Name    kit ligand
 UniProt Protein Name    Kit ligand
 UniProt Synonym Protein Names   
Hematopoietic growth factor KL; Mast cell growth factor; MGF; Steel factor; Stem cell factor; SCF; c-Kit ligand
 UniProt Gene Name    Kitlg [Similar Products]
 UniProt Synonym Gene Names    Kitl; Mgf; Sl; Slf; MGF; SCF; sKITLG [Similar Products]
 UniProt Entry Name    SCF_MOUSE
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 UniProt Comments for SCF    SCF: Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. Homodimer, non-covalently linked (Probable). Heterotetramer with KIT, binding two KIT molecules; thereby mediates KIT dimerization and subsequent activation by autophosphorylation. Belongs to the SCF family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: extracellular space; cytoskeleton; membrane; cytoplasm; integral to membrane; extracellular region; plasma membrane

Molecular Function: protein binding; growth factor activity; cytokine activity; stem cell factor receptor binding

Biological Process: embryonic hemopoiesis; positive regulation of leukocyte migration; germ cell programmed cell death; germ cell development; positive regulation of MAP kinase activity; positive regulation of peptidyl-tyrosine phosphorylation; ovarian follicle development; positive regulation of melanocyte differentiation; negative regulation of mast cell apoptosis; positive regulation of cell proliferation; positive regulation of Ras protein signal transduction; positive regulation of myeloid leukocyte differentiation; cell adhesion; neural crest cell migration; positive regulation of DNA replication; negative regulation of apoptosis
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Product References and Citations for SCF recombinant protein

   cardiomyocyte-specific overexpression of human Stem Cell Factor improves cardiac function and survival after myocardial infarction in mice .Circulation, Sep 2009; 120: 1065 - 1074.
Stem Cell Factor and interleukin-2/15 combine to enhance mapk-mediated proliferation of human natural killer cells .Blood, Mar 2009; 113: 2706 - 2714.
human Stem Cell Factor (scf) is a heparin-binding cytokine .J. Biochem., Mar 2009; 145: 275 - 278.
role of Stem Cell Factor and bone marrow-derived fibroblasts in airway remodeling .Am. J. Pathol., Feb 2009; 174: 390 - 400.
Stem Cell Factor signaling collaborates in the development of fip1l1/pdgfr induced chronic eosinophilic leukemia in a murine model .Blood (ASH Annual Meeting Abstracts), Nov 2008; 112: 470.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with SCF recombinant proteinDiseases associated with SCF recombinant protein
 Products by Pathway  Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Kit Receptor Signaling Pathway antibodies  Kit Receptor Signaling Pathway Diagram
 Melanogenesis Pathway antibodies  Melanogenesis Pathway Diagram
 Melanogenesis Pathway antibodies  Melanogenesis Pathway Diagram
 Pathways In Cancer antibodies  Pathways In Cancer Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >8 publications with SCF and Neoplasms
 Female Urogenital Diseases Antibodies  >5 publications with SCF and Female Urogenital Diseases
 Skin Diseases Antibodies  >4 publications with SCF and Skin Diseases
 Infertility Antibodies  >4 publications with SCF and Infertility
 Gonadal Disorders Antibodies  >4 publications with SCF and Gonadal Disorders
 Inflammation Antibodies  >4 publications with SCF and Inflammation
 Drug Toxicity Antibodies  >3 publications with SCF and Drug Toxicity
 Melanoma Antibodies  >3 publications with SCF and Melanoma
 Leukemia Antibodies  >3 publications with SCF and Leukemia
 Infertility, Male Antibodies  >2 publications with SCF and Infertility, Male
Organs/Tissues associated with SCF recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >23 publications with SCF and Bone
 Ovary Antibodies  >18 publications with SCF and Ovary
 Skin Antibodies  >17 publications with SCF and Skin
 Bone Marrow Antibodies  >12 publications with SCF and Bone Marrow
 Testis Antibodies  >10 publications with SCF and Testis
 Embryonic Tissue Antibodies  >7 publications with SCF and Embryonic Tissue
 Liver Antibodies  >6 publications with SCF and Liver
 Heart Antibodies  >4 publications with SCF and Heart
 Spleen Antibodies  >3 publications with SCF and Spleen
 Eye Antibodies  >2 publications with SCF and Eye
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