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CXCL12 recombinant protein :: Stromal Cell Derived Factor 1 beta Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS651060 CXCL12 recombinant protein
Unit / Price
0.01 mg  /  $655 +1 FREE 8GB USB
 
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 Product Name   

Stromal Cell Derived Factor 1 beta (CXCL12), Recombinant Protein

 Also Known As   

Stromal Cell Derived Factor 1 beta, Recombinant, Mouse (SDF1b, CXCL12b, Pre-B Cell Growth-stimulating Factor, PBSF, hIRH, Chemokine (C-X-C Motif) Ligand 12b, TPAR1, SCYB12, TLSF-b)

 Product Gene Name   

CXCL12 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 10; NC_000010.10 (44865605..44880545, complement). Location: 10q11.1
 OMIM    600835
 3D Structure    ModBase 3D Structure for P48061
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 Purity/Purification    Highly Purified
~99% (SDS-PAGE, HPLC)
 Form/Format    Supplied as a lyophilized powder. BSA free. Reconstitute with sterile dH2O to 0.1-1mg/ml.
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 Biological Activity    Determined measuring dose-dependent chemotaxis with human peripheral blood monocytes. A concentration range of 50-100ng/ml produced significant chemotaxis. SDF-1 is a CXC chemokine that binds to the chemokine receptor CXCR4. SDF-1 is widely expressed by cells of most tissues, including bone marrow stromal cells. SDF-1 is a chemoattractant for mononuclear cells and CD34+ hematopoietic progenitor cells.
 Endotoxin Level    0.1ng/ug
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 Preparation and Storage    Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
 Other Notes    Small volumes of CXCL12 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for CXCL12 recombinant protein

   SDF-1 (stromal cell-derived factor-1) is small cytokine belonging to the chemokine family that is officially designated Chemokine (C-X-C motif) ligand 12 (CXCL12). Stromal cell-derived factors 1-alpha and 1-beta are small cytokines that belong to the intercrine family, members of which activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF, or IL1. The intercrines are characterized by the presence of 4 conserved cysteines which form 2 disulfide bonds. They can be classified into 2 subfamilies. In the CC subfamily, which includes beta chemokine, the cysteine residues are adjacent to each other. In the CXC subfamily, which includes alpha chemokine, they are separated by an intervening amino acid. The SDF1 proteins belong to the latter group. SDF-1 is produced in two forms, SDF-1a/CXCL12a and SDF-1b/CXCL12b, by alternate splicing of the same gene.[2] Chemokines are characterized by the presence of four conserved cysteines, which form two disulfide bonds. The CXCL12 proteins belong to the group of CXC chemokines, whose initial pair of cysteines are separated by one intervening amino acid.

CXCL12 is strongly chemotactic for lymphocytes.[3][4][5][6] During embryogenesis it directs the migration of hematopoietic cells from foetal liver to bone marrow and the formation of large blood vessels. Mice which were knocked-out for CXCL12 gene were lethal before the birth or within just 1 hour of life. In adulthood CXCL12 plays an important role in angiogenesis by recruiting endothelial progenitor cells (EPC) from the bone morrow through a CXCR4 dependent mechanism.[7] It is this function of CXCL12 that makes it a very important factor in carcinogenesis and the neovascularisation linked to tumor progression. [8] CXCL12 was shown to be expressed in many tissues in mice (including brain, thymus, heart, lung, liver, kidney, spleen and bone marrow).
 Product Categories/Family for CXCL12 recombinant protein    Growth Factors, Cytokines; Growth Factors-Cell Factors
NCBI/Uniprot data below describe general gene information for CXCL12. It may not necessarily be applicable to this product.
 NCBI GI #    10834988
 NCBI GeneID    6387
 NCBI Accession #    NP_000600.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000609.5 [Other Products]
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 UniProt Primary Accession #    P48061 [Other Products]
 UniProt Secondary Accession #    Q5IT36; Q9H554; B2R4G0; E7EVL0 [Other Products]
 UniProt Related Accession #    P48061; Q2L985; Q2L986; Q2L988; Q6ICW0; Q8NF00 [Other Products]
 Molecular Weight    8.5kD, 72 amino acid residues [Similar Products]
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 NCBI Official Full Name    stromal cell-derived factor 1 isoform beta
 NCBI Official Synonym Full Names    chemokine (C-X-C motif) ligand 12
 NCBI Official Symbol    CXCL12 [Similar Products]
 NCBI Official Synonym Symbols   
IRH; PBSF; SDF1; TLSF; SDF1A; SDF1B; TPAR1; SCYB12
[Similar Products]
 NCBI Protein Information    stromal cell-derived factor 1; OTTHUMP00000019491; OTTHUMP00000019492; OTTHUMP00000216979; OTTHUMP00000216980; intercrine reduced in hepatomas; pre-B cell growth-stimulating factor
 UniProt Protein Name    Stromal cell-derived factor 1
 UniProt Synonym Protein Names   
C-X-C motif chemokine 12; Intercrine reduced in hepatomas; IRH; hIRH; Pre-B cell growth-stimulating factor
 Protein Family    Stromal cell-derived factor
 UniProt Gene Name    CXCL12 [Similar Products]
 UniProt Synonym Gene Names    SDF1; SDF1A; SDF1B [Similar Products]
 UniProt Entry Name    SDF1_HUMAN
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 NCBI Summary for CXCL12    This gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.
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 UniProt Comments for CXCL12    Function: Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the Lyn kinase. Stimulates migration of monocytes through its receptor, CXCR4, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through Lyn kinase. Ref.11 Ref.12 Ref.14

Subcellular location: Secreted.

Tissue specificity: Isoforms Alpha and Beta have a highest expression in liver, pancreas and spleen, while isoform Gamma has only been detected in heart. Ref.5

Post-translational modification: Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processsed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans. Ref.13

Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.

Sequence caution: The sequence CAC10202.1 differs from that shown. Reason: Erroneous gene model prediction.
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 Research Articles on CXCL12    1. prostate adenocarcinoma tumorigenesis requires a mix of -beta responsive and nonresponsive stromal cells and SDF-1
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CXCL12 recombinant proteinPathways associated with CXCL12 recombinant protein
 Reference Product  PubMed Publications
 CXCR4 recombinant protein  >2216 publications with CXCL12 and CXCR4
 CD4 recombinant protein  >274 publications with CXCL12 and CD4
 MMP2 recombinant protein  >12 publications with CXCL12 and MMP2
 DPP4 recombinant protein  >9 publications with CXCL12 and DPP4
 CTSG recombinant protein  >3 publications with CXCL12 and CTSG
 FN1 recombinant protein  >1 publications with CXCL12 and FN1
 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 CXCR4-mediated Signaling Events Pathway antibodies  CXCR4-mediated Signaling Events Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
Diseases associated with CXCL12 recombinant proteinOrgans/Tissues associated with CXCL12 recombinant protein
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >949 publications with CXCL12 and Neoplasms
 Inflammation Antibodies  >346 publications with CXCL12 and Inflammation
 Cardiovascular Diseases Antibodies  >318 publications with CXCL12 and Cardiovascular Diseases
 Carcinoma Antibodies  >265 publications with CXCL12 and Carcinoma
 Neoplasm Metastasis Antibodies  >247 publications with CXCL12 and Neoplasm Metastasis
 Leukemia Antibodies  >212 publications with CXCL12 and Leukemia
 Breast Neoplasms Antibodies  >170 publications with CXCL12 and Breast Neoplasms
 Necrosis Antibodies  >144 publications with CXCL12 and Necrosis
 Myocardial Ischemia Antibodies  >122 publications with CXCL12 and Myocardial Ischemia
 Lymphoma Antibodies  >120 publications with CXCL12 and Lymphoma
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >924 publications with CXCL12 and Bone
 Bone Marrow Antibodies  >855 publications with CXCL12 and Bone Marrow
 Vascular Antibodies  >706 publications with CXCL12 and Vascular
 Brain Antibodies  >316 publications with CXCL12 and Brain
 Muscle Antibodies  >203 publications with CXCL12 and Muscle
 Lung Antibodies  >194 publications with CXCL12 and Lung
 Liver Antibodies  >173 publications with CXCL12 and Liver
 Heart Antibodies  >152 publications with CXCL12 and Heart
 Embryonic Tissue Antibodies  >151 publications with CXCL12 and Embryonic Tissue
 Spleen Antibodies  >104 publications with CXCL12 and Spleen
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