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GP recombinant protein :: Sudan ebolavirus Envelope glycoprotein Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS1368306
SDS-PAGE
Unit / Price
0.01 mg (Yeast)  /  $110 +1 FREE 8GB USB
0.05 mg (Yeast)  /  $190 +1 FREE 8GB USB
0.01 mg (Mammalian-Cell)  /  $205 +1 FREE 8GB USB
0.1 mg (Yeast)  /  $285 +1 FREE 8GB USB
0.02 mg (Mammalian-Cell)  /  $295 +1 FREE 8GB USB
0.2 mg (Yeast)  /  $460 +1 FREE 8GB USB
0.05 mg (Mammalian-Cell)  /  $575 +1 FREE 8GB USB
0.5 mg (Yeast)  /  $750 +1 FREE 8GB USB
0.1 mg (Mammalian-Cell)  /  $795 +1 FREE 8GB USB
1 mg (Yeast)  /  $1,180 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Sudan ebolavirus Envelope glycoprotein (GP), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Sudan ebolavirus Envelope glycoprotein

 Product Synonym Names    GP1,2; GP
 Product Gene Name   

GP recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 MBS1368306 COA    COA PDF
 Sequence Positions    1-482. Full length.
 Sequence    MENKIEVNNK DEMNKWFEEF KKGNGLVDTF TNPYSFCESV PNLERFVFQM ASATDDAQKD SIYASALVEA TKFCAPIYEC AWVSSTGIVK KGLEWFEKNA GTIKSWDESY IELKVEVPKI EQLANYQQAA LKWRKDIGFR VNANTAALSH KVLAEYKVPG EIVMSVKEML SDMIRRRNLI LNRGGDENPR GPVSREHVEW CREFVKGKYI MAFNPPWGDI NKSGRSGIAL VATGLAKLAE TEGKGVFDEA KKTVEALNGY LDKHKDEVDK ASADNMITNL LKHIAKAQEL YKNSSALRAQ GAQIDTAFSS YYWLYKAGVT PETFPTVSQF LFELGKQPRG TKKMKKALLS TPMKWGKKLY ELFADDSFQQ NRIYMHPAVL TAGRISEMGV CFGTIPVANP DDAAQGSGHT KSILNLRTNT ETNNPCAKTI VKLFEIQKTG FNIQDMDIVA SEHLLHQSLV GKQSPFQNAY NVKGNATSAN II
 3D Structure    ModBase 3D Structure for Q7T9D9
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 Host    E Coli or Yeast or Baculovirus or Mammalian Cell
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 Purity/Purification    Greater than 90% as determined by SDS-PAGE. (lot specific)
 Form/Format    Liquid containing glycerol
 Tag Information    This protein contains an N-terminal tag and may also contain a C-terminal Myc-tag. N-terminal host tags may vary (His, His-SUMO, His-B2M, GST). Tag types are determined by various factors including tag-protein stability and, therefore, are subject to change; please inquire for tag information.
 Sterility    Sterile filter available upon request.
 Endotoxin    Low endotoxin available upon request.
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 Preparation and Storage    Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of GP recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for GP recombinant protein

   GP1 is responsible for binding to the receptor(s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. Binding to CD209 and CLEC4M, which are respectively found on dendritic cells (DCs), and on endothelial cells of liver sinusoids and lymph node sinuses, facilitate infection of macrophages and endothelial cells. These interactions not only facilitate virus cell entry, but also allow capture of viral particles by DCs and subsequent transmission to susceptible cells without DCs infection (trans infection). Binding to the macrophage specific lectin CLEC10A also ses to enhance virus infectivity. Interaction with FOLR1/folate receptor alpha may be a cofactor for virus entry in some cell types, although results are contradictory. After internalization of the virus into the endosomes of the host cell, proteolysis of GP1 by two cysteine proteases, CTSB/cathepsin B and CTSL/cathepsin L presumably induces a conformational change of GP2, unmasking its fusion peptide and initiating membranes fusion. GP2 acts as a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in GP2, releasing the fusion hydrophobic peptide. GP1,2 mediates endothelial cell activation and decreases endothelial barrier function. Mediates activation of primary macrophages. At terminal stages of the viral infection, when its expression is high, GP1,2 down-modulates the expression of various host cell surface molecules that are essential for immune surveillance and cell adhesion. Down-modulates integrins ITGA1, ITGA2, ITGA3, ITGA4, ITGA5, ITGA6, ITGAV and ITGB1. GP1,2 alters the cellular recycling of the dimer alpha-V/beta-3 via a dynamin-dependent pathway. Decrease in the host cell surface expression of various adhesion molecules may lead to cell detachment, contributing to the disruption of blood vessel integrity and horrhages developed during Ebola virus infection (cytotoxicity). This cytotoxicity appears late in the infection, only after the massive release of viral particles by infected cells. Down-modulation of host MHC-I, leading to altered recognition by immune cells, may explain the immune suppression and inflammatory dysfunction linked to Ebola infection. Also down-modulates EGFR surface expression. GP2delta is part of the complex GP1,2delta released by host ADAM17 metalloprotease. This secreted complex may play a role in the pathogenesis of the virus by efficiently blocking the neutralizing antibodies that would otherwise neutralize the virus surface glycoproteins GP1,2. Might therefore contribute to the lack of inflammatory reaction seen during infection in spite the of extensive necrosis and massive virus production. GP1,2delta does not se to be involved in activation of primary macrophages.
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 SDS-PAGE of GP recombinant protein    GP recombinant protein SDS-PAGE image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
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NCBI/Uniprot data below describe general gene information for GP. It may not necessarily be applicable to this product.
 NCBI GI #    55770812
 NCBI GeneID    3160774
 NCBI Accession #    YP_138523.1 [Other Products]
 NCBI GenBank Nucleotide #    NC_006432.1 [Other Products]
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 UniProt Primary Accession #    Q7T9D9 [Other Products]
 Molecular Weight    17.42kD
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 NCBI Official Full Name    spike glycoprotein
 NCBI Official Symbol    GP [Similar Products]
 NCBI Protein Information    small secreted glycoprotein; spike glycoprotein
 UniProt Protein Name    Envelope glycoprotein
 UniProt Synonym Protein Names   
GP1,2; GP
 Protein Family    Super small secreted glycoprotein
 UniProt Gene Name    GP [Similar Products]
 UniProt Synonym Gene Names    GP [Similar Products]
 UniProt Entry Name    VGP_EBOSU
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 UniProt Comments for GP    GP1 is responsible for binding to the receptor(s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. Binding to CD209 and CLEC4M, which are respectively found on dendritic cells (DCs), and on endothelial cells of liver sinusoids and lymph node sinuses, facilitate infection of macrophages and endothelial cells. These interactions not only facilitate virus cell entry, but also allow capture of viral particles by DCs and subsequent transmission to susceptible cells without DCs infection (trans infection). Binding to the macrophage specific lectin CLEC10A also seem to enhance virus infectivity. Interaction with FOLR1/folate receptor alpha may be a cofactor for virus entry in some cell types, although results are contradictory. Members of the Tyro3 receptor tyrosine kinase family also seem to be cell entry factors in filovirus infection. Once attached, the virions are internalized through clathrin-dependent endocytosis and/or macropinocytosis. After internalization of the virus into the endosomes of the host cell, proteolysis of GP1 by two cysteine proteases, CTSB/cathepsin B and CTSL/cathepsin L presumably induces a conformational change of GP2, allowing its binding to the host entry receptor NPC1 and unmasking its fusion peptide to initiate membranes fusion.
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Product References and Citations for GP recombinant protein

   Analysis of human peripheral blood samples from fatal and nonfatal cases of Ebola (Sudan) hemorrhagic fever cellular responses, virus load, and nitric oxide levels.Sanchez A., Lukwiya M., Bausch D., Mahanty S., Sanchez A.J., Wagoner K.D., Rollin P.E.J. Virol. 78:10370-10377(2004) Rapid diagnosis of Ebola hemorrhagic fever by reverse transcription-PCR in an outbreak setting and assessment of patient viral load as a predictor of outcome.Towner J.S., Rollin P.E., Bausch D.G., Sanchez A., Crary S.M., Vincent M., Lee W.F., Spiropoulou C.F., Ksiazek T.G., Lukwiya M., Kaducu F., Downing R., Nichol S.T.J. Virol. 78:4330-4341(2004) Complete genome sequence of an Ebola virus (Sudan species) responsible for a 2000 outbreak of human disease in Uganda.Sanchez A., Rollin P.E.Virus Res. 113:16-25(2005)
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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