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CD3e recombinant protein :: T-Cell Surface Glycoprotein CD3 Epsilon (CD3e) Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS2011901 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
0.01 mg  /  $190 +1 FREE 8GB USB
0.05 mg  /  $400 +1 FREE 8GB USB
0.1 mg  /  $620 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

T-Cell Surface Glycoprotein CD3 Epsilon (CD3e), Recombinant Protein

 Also Known As   

Recombinant T-Cell Surface Glycoprotein CD3 Epsilon (CD3e)

 Product Gene Name   

CD3e recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-MGG ITQTPYKVSI SGTTVILTCP QYPGSEILWQ HNDKNIGGDE DDKNIGSDED HLSLKEFSEL EQSGYYVCYP RGSKPEDANF YLYLRARVCE NCMEMDVMSV ATIVIVDICI TGGLLLLVYY WSKNRKAKAK PVTRGAGAGG RQRGQNKERP PPVPN
 Chromosome Location    Chromosome: 11; NC_000011.9 (118175295..118186890). Location: 11q23
 OMIM    186830
 3D Structure    ModBase 3D Structure for P07766
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 Host    E Coli
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 Purity/Purification    > 95%
 Form/Format    Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
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 Organism    Homo sapiens (Human)
 Expression System    Prokaryotic expression
 Residues    Met28~Asn185 (Accession # P07766) with two N-terminal Tags, His-tag and T7-tag
 Endotoxin Level    <1.0EU per 1ug (determined by the LAL method)
 Reconstitution    Reconstitute in sterile PBS, pH7.2-pH7.4.
 Predicted isoelectric point    7.1
 Usage    Reconstitute in ddH2O.
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 Preparation and Storage    Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
 Other Notes    Small volumes of CD3e recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for CD3e recombinant protein

   About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
 Applications Tested/Suitable for CD3e recombinant protein   

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

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 SDS-PAGE of CD3e recombinant protein    CD3e recombinant protein SDS-PAGE image
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 SDS-PAGE of CD3e recombinant protein    [ DESCRIPTION]
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10 kDa, 14 kDa, 18 kDa, 22 kDa, 26 kDa, 33 kDa, 44 kDa and 70 kDa.
Double intensity bands: The 26 kDa, 18 kDa, 10 kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
[ STORAGE BUFFER]
62.5mM Tris-H3P04 (pH 7.5 at 25C), 1 mM EDTA, 2% (w/v) SDS, 100mM DTT, 1 mM NaN3, 0.01 % (w/v) bromo-phenol blue and 33% (v/v) glycerol.
[APPLICATION]
Accurate protein sizing on SDS-polyacrylamide gels and Western blots.
[USAGE]
1. Allow the Marker to reach room temperature and thoroughly mix before use. This will ensure that any solids that may have precipitated at -20C have returned to solution. Do not boil!
2. Load the following volumes of the Marker on a SDS-polyacrylamide gel.
[ LOADING VOLUMES]
3~5uL per well for mini gel; 7uL per well for large gel.
Use the same volumes for Western blotting applications. The loading volumes listed above are recommended for gels with a thickness of 0.75~1.0mm. The loading volume should be double for 1.5mm thick gels.
[ QUALITY CONTROL]
The protein marker, with molecular weight shifts of < or = 5% and minimal band broadening, are confirmed by migration in SDS-PAGE system. Electrophoresis of the Marker on a 13~18% Tris-glycine SDS-polyacrylamide gel resolves 8 individual bands.
[IMPORTANT NOTES]
1. The protein marker is conveniently packaged and ready to use. There is no need to heat, dilute or add reducing agents. Do not boil, which may cause band degradation.
2. The molecular weights of the proteins have a lot-to-Iot variation of ~5%.
3. These protein markers are for SDS-PAGE and should not to be used for native electrophoresis or a native gel. These markers are denatured and have SDS in the storage buffer.
4. Don't load too much protein. See recommended load volumes in the manual.
5. Check % gel that is being used if the lands miss. Depending on gel type and/or percentage, you may not see all of the bands. For example, one would not see the smallest bands of the standard on a very low % gel. A high % gel may not resolve the higher MW bands. Homogeneous low percentage gels are recommended for analysis of large proteins and high percentage gels for analysis of small proteins. In high percentage gels (13~18%) large proteins (22~70kDa) can separate, while in low percentage gels (4~8%) small proteins (14 and 10kDa) will migrate with the tracking dye. Longer transfer times or higher transfer voltages may be required for Western blotting of large (>100kDa) proteins.
6. If additional bands are observed in the gel image of the protein ladder, this might be caused by DTT oxidation in the storage buffer.
7. If proteins show a poor transfer, increase voltage, current or length of time for transfer, and pay attention to the SDS from the transfer buffer. SDS will cause the proteins to bind less efficiently to membranes because it disrupts the hydrophobic interaction between the membrane and the protein. If SDS is present in transfer buffer, make sure that there is no more than 0.02% in buffer.
8. Alcohol enhances hydrophobic binding to membrane. Not enough alcohol may prevent binding.
[ SHELF LIFE]
Storage: -20 degree C, 1 year at -20 degree C. If the product has been stored as directed for one year, its shelf life maybe extended by adding dithiothreitol (Dn) to approximately 50mM.
CD3e recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for CD3e. It may not necessarily be applicable to this product.
 NCBI GI #    4502671
 NCBI GeneID    916
 NCBI Accession #    NP_000724.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000733.3 [Other Products]
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 UniProt Primary Accession #    P07766 [Other Products]
 UniProt Secondary Accession #    A8K997 [Other Products]
 UniProt Related Accession #    P07766 [Other Products]
 Molecular Weight    21.4kDa
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 NCBI Official Full Name    T-cell surface glycoprotein CD3 epsilon chain
 NCBI Official Synonym Full Names    CD3e molecule, epsilon (CD3-TCR complex)
 NCBI Official Symbol    CD3E [Similar Products]
 NCBI Official Synonym Symbols   
T3E; TCRE
[Similar Products]
 NCBI Protein Information    T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell antigen receptor complex, epsilon subunit of T3
 UniProt Protein Name    T-cell surface glycoprotein CD3 epsilon chain
 UniProt Synonym Protein Names   
T-cell surface antigen T3/Leu-4 epsilon chain
 Protein Family    T-cell surface glycoprotein
 UniProt Gene Name    CD3E [Similar Products]
 UniProt Synonym Gene Names    T3E [Similar Products]
 UniProt Entry Name    CD3E_HUMAN
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 NCBI Summary for CD3e    The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]
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 UniProt Comments for CD3e    CD3E: a T cell surface glycoprotein that is a component of the T cell antigen receptor. The recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation. Contains 1 immunoglobulin-like domain and 1 ITAM domain.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: T cell receptor complex; integral to plasma membrane; plasma membrane; immunological synapse; alpha-beta T cell receptor complex; intercellular junction; external side of plasma membrane

Molecular Function: protein binding; receptor signaling complex scaffold activity; transmembrane receptor activity; protein heterodimerization activity; receptor signaling protein activity; T cell receptor binding; protein kinase binding; SH3 domain binding

Biological Process: regulation of immune response; T cell activation; positive regulation of interleukin-2 biosynthetic process; signal complex assembly; positive regulation of calcium-mediated signaling; negative thymic T cell selection; T cell receptor signaling pathway; regulation of apoptosis; positive regulation of interleukin-4 production; G-protein coupled receptor protein signaling pathway; positive regulation of interferon-gamma production; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; negative regulation of smoothened signaling pathway; T cell costimulation; positive regulation of alpha-beta T cell proliferation; positive regulation of T cell proliferation; protein complex assembly; positive regulation of T cell anergy; transmembrane receptor protein tyrosine kinase signaling pathway; response to nutrient

Disease: Immunodeficiency 18
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 Research Articles on CD3e    1. ABCB1 homozygous 3435 TT carrier subjects showed the lowest Pgp activity compared with 3435 CT and CC carriers of renal transplant patients.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CD3e recombinant proteinPathways associated with CD3e recombinant protein
 Reference Product  PubMed Publications
 CD4 recombinant protein  >61 publications with CD3e and CD4
 ZAP70 recombinant protein  >15 publications with CD3e and ZAP70
 LCK recombinant protein  >14 publications with CD3e and LCK
 CD3D recombinant protein  >11 publications with CD3e and CD3D
 CD3G recombinant protein  >6 publications with CD3e and CD3G
 CD8A recombinant protein  >4 publications with CD3e and CD8A
 SYK recombinant protein  >4 publications with CD3e and SYK
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 CXCR4-mediated Signaling Events Pathway antibodies  CXCR4-mediated Signaling Events Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Costimulation By The CD28 Family Pathway antibodies  Costimulation By The CD28 Family Pathway Diagram
 Downstream TCR Signaling Pathway antibodies  Downstream TCR Signaling Pathway Diagram
 Downstream Signaling In Naive CD8+ T Cells Pathway antibodies  Downstream Signaling In Naive CD8+ T Cells Pathway Diagram
 Generation Of Second Messenger Molecules Pathway antibodies  Generation Of Second Messenger Molecules Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
Diseases associated with CD3e recombinant proteinOrgans/Tissues associated with CD3e recombinant protein
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >45 publications with CD3e and Neoplasms
 Lymphoma Antibodies  >26 publications with CD3e and Lymphoma
 Inflammation Antibodies  >17 publications with CD3e and Inflammation
 Immunologic Deficiency Syndromes Antibodies  >13 publications with CD3e and Immunologic Deficiency Syndromes
 Necrosis Antibodies  >12 publications with CD3e and Necrosis
 Nervous System Diseases Antibodies  >12 publications with CD3e and Nervous System Diseases
 Neoplasms, Experimental Antibodies  >7 publications with CD3e and Neoplasms, Experimental
 Lung Diseases Antibodies  >5 publications with CD3e and Lung Diseases
 Carcinoma Antibodies  >5 publications with CD3e and Carcinoma
 Arthritis, Rheumatoid Antibodies  >5 publications with CD3e and Arthritis, Rheumatoid
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >179 publications with CD3e and Blood
 Thymus Antibodies  >44 publications with CD3e and Thymus
 Spleen Antibodies  >31 publications with CD3e and Spleen
 Lymph Node Antibodies  >15 publications with CD3e and Lymph Node
 Liver Antibodies  >11 publications with CD3e and Liver
 Intestine Antibodies  >10 publications with CD3e and Intestine
 Brain Antibodies  >6 publications with CD3e and Brain
 Lung Antibodies  >6 publications with CD3e and Lung
 Kidney Antibodies  >6 publications with CD3e and Kidney
 Eye Antibodies  >4 publications with CD3e and Eye
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