Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Recombinant Protein rightarrow TIFA  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Ajuba Antibody
 • ARSB - Arylsulfatase B Antibody
 • Beta-lactamase (ampC) Recombinant Protein
 • PKC-epsilon Protein
 • GRPEL2 Antibody
 • Soluble Intercellular Adhesion Molecule-1 (SICAM1) ELISA Kit
 • leucine-rich, glioma inactivated 1 (LGI1) ELISA Kit
 • Interferon Gamma Induced Protein 10kDa (IP10) ELISA Kit
 • GABA A Receptor beta 2,3 (GABRB2) Antibody
 • HAS2 Antibody
 • KIRREL Antibody
 • 60S ribosomal protein L11 (RPL11) Recombinant Protein
 • H5 (H5N1) (A-Anhui-1-2005) Recombinant Protein
 • CD79B Recombinant Protein
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

TIFA recombinant protein :: TRAF-interacting protein with FHA domain-containing protein A Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS204665
SDS-PAGE
Unit / Price
0.05 mg  /  $375 +1 FREE 8GB USB
0.25 mg  /  $925 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

TRAF-interacting protein with FHA domain-containing protein A (TIFA), Recombinant Protein

★Popular Item★
 Also Known As   

TIFA, 1-184aa Human, His-tagged, Recombiannt, E Coli

 Product Synonym Names    T2BP; T6BP; TIFAA
 Product Gene Name   

TIFA recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 Sequence    MGSSHHHHHH SSGLVPRGSH MGSHMTSFED ADTEETVTCL QMTVYHPGQL QCGIFQSISF NREKLPSSEV VKFGRNSNIC HYTFQDKQVS RVQFSLQLFK KFNSSVLSFE IKNMSKKTNL IVDSRELGYL NKMDLPYRCM VRFGEYQFLM EKEDGESLEF FETQFILSPR SLLQENNWPP HRPIPEYGTY SLCSSQSSSP TEMDENES
Section Bar
 Host    E.coli
 Species Reactivity    Human
Section Bar
 Purity/Purification    > 90% by SDS - PAGE
 Form/Format    Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT
 Concentration    0.5 mg/ml (determined by Bradford assay) (lot specific)
Section Bar
 Tag    His-tag
Section Bar
 Preparation and Storage    Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of TIFA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 

Product Description specifically for TIFA recombinant protein

   TIFA, also known as TRAF-interacting protein with FHA domain-containing protein A, is an adapter protein which mediates the IRAK1 and TRAF6 interaction following IL-1 stimulation, resulting in the downstream activation of NF-kappa-B and AP-1 pathways. This protein induces the oligomerization and polyubiquitination of TRAF6, which leads to the activation of TAK1 and IKK through a proteasome-independent mechanism. Recombinant human TIFA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
 Product Categories/Family for TIFA recombinant protein    Signal Transduction
 Applications Tested/Suitable for TIFA recombinant protein   

SDS-PAGE

Section Bar
 SDS-PAGE of TIFA recombinant protein    TIFA recombinant protein SDS-PAGE image
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for TIFA. It may not necessarily be applicable to this product.
 NCBI GI #    33563370
 NCBI GeneID    92610
 NCBI Accession #    NP_443096 [Other Products]
 NCBI GenBank Nucleotide #    NM_052864.2 [Other Products]
Section Bar
 UniProt Related Accession #    Q96CG3 [Other Products]
 Molecular Weight    24.0 kDa (208aa) confirmed by MALDI-TOF
Table BarTOPTable Bar
 NCBI Official Full Name    TRAF-interacting protein with FHA domain-containing protein A
 NCBI Official Synonym Full Names    TRAF-interacting protein with forkhead-associated domain
 NCBI Official Symbol    TIFA [Similar Products]
 NCBI Official Synonym Symbols   
T2BP; T6BP; TIFAA
[Similar Products]
 NCBI Protein Information    TRAF-interacting protein with FHA domain-containing protein A; TRAF-interacting protein with a forkhead-associated domain; TRAF2 binding protein; TRAF2-binding protein; TRAF6 binding protein; putative MAPK-activating protein PM14; putative NF-kappa-B-activating protein 20
 UniProt Protein Name    TRAF-interacting protein with FHA domain-containing protein A
 UniProt Synonym Protein Names   
Putative MAPK-activating protein PM14; Putative NF-kappa-B-activating protein 20; TRAF2-binding protein
 Protein Family    TRAF-interacting protein with FHA domain-containing protein
 UniProt Gene Name    TIFA [Similar Products]
 UniProt Synonym Gene Names    T2BP [Similar Products]
 UniProt Entry Name    TIFA_HUMAN
Section Bar
 UniProt Comments for TIFA    TIFA: Adapter protein which mediates the IRAK1 and TRAF6 interaction following IL-1 stimulation, resulting in the downstream activation of NF-kappa-B and AP-1 pathways. Induces the oligomerization and polyubiquitination of TRAF6, which leads to the activation of TAK1 and IKK through a proteasome-independent mechanism.

Chromosomal Location of Human Ortholog: 4q25

Molecular Function: protein binding

Biological Process: I-kappaB kinase/NF-kappaB cascade
Table BarTOPTable Bar
 

Product References and Citations for TIFA recombinant protein

   Takatsuna H., et al. (2003) J. Biol. Chem. 278:12144-12150
Ea C.-K., et al. (2004) Proc. Natl. Acad. Sci. u.S.A. 101:15318-15323
Section Bar
 Research Articles on TIFA    1. identify a novel threonine phosphorylation site on TIFA and show that this phosphorylated threonine binds with the FHA domain of TIFA, leading to TIFA oligomerization and TIFA-mediated NF-kappaB activation
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with TIFA recombinant proteinPathways associated with TIFA recombinant protein
 Reference Product  PubMed Publications
 TRAF6 recombinant protein  >7 publications with TIFA and TRAF6
 TIFAB recombinant protein  >3 publications with TIFA and TIFAB
 ZCCHC11 recombinant protein  >1 publications with TIFA and ZCCHC11
 Products by Pathway  Pathway Diagram
 TNF-alpha/NF-kB Signaling Pathway antibodies  TNF-alpha/NF-kB Signaling Pathway Diagram
Diseases associated with TIFA recombinant proteinOrgans/Tissues associated with TIFA recombinant protein
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >8 publications with TIFA and Necrosis
 Musculoskeletal Diseases Antibodies  >3 publications with TIFA and Musculoskeletal Diseases
 Inflammation Antibodies  >2 publications with TIFA and Inflammation
 Abnormalities, Multiple Antibodies  >1 publications with TIFA and Abnormalities, Multiple
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >5 publications with TIFA and Bone
 Blood Antibodies  >3 publications with TIFA and Blood
 Spleen Antibodies  >1 publications with TIFA and Spleen
 Bone Marrow Antibodies  >1 publications with TIFA and Bone Marrow
 Liver Antibodies  >1 publications with TIFA and Liver
 Eye Antibodies  >1 publications with TIFA and Eye
 Lymph Antibodies  >1 publications with TIFA and Lymph
 Kidney Antibodies  >1 publications with TIFA and Kidney
 Skin Antibodies  >1 publications with TIFA and Skin
 Thymus Antibodies  >1 publications with TIFA and Thymus
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions