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TTK recombinant protein :: TTK Protein Kinase Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS2030957 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
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Unit / Price
0.01 mg  /  $145 +1 FREE 8GB USB
0.05 mg  /  $270 +1 FREE 8GB USB
0.1 mg  /  $420 +1 FREE 8GB USB
0.2 mg  /  $515 +1 FREE 8GB USB
0.5 mg  /  $995 +1 FREE 8GB USB
1 mg  /  $1,480 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

TTK Protein Kinase, Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant TTK Protein Kinase (TTK)

 Product Gene Name   

TTK recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHH H SSGLVPRGSH MASMTGGQQM GRGSEF- LKQI GSGGSSKVFQ VLNEKKQINA IKYVNLEDAD SQTIESYRNE IAFLNKLQQH SDKIIRLYDY EITEQYIYMV MECGNIDLNS WLKKKKSINP WERKSYWKNM LEAVHIIHQH GIVHSDLKPA NFVIVDGMLK LIDFGIANQM QPDTTSIVKD SQVGTVNYMA PEAIRDMSSS RENSKIRTKV SPRSDVWSLG CILYYMTYGR TPFQHIINQV SKLHAIINPA HEIEFPEISE KDLRDVLKCC LVRNPKERIS IPELLT
 OMIM    604092
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 Host    Host: E Coli
Source: Prokaryotic expression
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 Purity/Purification    >95%
 Form/Format    Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
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 Predicted Molecular Mass    33.9kDa
 Accurate Molecular Mass (KD)    35/25 kDa
 Endotoxin    <1.0EU per 1ug (determined by the LAL method)
 Expression System    Prokaryotic expression
 Tag    N-terminal His-Tag, T7 tag
 Organism Species    Mus musculus (Mouse)
 Fragment    Leu527~Thr786
 Usage    Reconstitute in sterile PBS, pH7.2-pH7.4.
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 Preparation and Storage    Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
 Other Notes    Small volumes of TTK recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for TTK recombinant protein   

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

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 SDS-Page of TTK recombinant protein    TTK recombinant protein SDS-Page image
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NCBI/Uniprot data below describe general gene information for TTK. It may not necessarily be applicable to this product.
 NCBI GI #    23271249
 NCBI GeneID    7272
 NCBI Accession #    AAH32858.1 [Other Products]
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 UniProt Secondary Accession #    Q15272; Q5TCS0; Q9BW51; Q9NTM0; A8K8U5; B2RDW2; E1P543 [Other Products]
 UniProt Related Accession #    P33981 [Other Products]
 Molecular Weight    96,944 Da
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 NCBI Official Full Name    TTK protein kinase
 NCBI Official Synonym Full Names    TTK protein kinase
 NCBI Official Symbol    TTK [Similar Products]
 NCBI Official Synonym Symbols   
ESK; PYT; CT96; MPH1; MPS1; MPS1L1
[Similar Products]
 NCBI Protein Information    dual specificity protein kinase TTK
 UniProt Protein Name    Dual specificity protein kinase TTK
 UniProt Synonym Protein Names   
Phosphotyrosine picked threonine-protein kinase; PYT
 Protein Family    Protein tramtrack
 UniProt Gene Name    TTK [Similar Products]
 UniProt Synonym Gene Names    MPS1; MPS1L1; PYT [Similar Products]
 UniProt Entry Name    TTK_HUMAN
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 NCBI Summary for TTK    This gene encodes a dual specificity protein kinase with the ability to phosphorylate tyrosine, serine and threonine. Associated with cell proliferation, this protein is essential for chromosome alignment at the centromere during mitosis and is required for centrosome duplication. It has been found to be a critical mitotic checkpoint protein for accurate segregation of chromosomes during mitosis. Tumorigenesis may occur when this protein fails to degrade and produces excess centrosomes resulting in aberrant mitotic spindles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2009]
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 UniProt Comments for TTK    TTK: a dual-specificity protein kinase orthologous to the yeast monopolar spindle (Mps1) kinase that is required for spindle pole duplication and the spindle checkpoint but not for centrosome duplication. Localized to centrosomes, kinetochores and nuclear pores. Its enzymatic activity is required for the recruitment of Mad1, Mad2, Bub1 and Bub3 to the kinetochore. Mps1 mRNA levels change little throughout the cell cycle, but its protein expression varies during the cell cycle. Overexpression arrests cells in metaphase by activating the checkpoint.

Protein type: EC 2.7.12.1; Protein kinase, Other; Kinase, protein; Cancer Testis Antigen (CTA); Protein kinase, dual-specificity (non-receptor); Other group; TTK family

Chromosomal Location of Human Ortholog: 6q14.1

Cellular Component: membrane; spindle

Molecular Function: protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; ATP binding

Biological Process: mitotic spindle organization and biogenesis; peptidyl-tyrosine phosphorylation; positive regulation of cell proliferation; mitotic cell cycle spindle assembly checkpoint; spindle organization and biogenesis; chromosome separation
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 Research Articles on TTK    1. TTK was up-regulated in HCC specimens. TTK overexpression promoted cell proliferation, anchor-dependent colony formation and resistance to sorafenib of HCC cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TTK recombinant proteinPathways associated with TTK recombinant protein
 Reference Product  PubMed Publications
 BUB1 recombinant protein  >25 publications with TTK and BUB1
 MAD2L1 recombinant protein  >19 publications with TTK and MAD2L1
 MAD1L1 recombinant protein  >12 publications with TTK and MAD1L1
 CDC20 recombinant protein  >9 publications with TTK and CDC20
 NDC80 recombinant protein  >9 publications with TTK and NDC80
 CDK2 recombinant protein  >8 publications with TTK and CDK2
 NUF2 recombinant protein  >5 publications with TTK and NUF2
 CDC27 recombinant protein  >1 publications with TTK and CDC27
 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 RB In Cancer Pathway antibodies  RB In Cancer Pathway Diagram
Diseases associated with TTK recombinant proteinOrgans/Tissues associated with TTK recombinant protein
 Disease Name  Pubmed Publications
 Brain Diseases Antibodies  >19 publications with TTK and Brain Diseases
 Breast Neoplasms Antibodies  >16 publications with TTK and Breast Neoplasms
 Disease Models, Animal Antibodies  >12 publications with TTK and Disease Models, Animal
 Lung Neoplasms Antibodies  >8 publications with TTK and Lung Neoplasms
 Carcinoma, Hepatocellular Antibodies  >8 publications with TTK and Carcinoma, Hepatocellular
 Neoplasms, Experimental Antibodies  >7 publications with TTK and Neoplasms, Experimental
 Lung Diseases Antibodies  >7 publications with TTK and Lung Diseases
 Liver Diseases Antibodies  >6 publications with TTK and Liver Diseases
 Cell Transformation, Neoplastic Antibodies  >6 publications with TTK and Cell Transformation, Neoplastic
 Liver Neoplasms Antibodies  >5 publications with TTK and Liver Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >58 publications with TTK and Brain
 Eye Antibodies  >40 publications with TTK and Eye
 Blood Antibodies  >38 publications with TTK and Blood
 Muscle Antibodies  >17 publications with TTK and Muscle
 Heart Antibodies  >15 publications with TTK and Heart
 Embryonic Tissue Antibodies  >13 publications with TTK and Embryonic Tissue
 Liver Antibodies  >12 publications with TTK and Liver
 Testis Antibodies  >11 publications with TTK and Testis
 Bone Antibodies  >9 publications with TTK and Bone
 Vascular Antibodies  >8 publications with TTK and Vascular
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