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MAPKAP1 recombinant protein :: Target of rapamycin complex 2 subunit MAPKAP1 Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS1364264
SDS-Page
Unit / Price
0.05 mg (E-Coli)  /  $170 +1 FREE 8GB USB
0.2 mg (E-Coli)  /  $395 +1 FREE 8GB USB
0.5 mg (E-Coli)  /  $640 +1 FREE 8GB USB
1 mg (E-Coli)  /  $1,000 +1 FREE 8GB USB
0.05 mg (Baculovirus)  /  $1,310 +1 FREE 8GB USB
0.5 mg (Yeast)  /  $1,530 +1 FREE 8GB USB
0.05 mg (Mammalian-Cell)  /  $1,530 +1 FREE 8GB USB
0.1 mg (Baculovirus)  /  $2,160 +2 FREE 8GB USB
1 mg (Yeast)  /  $2,625 +3 FREE 8GB USB
0.1 mg (Mammalian-Cell)  /  $2,625 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Target of rapamycin complex 2 subunit MAPKAP1, Recombinant Protein

 Also Known As   

Recombinant Human Target of rapamycin complex 2 subunit MAPKAP1

 Product Synonym Names    Mitogen-activated protein kinase 2-associated protein 1; Stress-activated map kinase-interacting protein 1; SAPK-interacting protein 1; mSIN1
 Product Gene Name   

MAPKAP1 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence Positions    2-522
 Sequence    AFLDNPTIIL AHIRQSHVTS DDTGMCEMVL IDHDVDLEKI HPPSMPGDSG SEIQGSNGET QGYVYAQSVD ITSSWDFGIR RRSNTAQRLE RLRKERQNQI KCKNIQWKER NSKQSAQELK SLFEKKSLKE KPPISGKQSI LSVRLEQCPL QLNNPFNEYS KFDGKGHVGT TATKKIDVYL PLHSSQDRLL PMTVVTMASA RVQDLIGLIC WQYTSEGREP KLNDNVSAYC LHIAEDDGEV DTDFPPLDSN EPIHKFGFST LALVEKYSSP GLTSKESLFV RINAAHGFSL IQVDNTKVTM KEILLKAVKR RKGSQKVSGP QYRLEKQSEP NVAVDLDSTL ESQSAWEFCL VRENSSRADG VFEEDSQIDI ATVQDMLSSH HYKSFKVSMI HRLRFTTDVQ LGISGDKVEI DPVTNQKAST KFWIKQKPIS IDSDLLCACD LAEEKSPSHA IFKLTYLSNH DYKHLYFESD AATVNEIVLK VNYILESRAS TARADYFAQK QRKLNRRTSF SFQKEKKSGQ Q
 OMIM    610558
 3D Structure    ModBase 3D Structure for Q9BPZ7
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 Host    E Coli or Yeast or Baculovirus or Mammalian Cell
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 Purity/Purification    Greater than 90% as determined by SDS-PAGE.
 Form/Format    Liquid containing glycerol; lyophilization may be available upon request.
 Tag Information    This protein contains an N-terminal tag and may also contain a C-terminal Myc-tag. N-terminal host tags may vary (His, His-SUMO, His-B2M, GST). Tag types are determined by various factors including tag-protein stability and, therefore, are subject to change; please inquire for tag information.
 Sterility    Sterile filter available upon request.
 Endotoxin    Low endotoxin available upon request.
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 Species    Homo sapiens (Human)
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 Preparation and Storage    Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of MAPKAP1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for MAPKAP1 recombinant protein

   Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Involved in ciliogenesis, regulates cilia length through its interaction with CCDC28B independently of mTORC2 complex.
 Product Categories/Family for MAPKAP1 recombinant protein    Signal Transduction
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 SDS-Page of MAPKAP1 recombinant protein    MAPKAP1 recombinant protein SDS-Page image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
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NCBI/Uniprot data below describe general gene information for MAPKAP1. It may not necessarily be applicable to this product.
 NCBI GI #    56788407
 NCBI GeneID    79109
 NCBI Accession #    NP_001006618.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001006617.1 [Other Products]
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 UniProt Primary Accession #    Q9BPZ7 [Other Products]
 UniProt Secondary Accession #    Q00426; Q5JSV5; Q5JSV6; Q5JSV9; Q658R0; Q699U1; Q699U2; Q699U3; A8K1Z5; B1AMA4; B7Z309 [Other Products]
 UniProt Related Accession #    Q9BPZ7 [Other Products]
 Molecular Weight    74.95kD
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 NCBI Official Full Name    target of rapamycin complex 2 subunit MAPKAP1 isoform 1
 NCBI Official Synonym Full Names    mitogen-activated protein kinase associated protein 1
 NCBI Official Symbol    MAPKAP1 [Similar Products]
 NCBI Official Synonym Symbols   
MIP1; SIN1; JC310; SIN1b; SIN1g
[Similar Products]
 NCBI Protein Information    target of rapamycin complex 2 subunit MAPKAP1
 UniProt Protein Name    Target of rapamycin complex 2 subunit MAPKAP1
 UniProt Synonym Protein Names   
Mitogen-activated protein kinase 2-associated protein 1; Stress-activated map kinase-interacting protein 1; SAPK-interacting protein 1; mSIN1
 Protein Family    Target of rapamycin complex
 UniProt Gene Name    MAPKAP1 [Similar Products]
 UniProt Synonym Gene Names    MIP1; SIN1; TORC2 subunit MAPKAP1; SAPK-interacting protein 1; mSIN1 [Similar Products]
 UniProt Entry Name    SIN1_HUMAN
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 NCBI Summary for MAPKAP1    This gene encodes a protein that is highly similar to the yeast SIN1 protein, a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for MAPKAP1    Sin1: a member of a conserved family of proteins that have an essential roles in signal transduction from yeast to mammals. An essential component of the mTORC2 complex, which consists of mTOR, LST8, Sin1, Protor (PRR5), and RICTOR, the Rapamycin Insensitive Companion of mTOR. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 regulates the phosphorylation of SGK1 S422 and PKCA S657. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Contains a Ras-binding (RBD) and a pleckstrin homology (PH) domains. Plays an important role in the SAPK signaling pathway by binding directly to both ATF-2 and p38. A genetic knockout of Sin1 abolished Akt-S473 phosphorylation and disrupts the RICTOR-mTOR interaction, but Akt-Thr308 phosphorylation is maintained. Six alternatively-spliced isoforms of the human protein have been reported. All isoforms except 4 can be incorporated into the the mTORC2 complex.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 9q33.3

Cellular Component: cytoplasm; cytoplasmic membrane-bound vesicle; cytosol; Golgi apparatus; nucleoplasm; nucleus; plasma membrane; TORC2 complex

Molecular Function: phosphatidylinositol-3,4,5-triphosphate binding; phosphatidylinositol-3,4-bisphosphate binding; phosphatidylinositol-4,5-bisphosphate binding; protein binding; protein kinase binding; Ras GTPase binding

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; innate immune response; negative regulation of Ras protein signal transduction; nerve growth factor receptor signaling pathway; phosphoinositide-mediated signaling; substantia nigra development; T cell costimulation; vascular endothelial growth factor receptor signaling pathway
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Product References and Citations for MAPKAP1 recombinant protein

   "Alternative polyadenylation and splicing of mRNAs transcribed from the human Sin1 gene." Schroder W., Cloonan N., Bushell G., Sculley T. Gene 339:17-23(2004)
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 Research Articles on MAPKAP1    1. MAPKAP1 may represent a novel anti-infection and anti-fibrogenesis genomic locus in chronic schistosomiasis japonica.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with MAPKAP1 recombinant proteinPathways associated with MAPKAP1 recombinant protein
 Reference Product  PubMed Publications
 MTOR recombinant protein  >28 publications with MAPKAP1 and MTOR
 RICTOR recombinant protein  >17 publications with MAPKAP1 and RICTOR
 MLST8 recombinant protein  >6 publications with MAPKAP1 and MLST8
 SGK1 recombinant protein  >1 publications with MAPKAP1 and SGK1
 EIF4EBP1 recombinant protein  >1 publications with MAPKAP1 and EIF4EBP1
 AKT1S1 recombinant protein  >1 publications with MAPKAP1 and AKT1S1
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 CD28 Co-stimulation Pathway antibodies  CD28 Co-stimulation Pathway Diagram
 CD28 Dependent PI3K/Akt Signaling Pathway antibodies  CD28 Dependent PI3K/Akt Signaling Pathway Diagram
 CXCR3-mediated Signaling Events Pathway antibodies  CXCR3-mediated Signaling Events Pathway Diagram
 CXCR4-mediated Signaling Events Pathway antibodies  CXCR4-mediated Signaling Events Pathway Diagram
 Class I PI3K Signaling Events Mediated By Akt Pathway antibodies  Class I PI3K Signaling Events Mediated By Akt Pathway Diagram
 Constitutive Signaling By AKT1 E17K In Cancer Pathway antibodies  Constitutive Signaling By AKT1 E17K In Cancer Pathway Diagram
 Costimulation By The CD28 Family Pathway antibodies  Costimulation By The CD28 Family Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
Diseases associated with MAPKAP1 recombinant proteinOrgans/Tissues associated with MAPKAP1 recombinant protein
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >7 publications with MAPKAP1 and Necrosis
 Inflammation Antibodies  >5 publications with MAPKAP1 and Inflammation
 Disease Models, Animal Antibodies  >3 publications with MAPKAP1 and Disease Models, Animal
 Carcinoma, Hepatocellular Antibodies  >3 publications with MAPKAP1 and Carcinoma, Hepatocellular
 Breast Neoplasms Antibodies  >2 publications with MAPKAP1 and Breast Neoplasms
 Neoplasms, Experimental Antibodies  >2 publications with MAPKAP1 and Neoplasms, Experimental
 Infertility, Male Antibodies  >1 publications with MAPKAP1 and Infertility, Male
 Hemorrhage Antibodies  >1 publications with MAPKAP1 and Hemorrhage
 Neurobehavioral Manifestations Antibodies  >1 publications with MAPKAP1 and Neurobehavioral Manifestations
 Kidney Diseases Antibodies  >1 publications with MAPKAP1 and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >13 publications with MAPKAP1 and Blood
 Liver Antibodies  >4 publications with MAPKAP1 and Liver
 Kidney Antibodies  >3 publications with MAPKAP1 and Kidney
 Lung Antibodies  >3 publications with MAPKAP1 and Lung
 Uterus Antibodies  >2 publications with MAPKAP1 and Uterus
 Embryonic Tissue Antibodies  >2 publications with MAPKAP1 and Embryonic Tissue
 Skin Antibodies  >2 publications with MAPKAP1 and Skin
 Brain Antibodies  >2 publications with MAPKAP1 and Brain
 Prostate Antibodies  >1 publications with MAPKAP1 and Prostate
 Eye Antibodies  >1 publications with MAPKAP1 and Eye
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