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pol1 recombinant protein :: Thermus aquacicus DNA polymerase I, thermostable Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS1265446
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Unit / Price
0.05 mg (E-Coli)  /  $190 +1 FREE 8GB USB
0.2 mg (E-Coli)  /  $460 +1 FREE 8GB USB
0.5 mg (E-Coli)  /  $750 +1 FREE 8GB USB
1 mg (E-Coli)  /  $1,180 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Thermus aquacicus DNA polymerase I, thermostable (pol1), Recombinant Protein

 Also Known As   

Recombinant thermus aquacicus DNA polymerase I, thermostable

 Product Synonym Names    Taq polymerase 1
 Product Gene Name   

pol1 recombinant protein

[Similar Products]
 Product Synonym Gene Name    polA [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence Positions    289-832
 Sequence    ESPKALEEAP WPPPEGAFVG FVLSRKEPMW ADLLALAAAR GGRVHRAPEP YKALRDLKEA RGLLAKDLSV LALREGLGLP PGDDPMLLAY LLDPSNTTPE GVARRYGGEW TEEAGERAAL SERLFANLWG RLEGEERLLW LYREVERPLS AVLAHMEATG VRLDVAYLRA LSLEVAEEIA RLEAEVFRLA GHPFNLNSRD QLERVLFDEL GLPAIGKTEK TGKRSTSAAV LEALREAHPI VEKILQYREL TKLKS
 3D Structure    ModBase 3D Structure for P19821
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 Host    E Coli
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 Purity/Purification    Greater than 90% as determined by SDS-PAGE.
 Tag Information    This protein contains an N-terminal tag and may also contain a C-terminal Myc-tag. N-terminal host tags may vary (His, His-SUMO, His-B2M, GST). Tag types are determined by various factors including tag-protein stability and, therefore, are subject to change; please inquire for tag information. Additional charge for Tag removal. Tag removal service standardly refers to removal of N-terminal Tag. Any C-terminal Myc-tag may remain intact unless removal of the C-terminal Myc-tag is specifically requested. In most cases tags can be successfully removed. If the lab concludes the protein is no longer stable after tag removal then your protein will be supplied with the tag intact, and you will not be charged for tag removal service.
 Sterility    Sterile filter available upon request.
 Endotoxin    Low endotoxin available upon request.
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 Preparation and Storage    Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of pol1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 SDS-Page of pol1 recombinant protein    pol1 recombinant protein SDS-Page image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
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NCBI/Uniprot data below describe general gene information for pol1. It may not necessarily be applicable to this product.
 NCBI GI #    118828
 NCBI Accession #    P19821.1 [Other Products]
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 UniProt Primary Accession #    P19821 [Other Products]
 Molecular Weight    88.7kD
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 NCBI Official Full Name    DNA polymerase I, thermostable
 UniProt Protein Name    DNA polymerase I, thermostable
 UniProt Synonym Protein Names   
Taq polymerase 1
 Protein Family    DNA polymerase
 UniProt Gene Name    polA [Similar Products]
 UniProt Synonym Gene Names    pol1 [Similar Products]
 UniProt Entry Name    DPO1_THEAQ
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Product References and Citations for pol1 recombinant protein

   Isolation, characterization, and expression in Escherichia coli of the DNA polymerase gene from Thermus aquaticus.Lawyer F.C., Stoffel S., Saiki R.K., Myambo K., Drummond R., Gelfand D.H.J. Biol. Chem. 264:6427-6437(1989) Overproduction of Thermus aquaticus DNA polymerase and its structural analysis by ion-spray mass spectrometry.Ishino Y., Ueno T., Miyagi M., Uemori T., Imamura M., Tsunasawa S., Kato I.J. Biochem. 116:1019-1024(1994) Crystal structure of Thermus aquaticus DNA polymerase.Kim Y., Eom S.H., Wang J., Lee D.-S., Suh S.W., Steitz T.A.Nature 376:612-616(1995) Crystal structure of the large fragment of Thermus aquaticus DNA polymerase I at 2.5-A resolution structural basis for thermostability.Korolev S., Nayal M., Barnes W.M., di Cera E., Waksman G.Proc. Natl. Acad. Sci. U.S.A. 92:9264-9268(1995) Structure of Taq polymerase with DNA at the polymerase active site.Eom S.H., Wang J., Steitz T.A.Nature 382:278-281(1996) Crystal structures of open and closed forms of binary and ternary complexes of the large fragment of Thermus aquaticus DNA polymerase I structural basis for nucleotide incorporation.Li Y., Korolev S., Waksman G.EMBO J. 17:7514-7525(1998) Crystal structures of the Klenow fragment of Thermus aquaticus DNA polymerase I complexed with deoxyribonucleoside triphosphates.Li Y., Kong Y., Korolev S., Waksman G.Protein Sci. 7:1116-1123(1998) Structure-based design of Taq DNA polymerases with improved properties of dideoxynucleotide incorporation.Li Y., Mitaxov V., Waksman G.Proc. Natl. Acad. Sci. U.S.A. 96:9491-9496(1999)
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with pol1 recombinant protein
 Reference Product  PubMed Publications
 dnaE recombinant protein  >13 publications with pol1 and dnaE
 mutS recombinant protein  >13 publications with pol1 and mutS
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