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GPHA2 recombinant protein :: Thyrostimulin Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS142652 GPHA2 recombinant protein
Unit / Price
0.002 mg  /  $140 +1 FREE 8GB USB
0.01 mg  /  $205 +1 FREE 8GB USB
1 mg  /  $5,230 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Thyrostimulin (GPHA2), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Human Thyrostimulin Alpha

 Product Synonym Names    GPHA2 Human; Thyrostimulin Alpha Human Recombinant; GPA2; GPHA2; ZSIG51; Glycoprotein hormone alpha-2; MGC126572
 Product Gene Name   

GPHA2 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MRGSHHH HHH GMAS QEAVIPG CHLHPFNVTV RSDRQGTCQG SHVAQACVGH CESSAFPSRY SVLVASGYRH NITSVSQCCT ISGLKKVKVQ LQCVGSRREE LEIFTARACQ CDMCRLSRY
 OMIM    609651
 3D Structure    ModBase 3D Structure for Q96T91
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 Host    E Coli
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 Purity/Purification    Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
 Form/Format    GPHA2 filtered (0.4 um) and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH 4.
Filtered White lyophilized (freeze-dried) powder.
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 Solubility    It is recommended to add 0.1M Acetate buffer pH4 to prepare a working stock solution of ~ 0.5 mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10ug/ml. In higher concentrations the solubility of this protein is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
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 Preparation and Storage    Lyophilized Thyrostimulin although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution GPHA2 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
 Other Notes    Small volumes of GPHA2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for GPHA2 recombinant protein

   Description: GPHA2 Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 120 amino acids and having a total molecular mass of 13.28 kDa. The Thyrostimulin contains His tag which consists of 14 additional amino acids.The amino acid sequence of the recombinant human Thyrostimulin beta subunit is 100% homologous to the amino acid sequence of the human Thyrostimulin beta subunit without signal sequence. (N-terminal 24AA).Thyrostimulin is purified by proprietary chromatographic techniques.

Introduction: Human thyrostimulin ranks among the glycoprotein hormone family. These hormones consist of two subunits, the common alpha- and specific beta-subunits, which associate noncovalently to form a heterodimer. The alpha-subunit combines with four distinct beta-subunits giving rise to four biologically active hormones in human: FSH, LH, TSH, and CG. FSH, LH, and TSH, mainly expressed in the anterior pituitary, are essential for coordinated endocrine regulation in the hypothalamus- pituitary axis and show to activate specific G proteincoupled receptors in the thyroid (TSH receptor) and gonads (LH and FSH receptors), respectively.The heterodimeric glycoprotein hormones have only been identified in vertebrates and are highly conserved in organisms from primitive rayfin fish (Chondrostei) to human in both primary sequences and functional characteristics.Corticotroph-derived glycoprotein hormone (CGH), also referred to as thyrostimulin, is a noncovalent heterodimer of glycoprotein hormone alpha 2 (GPHA2) and glycoprotein hormone beta 5 (GPHB5).Recombinant A2/B5 heterodimeric glycoproteins activates human TSH receptors, but not LH and FSH receptors, and shows high affinity to TSH receptors in a radioligand receptor assay. The heterodimer also stimulates cAMP production and thymidine incorporation by cultured thyroid cells and increases serum thyroxine levels in TSH-suppressed rats in vivo. This new heterodimeric glycoprotein hormone was named as thyrostimulin based on its thyroid-stimulating activity. The expression of thyrostimulin in the anterior pituitary known to express TSH receptors suggested a paracrine mechanism.
 Product Categories/Family for GPHA2 recombinant protein    HORMONES; Hormones; Thyrostimulin
NCBI/Uniprot data below describe general gene information for GPHA2. It may not necessarily be applicable to this product.
 NCBI GI #    18640742
 NCBI GeneID    170589
 NCBI Accession #    NP_570125.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_130769.3 [Other Products]
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 UniProt Primary Accession #    Q96T91 [Other Products]
 UniProt Secondary Accession #    Q52LE2 [Other Products]
 UniProt Related Accession #    Q96T91 [Other Products]
 Molecular Weight    14,163 Da
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 NCBI Official Full Name    glycoprotein hormone alpha-2
 NCBI Official Synonym Full Names    glycoprotein hormone alpha 2
 NCBI Official Symbol    GPHA2 [Similar Products]
 NCBI Official Synonym Symbols   
A2; GPA2; ZSIG51
[Similar Products]
 NCBI Protein Information    glycoprotein hormone alpha-2; cysteine knot protein; glycoprotein alpha 2; putative secreted protein Zsig51; thyrostimulin subunit alpha
 UniProt Protein Name    Glycoprotein hormone alpha-2
 UniProt Synonym Protein Names   
Putative secreted protein Zsig51; Thyrostimulin subunit alpha
 Protein Family    Glycoprotein hormone
 UniProt Gene Name    GPHA2 [Similar Products]
 UniProt Synonym Gene Names    GPA2; ZSIG51 [Similar Products]
 UniProt Entry Name    GPHA2_HUMAN
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 NCBI Summary for GPHA2    GPHA2 is a cystine knot-forming polypeptide and a subunit of the dimeric glycoprotein hormone family (Hsu et al., 2002 [PubMed 12089349]).[supplied by OMIM, Mar 2008]
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 UniProt Comments for GPHA2    GPHA2: Stimulates the thyroid. Binds and activates THSR, leads to increased cAMP production. Belongs to the glycoprotein hormones subunit alpha family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: extracellular region

Molecular Function: hormone activity
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 Research Articles on GPHA2    1. Dual site-disrupted GPA2 and the GPB5 mutant were not expressed in either the conditioned medium or cell lysate.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with GPHA2 recombinant proteinOrgans/Tissues associated with GPHA2 recombinant protein
 Reference Product  PubMed Publications
 GPHB5 recombinant protein  >1 publications with GPHA2 and GPHB5
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1 publications with GPHA2 and Blood
 Brain Antibodies  >1 publications with GPHA2 and Brain
 Testis Antibodies  >1 publications with GPHA2 and Testis
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