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TLR4 recombinant protein :: Toll Like Receptor 4 (TLR4) Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS2009564 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
SDS-PAGE
Unit / Price
0.01 mg  /  $130 +1 FREE 8GB USB
0.05 mg  /  $225 +1 FREE 8GB USB
0.1 mg  /  $340 +1 FREE 8GB USB
0.2 mg  /  $415 +1 FREE 8GB USB
0.5 mg  /  $800 +1 FREE 8GB USB
1 mg  /  $1,180 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Toll Like Receptor 4 (TLR4), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Toll Like Receptor 4 (TLR4)

 Product Gene Name   

TLR4 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF-NPCIE VVPNITYQCM DQKLSKVPDD IPSSTKNIDL SFNPLKILKS YSFSNFSELQ WLDLSRCEIE TIEDKAWHGL HHLSNLILTG NPIQSFSPGS FSGLTSLENL VAVETKLASL ESFPIGQLIT LKKLNVAHNF IHSCKLPAYF SNLTNLVHVD LSYNYIQTIT VNDLQFLREN PQVNLSLDMS LNPIDFIQDQ AFQGIKLHEL TLRGNFNSSN IMKTCLQNLA GLHVHRLILG EFKDERNLE
 Chromosome Location    Chromosome: 4; NC_000070.6 (66827811..66842797). Location: 4 C1; 4 34.66 cM
 3D Structure    ModBase 3D Structure for Q9QUK6
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 Host    E Coli
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 Purity/Purification    > 95%
 Form/Format    Supplied as lyophilized form in 10mM PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and preservative.
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 Organism    Mus musculus (Mouse)
 Expression System    Prokaryotic expression
 Residues    Asn26~Glu269 (Accession # Q9QUK6) with two N-terminal Tags, His-tag and S-tag
 Subcellular Location    Membrane; Single-pass type I membrane protein.
 Predicted isoelectric point    5.7
 Predicted Molecular Mass    33.3kDa
 Endotoxin Level    <1.0EU per 1ug (determined by the LAL method)
 Reconstitution    Reconstitute in ddH2O.
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 Preparation and Storage    Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
 Other Notes    Small volumes of TLR4 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for TLR4 recombinant protein   

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

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 SDS-PAGE of TLR4 recombinant protein    TLR4 recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for TLR4. It may not necessarily be applicable to this product.
 NCBI GI #    10946594
 NCBI GeneID    21898
 NCBI Accession #    NP_067272.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_021297.2 [Other Products]
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 UniProt Primary Accession #    Q9QUK6 [Other Products]
 UniProt Secondary Accession #    Q9D691; Q9QZF5; Q9Z203 [Other Products]
 UniProt Related Accession #    Q9QUK6 [Other Products]
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 NCBI Official Full Name    toll-like receptor 4
 NCBI Official Synonym Full Names    toll-like receptor 4
 NCBI Official Symbol    Tlr4 [Similar Products]
 NCBI Official Synonym Symbols   
Lps; Ly87; Ran/M1; Rasl2-8
[Similar Products]
 NCBI Protein Information    toll-like receptor 4; lipopolysaccharide response
 UniProt Protein Name    Toll-like receptor 4
 Protein Family    Toll-like receptor
 UniProt Gene Name    Tlr4 [Similar Products]
 UniProt Synonym Gene Names    Lps [Similar Products]
 UniProt Entry Name    TLR4_MOUSE
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 NCBI Summary for TLR4    This gene belongs to the evolutionarily-conserved Toll-like receptor family, whose members are type-1 transmembrane proteins that are involved in innate immunity. Toll-like receptors are characterized by an extracellular leucine-rich repeat domain that functions in ligand recognition and an intracellular toll/interleukin-1 receptor-like domain that is crucial for signal transduction. The receptor encoded by this gene mediates the innate immune response to bacterial lipopolysaccharide, a major component of the outer membrane of Gram-negative bacteria, through synthesis of pro-inflammatory cytokines and chemokines. In addition, this protein can recognize other pathogens from Gram-negative and Gram-positive bacteria as well as viral components. Mice deficient in this gene display a number of immune response-related phenotypes including hyporesponsiveness to bacterial lipopolysaccharide and increased levels of respiratory syncytial virus compared to controls. [provided by RefSeq, Sep 2015]
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 UniProt Comments for TLR4    TLR4: Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS- independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific. Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR4. Binding to bacterial LPS leads to homodimerization. Interacts with LY96 via the extracellular domain. Interacts with MYD88 and TIRAP via their respective TIR domains. Interacts with NOX4. Interacts with CNPY3. Interacts with HSP90B1. The interaction with both CNPY3 and HSP90B1 is required for proper folding in the endoplasmic reticulum. Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells. Belongs to the Toll-like receptor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Cellular Component: cell surface; membrane; perinuclear region of cytoplasm; integral to plasma membrane; cytoplasm; integral to membrane; plasma membrane; lipopolysaccharide receptor complex; lipid raft; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; lipopolysaccharide binding; lipopolysaccharide receptor activity; phosphoinositide 3-kinase binding; receptor activity

Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of apoptosis; activation of MAPK activity; leukotriene metabolic process; response to lipopolysaccharide; positive regulation of NF-kappaB import into nucleus; positive regulation of interleukin-10 production; activation of NF-kappaB transcription factor; positive regulation of B cell proliferation; positive regulation of interferon-beta production; negative regulation of interleukin-6 production; toll-like receptor 4 signaling pathway; positive regulation of interferon-alpha production; positive regulation of DNA binding; positive regulation of I-kappaB kinase/NF-kappaB cascade; production of nitric oxide during acute inflammatory response; positive regulation of interleukin-8 biosynthetic process; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of chemokine production; macrophage activation; negative regulation of interleukin-23 production; positive regulation of peptidyl-tyrosine phosphorylation; response to ethanol; response to bacterium; detection of lipopolysaccharide; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; response to oxidative stress; I-kappaB phosphorylation; activation of innate immune response; positive regulation of nitric-oxide synthase biosynthetic process; regulation of cytokine secretion; positive regulation of interleukin-12 production; positive regulation of interleukin-6 biosynthetic process; microglial cell activation; positive regulation of JNK cascade; signal transduction; defense response to Gram-negative bacterium; mast cell activation; positive regulation of interleukin-1 biosynthetic process; positive regulation of stress-activated MAPK cascade; positive regulation of interleukin-8 production; positive regulation of lymphocyte proliferation; positive regulation of interleukin-13 biosynthetic process; positive regulation of MHC class II biosynthetic process; negative regulation of interferon-gamma production; lipopolysaccharide-mediated signaling pathway; interferon-gamma production; inflammatory response; innate immune response-activating signal transduction; B cell proliferation during immune response; positive regulation of tumor necrosis factor biosynthetic process; immune system process; activation of NF-kappaB-inducing kinase; negative regulation of tumor necrosis factor production; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interferon-gamma production; negative regulation of interleukin-17 production; positive regulation of interleukin-12 biosynthetic process; positive regulation of interferon-beta biosynthetic process; regulation of inflammatory response; toll-like receptor signaling pathway; innate immune response; immune response; regulation of sensory perception of pain; positive regulation of interleukin-1 production; positive regulation of inflammatory response
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 Research Articles on TLR4    1. These results suggest a pivotal role for TLR4 in mediating hind limb IR injury and suggest that NETs may contribute to muscle fiber injury.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TLR4 recombinant proteinPathways associated with TLR4 recombinant protein
 Reference Product  PubMed Publications
 Myd88 recombinant protein  >1521 publications with TLR4 and Myd88
 Ly96 recombinant protein  >289 publications with TLR4 and Ly96
 Traf6 recombinant protein  >172 publications with TLR4 and Traf6
 Ticam1 recombinant protein  >87 publications with TLR4 and Ticam1
 Traf3 recombinant protein  >14 publications with TLR4 and Traf3
 Ikbkg recombinant protein  >5 publications with TLR4 and Ikbkg
 Map3k7 recombinant protein  >4 publications with TLR4 and Map3k7
 Birc2 recombinant protein  >1 publications with TLR4 and Birc2
 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway antibodies  Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 HIF-1 Signaling Pathway antibodies  HIF-1 Signaling Pathway Diagram
 Hepatitis B Pathway antibodies  Hepatitis B Pathway Diagram
 IKK Complex Recruitment Mediated By RIP1 Pathway antibodies  IKK Complex Recruitment Mediated By RIP1 Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
Diseases associated with TLR4 recombinant proteinOrgans/Tissues associated with TLR4 recombinant protein
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >3312 publications with TLR4 and Inflammation
 Necrosis Antibodies  >2264 publications with TLR4 and Necrosis
 Sepsis Antibodies  >712 publications with TLR4 and Sepsis
 Neoplasms Antibodies  >521 publications with TLR4 and Neoplasms
 Liver Diseases Antibodies  >384 publications with TLR4 and Liver Diseases
 Atherosclerosis Antibodies  >370 publications with TLR4 and Atherosclerosis
 Heart Diseases Antibodies  >229 publications with TLR4 and Heart Diseases
 Fibrosis Antibodies  >195 publications with TLR4 and Fibrosis
 Carcinoma Antibodies  >183 publications with TLR4 and Carcinoma
 Kidney Diseases Antibodies  >175 publications with TLR4 and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >846 publications with TLR4 and Lung
 Liver Antibodies  >781 publications with TLR4 and Liver
 Bone Antibodies  >768 publications with TLR4 and Bone
 Bone Marrow Antibodies  >656 publications with TLR4 and Bone Marrow
 Brain Antibodies  >485 publications with TLR4 and Brain
 Spleen Antibodies  >379 publications with TLR4 and Spleen
 Kidney Antibodies  >376 publications with TLR4 and Kidney
 Muscle Antibodies  >362 publications with TLR4 and Muscle
 Heart Antibodies  >330 publications with TLR4 and Heart
 Skin Antibodies  >223 publications with TLR4 and Skin
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