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SUPT4H1 recombinant protein :: Transcription elongation factor SPT4 (SUPT4H1) Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS956520
SDS-PAGE
Unit / Price
0.01 mg (E-Coli)  /  $145 +1 FREE 8GB USB
0.05 mg (E-Coli)  /  $205 +1 FREE 8GB USB
0.1 mg (E-Coli)  /  $325 +1 FREE 8GB USB
0.2 mg (E-Coli)  /  $520 +1 FREE 8GB USB
0.05 mg (Yeast)  /  $675 +1 FREE 8GB USB
0.05 mg (Baculovirus)  /  $835 +1 FREE 8GB USB
0.5 mg (E-Coli)  /  $850 +1 FREE 8GB USB
0.2 mg (Yeast)  /  $915 +1 FREE 8GB USB
0.5 mg (Yeast)  /  $1,025 +1 FREE 8GB USB
0.1 mg (Baculovirus)  /  $1,050 +1 FREE 8GB USB
0.05 mg (Mammalian-Cell)  /  $1,070 +1 FREE 8GB USB
1 mg (E-Coli)  /  $1,340 +1 FREE 8GB USB
0.5 mg (Baculovirus)  /  $1,520 +1 FREE 8GB USB
1 mg (Yeast)  /  $1,560 +1 FREE 8GB USB
0.1 mg (Mammalian-Cell)  /  $1,635 +2 FREE 8GB USB
1 mg (Baculovirus)  /  $2,035 +2 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Transcription elongation factor SPT4 (SUPT4H1), Recombinant Protein

 Also Known As   

Recombinant Human Transcription elongation factor SPT4 (SUPT4H1)

 Product Synonym Names    Transcription elongation factor SPT4; hSPT4; DRB sensitivity-inducing factor 14 kDa subunit; DSIF p14; DRB sensitivity-inducing factor small subunit; DSIF small subunit
 Product Gene Name   

SUPT4H1 recombinant protein

[Similar Products]
 Product Synonym Gene Name    SUPT4H1; SPT4H; SUPT4H [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence Positions    1-117aa; Full Length
 Sequence    ALETVPKDLR HLRACLLCSL VKTIDQFEYD GCDNCDAYLQ MKGNREMVYD CTSSSFDGII AMMSPEDSWV SKWQRVSNFK PGVYAVSVTG RLPQGIVREL KSRGVAYKSR DTAIKT
 OMIM    603555
 3D Structure    ModBase 3D Structure for P63272
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 Host    E Coli or Yeast or Baculovirus or Mammalian Cell
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 Purity/Purification    >90%
 Form/Format    Liquid containing glycerol
 Tag Information    This protein contains an N-terminal tag and may also contain a C-terminal Myc-tag. N-terminal host tags may vary (His, His-SUMO, His-B2M, GST). Tag types are determined by various factors including tag-protein stability and, therefore, are subject to change; please inquire for tag information.
 Sterility    Sterile filter available upon request.
 Endotoxin    Low endotoxin available upon request.
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 Species    Homo sapiens (Human)
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 Preparation and Storage    Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of SUPT4H1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for SUPT4H1 recombinant protein

   Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.
 Product Categories/Family for SUPT4H1 recombinant protein    Epigenetics and Nuclear Signaling
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 SDS-PAGE of SUPT4H1 recombinant protein    SUPT4H1 recombinant protein SDS-PAGE image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
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NCBI/Uniprot data below describe general gene information for SUPT4H1. It may not necessarily be applicable to this product.
 NCBI GI #    4507311
 NCBI GeneID    6827
 NCBI Accession #    NP_003159.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_003168.2 [Other Products]
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 UniProt Primary Accession #    P63272 [Other Products]
 UniProt Secondary Accession #    Q16550; Q62387; Q6ZP89; B2R4X8; D3DTZ4 [Other Products]
 UniProt Related Accession #    P63272 [Other Products]
 Molecular Weight    13,193 Da
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 NCBI Official Full Name    transcription elongation factor SPT4
 NCBI Official Synonym Full Names    suppressor of Ty 4 homolog 1 (S. cerevisiae)
 NCBI Official Symbol    SUPT4H1 [Similar Products]
 NCBI Official Synonym Symbols   
SPT4; SPT4H; SUPT4H
[Similar Products]
 NCBI Protein Information    transcription elongation factor SPT4; DSIF p14; small hSpt4 subunit; DRB sensitivity-inducing factor 14 kDa subunit
 UniProt Protein Name    Transcription elongation factor SPT4
 UniProt Synonym Protein Names   
DRB sensitivity-inducing factor 14 kDa subunit; DSIF p14; DRB sensitivity-inducing factor small subunit; DSIF small subunit
 UniProt Gene Name    SUPT4H1 [Similar Products]
 UniProt Synonym Gene Names    SPT4H; SUPT4H; hSPT4; DSIF p14; DSIF small subunit [Similar Products]
 UniProt Entry Name    SPT4H_HUMAN
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 NCBI Summary for SUPT4H1    This gene encodes the small subunit of DRB (5,6-dichloro-1-beta-d-ribofuranosylbenzimidazole) sensitivity-inducing factor (DSIF) complex, which regulates mRNA processing and transcription elongation by RNA polymerase II. The encoded protein is localized to the nucleus and interacts with the large subunit (SUPT5H) to form the DSIF complex. Related pseudogenes have been identified on chromosomes 2 and 12. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012]
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 UniProt Comments for SUPT4H1    SUPT4H1: Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV- 1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences. Belongs to the SPT4 family.

Chromosomal Location of Human Ortholog: 17q22

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; zinc ion binding; protein heterodimerization activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; chromatin remodeling; regulation of transcription from RNA polymerase II promoter; viral reproduction; regulation of transcription, DNA-dependent; positive regulation of viral transcription; positive regulation of RNA elongation; RNA elongation from RNA polymerase II promoter; gene expression; positive regulation of transcription from RNA polymerase II promoter; negative regulation of RNA elongation; negative regulation of transcription from RNA polymerase II promoter
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 Research Articles on SUPT4H1    1. crystal structure of hSpt4 in complex with the dimerization region of hSpt5
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with SUPT4H1 recombinant proteinPathways associated with SUPT4H1 recombinant protein
 Reference Product  PubMed Publications
 SUPT5H recombinant protein  >4 publications with SUPT4H1 and SUPT5H
 Products by Pathway  Pathway Diagram
 Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway antibodies  Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway antibodies  Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway Diagram
 Formation Of HIV-1 Elongation Complex In The Absence Of HIV-1 Tat Pathway antibodies  Formation Of HIV-1 Elongation Complex In The Absence Of HIV-1 Tat Pathway Diagram
 Formation Of RNA Pol II Elongation Complex Pathway antibodies  Formation Of RNA Pol II Elongation Complex Pathway Diagram
 Formation Of The Early Elongation Complex Pathway antibodies  Formation Of The Early Elongation Complex Pathway Diagram
 Formation Of The HIV-1 Early Elongation Complex Pathway antibodies  Formation Of The HIV-1 Early Elongation Complex Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 HIV Infection Pathway antibodies  HIV Infection Pathway Diagram
 HIV Life Cycle Pathway antibodies  HIV Life Cycle Pathway Diagram
Diseases associated with SUPT4H1 recombinant proteinOrgans/Tissues associated with SUPT4H1 recombinant protein
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >2 publications with SUPT4H1 and Neoplasms
 Necrosis Antibodies  >1 publications with SUPT4H1 and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >2 publications with SUPT4H1 and Embryonic Tissue
 Salivary Gland Antibodies  >1 publications with SUPT4H1 and Salivary Gland
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