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SPI1 recombinant protein :: Transcription factor PU.1 Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS1265403
SDS-PAGE
Unit / Price
0.05 mg (Yeast)  /  $810 +1 FREE 8GB USB
0.05 mg (Baculovirus)  /  $955 +1 FREE 8GB USB
0.2 mg (Yeast)  /  $1,085 +1 FREE 8GB USB
0.05 mg (Mammalian-Cell)  /  $1,195 +1 FREE 8GB USB
0.1 mg (Baculovirus)  /  $1,200 +1 FREE 8GB USB
0.5 mg (Yeast)  /  $1,230 +1 FREE 8GB USB
0.5 mg (Baculovirus)  /  $1,730 +2 FREE 8GB USB
0.1 mg (Mammalian-Cell)  /  $1,810 +2 FREE 8GB USB
1 mg (Yeast)  /  $1,865 +2 FREE 8GB USB
1 mg (Baculovirus)  /  $2,310 +2 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Transcription factor PU.1 (SPI1), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Human Transcription factor PU.1

 Product Synonym Names    31 kDa-transforming protein
 Product Gene Name   

SPI1 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 MBS1265403 COA    COA PDF
 Sequence Positions    1-270aa; Full Length
 Sequence    MLQACKMEGF PLVPPPSEDL VPYDTDLYQR QTHEYYPYLS SDGESHSDHY WDFHPHHVHS EFESFAENNF TELQSVQPPQ LQQLYRHMEL EQMHVLDTPM VPPHPSLGHQ VSYLPRMCLQ YPSLSPAQPS SDEEEGERQS PPLEVSDGEA DGLEPGPGLL PGETGSKKKI RLYQFLLDLL RSGDMKDSIW WVDKDKGTFQ FSSKHKEALA HRWGIQKGNR KKMTYQKMAR ALRNYGKTGE VKKVKKKLTY QFSGEVLGRG GLAERRHPPH
 OMIM    165170
 3D Structure    ModBase 3D Structure for P17947
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 Host    E Coli or Yeast or Baculovirus or Mammalian Cell
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 Purity/Purification    Greater than 90% as determined by SDS-PAGE. (lot specific)
 Form/Format    Liquid containing glycerol
 Tag Information    This protein contains an N-terminal tag and may also contain a C-terminal Myc-tag. N-terminal host tags may vary (His, His-SUMO, His-B2M, GST). Tag types are determined by various factors including tag-protein stability and, therefore, are subject to change; please inquire for tag information.
 Sterility    Sterile filter available upon request.
 Endotoxin    Low endotoxin available upon request.
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 Preparation and Storage    Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of SPI1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for SPI1 recombinant protein

   Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.
 Product Categories/Family for SPI1 recombinant protein    Transcription
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 SDS-PAGE of SPI1 recombinant protein    SPI1 recombinant protein SDS-PAGE image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
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NCBI/Uniprot data below describe general gene information for SPI1. It may not necessarily be applicable to this product.
 NCBI GI #    124028521
 NCBI GeneID    6688
 NCBI Accession #    NP_001074016.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001080547.1 [Other Products]
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 UniProt Primary Accession #    P17947 [Other Products]
 UniProt Related Accession #    P17947 [Other Products]
 Molecular Weight    35.2kD
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 NCBI Official Full Name    transcription factor PU.1 isoform 1
 NCBI Official Synonym Full Names    Spi-1 proto-oncogene
 NCBI Official Symbol    SPI1 [Similar Products]
 NCBI Official Synonym Symbols   
OF; PU.1; SFPI1; SPI-1; SPI-A
[Similar Products]
 NCBI Protein Information    transcription factor PU.1
 UniProt Protein Name    Transcription factor PU.1
 UniProt Synonym Protein Names   
31 kDa-transforming protein
 Protein Family    Transcription factor
 UniProt Gene Name    SPI1 [Similar Products]
 UniProt Entry Name    SPI1_HUMAN
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 NCBI Summary for SPI1    This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for SPI1    PU.1: Binds to the PU-box, a purine-rich DNA sequence (5'- GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B- cells. Also binds RNA and may modulate pre-mRNA splicing. Binds DNA as a monomer. Interacts with CEBPD and NONO. Interacts with RUNX1 and SPIB. Interacts with GFI1; the interaction represses SPI1 transcriptional activity. Highly expressed in both FV-P and FV-A-induced erythro- leukemia cell lines that have undergone rearrangements of the Spi- 1 gene due to the insertion of SFFV. Belongs to the ETS family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: nuclear chromatin

Molecular Function: NFAT protein binding; protein binding; RNA binding; RNA polymerase II transcription factor activity, enhancer binding; transcription factor activity

Biological Process: anatomical structure regression; cell differentiation; erythrocyte differentiation; granulocyte differentiation; lymphocyte differentiation; lymphoid progenitor cell differentiation; macrophage differentiation; myeloid dendritic cell differentiation; negative regulation of gene expression, epigenetic; negative regulation of MHC class II biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of erythrocyte differentiation; somatic stem cell maintenance; transcription from RNA polymerase II promoter
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Product References and Citations for SPI1 recombinant protein

   The human homologue of the putative proto-oncogene Spi-1 characterization and expression in tumors.Ray D., Culine S., Tavitian A., Moreau-Gachelin F.Oncogene 5:663-668(1990) ErratumRay D., Culine S., Tavitian A., Moreau-Gachelin F.Oncogene 5:1611-1612(1990) Full-length cDNA libraries and normalization.Li W.B., Gruber C., Jessee J., Polayes D. Human chromosome 11 DNA sequence and analysis including novel gene identification.Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G., Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.Nature 440:497-500(2006) SPI-B activates transcription via a unique proline, serine, and threonine domain and exhibits DNA binding affinity differences from PU.1.Rao S., Matsumura A., Yoon J., Simon M.C.J. Biol. Chem. 274:11115-11124(1999) Functional and physical interactions between AML1 proteins and an ETS protein, MEF implications for the pathogenesis of t(8;21) -positive leukemias.Mao S., Frank R.C., Zhang J., Miyazaki Y., Nimer S.D.Mol. Cell. Biol. 19:3635-3644(1999) The transcriptional repressor GFI-1 antagonizes PU.1 activity through protein-protein interaction.Dahl R., Iyer S.R., Owens K.S., Cuylear D.D., Simon M.C.J. Biol. Chem. 282:6473-6483(2007) BCL6 positively regulates AID and germinal center gene expression via repression of miR-155.Basso K., Schneider C., Shen Q., Holmes A.B., Setty M., Leslie C., Dalla-Favera R.J. Exp. Med. 209:2455-2465(2012) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.J. Proteomics 96:253-262(2014)
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 Research Articles on SPI1    1. PU.1 suppressive target gene, metallothionein 1G, inhibits retinoic acid-induced NB4 cell differentiation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with SPI1 recombinant proteinPathways associated with SPI1 recombinant protein
 Reference Product  PubMed Publications
 RUNX1 recombinant protein  >12 publications with SPI1 and RUNX1
 GATA1 recombinant protein  >8 publications with SPI1 and GATA1
 GATA2 recombinant protein  >7 publications with SPI1 and GATA2
 IRF8 recombinant protein  >6 publications with SPI1 and IRF8
 IRF4 recombinant protein  >4 publications with SPI1 and IRF4
 FLT3 recombinant protein  >2 publications with SPI1 and FLT3
 CSF1R recombinant protein  >2 publications with SPI1 and CSF1R
 TBP recombinant protein  >2 publications with SPI1 and TBP
 Products by Pathway  Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Glucocorticoid Receptor Regulatory Network Pathway antibodies  Glucocorticoid Receptor Regulatory Network Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 IL-3 Signaling Pathway antibodies  IL-3 Signaling Pathway Diagram
 IL-4 Signaling Pathway antibodies  IL-4 Signaling Pathway Diagram
Diseases associated with SPI1 recombinant proteinOrgans/Tissues associated with SPI1 recombinant protein
 Disease Name  Pubmed Publications
 Leukemia, Myeloid Antibodies  >26 publications with SPI1 and Leukemia, Myeloid
 Inflammation Antibodies  >25 publications with SPI1 and Inflammation
 Leukemia, Myeloid, Acute Antibodies  >23 publications with SPI1 and Leukemia, Myeloid, Acute
 Disease Models, Animal Antibodies  >17 publications with SPI1 and Disease Models, Animal
 Liver Diseases Antibodies  >7 publications with SPI1 and Liver Diseases
 Cardiovascular Diseases Antibodies  >7 publications with SPI1 and Cardiovascular Diseases
 Neoplasms, Experimental Antibodies  >5 publications with SPI1 and Neoplasms, Experimental
 Necrosis Antibodies  >5 publications with SPI1 and Necrosis
 Lung Diseases Antibodies  >5 publications with SPI1 and Lung Diseases
 Atherosclerosis Antibodies  >4 publications with SPI1 and Atherosclerosis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >61 publications with SPI1 and Blood
 Intestine Antibodies  >46 publications with SPI1 and Intestine
 Spleen Antibodies  >25 publications with SPI1 and Spleen
 Liver Antibodies  >13 publications with SPI1 and Liver
 Heart Antibodies  >9 publications with SPI1 and Heart
 Lymph Node Antibodies  >6 publications with SPI1 and Lymph Node
 Lymph Antibodies  >5 publications with SPI1 and Lymph
 Lung Antibodies  >5 publications with SPI1 and Lung
 Kidney Antibodies  >4 publications with SPI1 and Kidney
 Brain Antibodies  >3 publications with SPI1 and Brain
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