Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Recombinant Protein rightarrow TNNI1  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • PDGFRbeta ELISA Kit
 • dentin sialophosphoprotein (DSPP) ELISA Kit
 • A Disintegrin and Metalloprotease 18 (ADAM18) ELISA Kit
 • AP-3 complex subunit beta-1 (AP3B1) Recombinant Protein
 • Factor VIII (F8) Antibody
 • Podoplanin (PDPN) ELISA Kit
 • lactoperoxidase (LPO) ELISA Kit
 • Cerebellin 1 (CBLN1) ELISA Kit
 • PDIA3 - ERp57 - GRP58 Peptide
 • Brain-derived neurotrophic factor (BDNF) Antibody
 • Actin Beta (ACTb) Recombinant Protein
 • Kinase Akt1-PKB alpha Active Enzyme (PKAkt1-PKBa) Recombinant Protein
 • ADRBK1 Antibody
 • Thioredoxin (Trx) Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

TNNI1 recombinant protein :: Troponin I Type 1, Slow Skeletal (TNNI1) Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS2012003 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
0.01 mg  /  $135 +1 FREE 8GB USB
0.05 mg  /  $245 +1 FREE 8GB USB
0.1 mg  /  $365 +1 FREE 8GB USB
0.2 mg  /  $450 +1 FREE 8GB USB
0.5 mg  /  $870 +1 FREE 8GB USB
1 mg  /  $1,280 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Troponin I Type 1, Slow Skeletal (TNNI1), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Troponin I Type 1, Slow Skeletal (TNNI1)

 Product Gene Name   

TNNI1 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 Sequence    The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF-MPEVER KPKI TASRKLLLKS LMLAKAKECW EQEHEEREAE KVRYLAERIP TLQTRGLSLS ALQDLCRELH AKVEVVDEER YDIEAKCLHN TREIKDLKLK VMDLRGKFKR PPLRRVRVSA DAMLRALLGS KHKVSMDLRA NLKSVKKEDT EKERPVEVGD WRKNVEAMSG MEGRKKMFDA AKSPTSQ
 Chromosome Location    Chromosome: 1; NC_000001.10 (201372895..201390874, complement). Location: 1q31.3
 OMIM    191042
 3D Structure    ModBase 3D Structure for P19237
Section Bar
 Host    E Coli
Section Bar
 Purity/Purification    > 95%
 Form/Format    Supplied as lyophilized form in PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and preservative.
Section Bar
 Organism    Homo sapiens (Human)
 Expression System    Prokaryotic expression
 Residues    Met1~Gln187 (Accession # P19237) with N-terminal His-Tag
 Predicted isoelectric point    9.5
 Endotoxin Level    <1.0EU per 1ug (determined by the LAL method)
 Reconstitution    Reconstitute in sterile PBS, pH7.2-pH7.4.
Section Bar
 Preparation and Storage    Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Other Notes    Small volumes of TNNI1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 

Related Product Information for TNNI1 recombinant protein

   About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
 Applications Tested/Suitable for TNNI1 recombinant protein   

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

Section Bar
 SDS-PAGE of TNNI1 recombinant protein    TNNI1 recombinant protein SDS-PAGE image
Section Bar
 SDS-PAGE of TNNI1 recombinant protein    [ DESCRIPTION]
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10 kDa, 14 kDa, 18 kDa, 22 kDa, 26 kDa, 33 kDa, 44 kDa and 70 kDa.
Double intensity bands: The 26 kDa, 18 kDa, 10 kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
[ STORAGE BUFFER]
62.5mM Tris-H3P04 (pH 7.5 at 25C), 1 mM EDTA, 2% (w/v) SDS, 100mM DTT, 1 mM NaN3, 0.01 % (w/v) bromo-phenol blue and 33% (v/v) glycerol.
[APPLICATION]
Accurate protein sizing on SDS-polyacrylamide gels and Western blots.
[USAGE]
1. Allow the Marker to reach room temperature and thoroughly mix before use. This will ensure that any solids that may have precipitated at -20C have returned to solution. Do not boil!
2. Load the following volumes of the Marker on a SDS-polyacrylamide gel.
[ LOADING VOLUMES]
3~5uL per well for mini gel; 7uL per well for large gel.
Use the same volumes for Western blotting applications. The loading volumes listed above are recommended for gels with a thickness of 0.75~1.0mm. The loading volume should be double for 1.5mm thick gels.
[ QUALITY CONTROL]
The protein marker, with molecular weight shifts of < or = 5% and minimal band broadening, are confirmed by migration in SDS-PAGE system. Electrophoresis of the Marker on a 13~18% Tris-glycine SDS-polyacrylamide gel resolves 8 individual bands.
[IMPORTANT NOTES]
1. The protein marker is conveniently packaged and ready to use. There is no need to heat, dilute or add reducing agents. Do not boil, which may cause band degradation.
2. The molecular weights of the proteins have a lot-to-Iot variation of ~5%.
3. These protein markers are for SDS-PAGE and should not to be used for native electrophoresis or a native gel. These markers are denatured and have SDS in the storage buffer.
4. Don't load too much protein. See recommended load volumes in the manual.
5. Check % gel that is being used if the lands miss. Depending on gel type and/or percentage, you may not see all of the bands. For example, one would not see the smallest bands of the standard on a very low % gel. A high % gel may not resolve the higher MW bands. Homogeneous low percentage gels are recommended for analysis of large proteins and high percentage gels for analysis of small proteins. In high percentage gels (13~18%) large proteins (22~70kDa) can separate, while in low percentage gels (4~8%) small proteins (14 and 10kDa) will migrate with the tracking dye. Longer transfer times or higher transfer voltages may be required for Western blotting of large (>100kDa) proteins.
6. If additional bands are observed in the gel image of the protein ladder, this might be caused by DTT oxidation in the storage buffer.
7. If proteins show a poor transfer, increase voltage, current or length of time for transfer, and pay attention to the SDS from the transfer buffer. SDS will cause the proteins to bind less efficiently to membranes because it disrupts the hydrophobic interaction between the membrane and the protein. If SDS is present in transfer buffer, make sure that there is no more than 0.02% in buffer.
8. Alcohol enhances hydrophobic binding to membrane. Not enough alcohol may prevent binding.
[ SHELF LIFE]
Storage: -20 degree C, 1 year at -20 degree C. If the product has been stored as directed for one year, its shelf life maybe extended by adding dithiothreitol (Dn) to approximately 50mM.
TNNI1 recombinant protein SDS-PAGE image
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for TNNI1. It may not necessarily be applicable to this product.
 NCBI GI #    56682969
 NCBI GeneID    7135
 NCBI Accession #    NP_003272.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_003281.3 [Other Products]
Section Bar
 UniProt Primary Accession #    P19237 [Other Products]
 UniProt Secondary Accession #    Q659A5; Q6FGS7; Q6FGW1; Q6ICU2; Q86T57; Q96DT9; A6NEH3; A8MSJ0 [Other Products]
 UniProt Related Accession #    P19237 [Other Products]
 Molecular Weight    23.2kDa
Table BarTOPTable Bar
 NCBI Official Full Name    troponin I, slow skeletal muscle
 NCBI Official Synonym Full Names    troponin I type 1 (skeletal, slow)
 NCBI Official Symbol    TNNI1 [Similar Products]
 NCBI Official Synonym Symbols   
TNN1; SSTNI
[Similar Products]
 NCBI Protein Information    troponin I, slow skeletal muscle; troponin I, slow-twitch isoform
 UniProt Protein Name    Troponin I, slow skeletal muscle
 UniProt Synonym Protein Names   
Troponin I, slow-twitch isoform
 Protein Family    Troponin
 UniProt Gene Name    TNNI1 [Similar Products]
 UniProt Entry Name    TNNI1_HUMAN
Section Bar
 NCBI Summary for TNNI1    Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for TNNI1    TNNI1: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Belongs to the troponin I family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 1q31.3

Cellular Component: troponin complex; cytosol

Molecular Function: metal ion binding; actin binding

Biological Process: regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; muscle filament sliding
Table BarTOPTable Bar
 Research Articles on TNNI1    1. sTnI as a sensitive marker of skeletal muscle injury.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Pathways associated with TNNI1 recombinant proteinDiseases associated with TNNI1 recombinant protein
 Products by Pathway  Pathway Diagram
 Muscle Contraction Pathway antibodies  Muscle Contraction Pathway Diagram
 Striated Muscle Contraction Pathway antibodies  Striated Muscle Contraction Pathway Diagram
 Striated Muscle Contraction Pathway antibodies  Striated Muscle Contraction Pathway Diagram
 Disease Name  Pubmed Publications
 Heart Diseases Antibodies  >1 publications with TNNI1 and Heart Diseases
 Cardiovascular Diseases Antibodies  >1 publications with TNNI1 and Cardiovascular Diseases
 Necrosis Antibodies  >1 publications with TNNI1 and Necrosis
 Hypertrophy Antibodies  >1 publications with TNNI1 and Hypertrophy
Organs/Tissues associated with TNNI1 recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >15 publications with TNNI1 and Muscle
 Heart Antibodies  >7 publications with TNNI1 and Heart
 Brain Antibodies  >1 publications with TNNI1 and Brain
 Spinal Cord Antibodies  >1 publications with TNNI1 and Spinal Cord
 Embryonic Tissue Antibodies  >1 publications with TNNI1 and Embryonic Tissue
 Lung Antibodies  >1 publications with TNNI1 and Lung
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions