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TPSb2 recombinant protein :: Tryptase Beta 2 Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS2031483 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
0.01 mg  /  $145 +1 FREE 8GB USB
0.05 mg  /  $285 +1 FREE 8GB USB
0.1 mg  /  $445 +1 FREE 8GB USB
0.2 mg  /  $545 +1 FREE 8GB USB
0.5 mg  /  $1,055 +1 FREE 8GB USB
1 mg  /  $1,575 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Tryptase Beta 2 (TPSb2), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Tryptase Beta 2 (TPSb2)

 Product Gene Name   

TPSb2 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    191081
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 Host    E. coli
 Species Reactivity    Rat
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 Purity/Purification    >98%
 Form/Format    20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
 Concentration    200ug/mL (lot specific)
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 Source    Prokaryotic expression
 Residues    Ile30~Ser274
 Tags    N-terminal His-Tag
 Subcellular Location    Secreted
 Traits    Freeze-dried powder
    
 Predicted isoelectric point    5.9
 Predicted Molecular Mass    31.2kDa
 Accurate Molecular Mass    35kDa as determined by SDS-PAGE reducing conditions
 Phenomenon explanation    The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
 Usage    Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
    
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 Preparation and Storage    Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
 Other Notes    Small volumes of TPSb2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for TPSb2 recombinant protein   

SDS-PAGE, WB, ELISA, IP, CoIP, Purification, Amine Reactive Labeling.

(May be suitable for use in other assays to be determined by the end user.)

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 Sequence of TPSb2 recombinant protein    TPSb2 recombinant protein Sequence image
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 Gene Sequence Extract of TPSb2 recombinant protein    TPSb2 recombinant protein Gene Sequence Extract image
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 SDS-PAGE of TPSb2 recombinant protein    TPSb2 recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for TPSb2. It may not necessarily be applicable to this product.
 NCBI GI #    68508970
 NCBI GeneID    64499
 NCBI Accession #    NP_077078.5 [Other Products]
 NCBI GenBank Nucleotide #    NM_024164.5 [Other Products]
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 UniProt Secondary Accession #    O95827; Q15664; Q9UQI6; Q9UQI7; D2E6S0; D2E6S2 [Other Products]
 UniProt Related Accession #    P20231 [Other Products]
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 NCBI Official Full Name    tryptase beta-2 preproprotein
 NCBI Official Synonym Full Names    tryptase beta 2 (gene/pseudogene)
 NCBI Official Symbol    TPSB2 [Similar Products]
 NCBI Official Synonym Symbols   
TPS2; tryptaseB; tryptaseC
[Similar Products]
 NCBI Protein Information    tryptase beta-2
 UniProt Protein Name    Tryptase beta-2
 UniProt Synonym Protein Names   
Tryptase II
 Protein Family    Tryptase
 UniProt Gene Name    TPSB2 [Similar Products]
 UniProt Synonym Gene Names    TPS2; Tryptase-2 [Similar Products]
 UniProt Entry Name    TRYB2_HUMAN
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 NCBI Summary for TPSb2    Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, beta II and beta III. Beta tryptases appear to be the main isoenzymes expressed in mast cells, whereas in basophils, alpha-tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq, Jul 2008]
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 UniProt Comments for TPSb2    TPSB2: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Has an immunoprotective role during bacterial infection. Required to efficiently combat K.pneumoniae infection. Belongs to the peptidase S1 family. Tryptase subfamily.

Protein type: Secreted, signal peptide; Secreted; EC 3.4.21.59; Protease

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: extracellular region

Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: proteolysis
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 Research Articles on TPSb2    1. These observations indicate that betaII-tryptase activity is post-translationally regulated by an allosteric disulfide bond.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TPSb2 recombinant proteinDiseases associated with TPSb2 recombinant protein
 Reference Product  PubMed Publications
 CPA3 recombinant protein  >3 publications with TPSb2 and CPA3
 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >5 publications with TPSb2 and Immune System Diseases
 Inflammation Antibodies  >5 publications with TPSb2 and Inflammation
 Lung Diseases Antibodies  >2 publications with TPSb2 and Lung Diseases
 Edema Antibodies  >1 publications with TPSb2 and Edema
Organs/Tissues associated with TPSb2 recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >2 publications with TPSb2 and Skin
 Bone Antibodies  >1 publications with TPSb2 and Bone
 Bone Marrow Antibodies  >1 publications with TPSb2 and Bone Marrow
 Lung Antibodies  >1 publications with TPSb2 and Lung
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