Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Recombinant Protein rightarrow TNF a  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Fibroblast Growth Factor 23 (FGF-23) ELISA Kit
 • TUBalpha3C ELISA Kit
 • AS160 (Phospho-Thr642) Antibody
 • HM13 Antibody
 • FPR1 cDNA Clone
 • ARNT Antibody
 • TSH Antibody
 • AST ELISA Kit
 • alpha-L-iduronidase (IDUA) ELISA Kit
 • Serum Secondary Antibody
 • Epinephrine-Adrenaline ELISA Kit
 • Carnosine Synthase 1 (CARNS1) Antibody
 • Steroid hormone receptor ERR1 (ESRRA) Recombinant Protein
 • PPEF1 Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

TNF a recombinant protein :: Tumor Necrosis Factor-alpha Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS142100 TNF a recombinant protein
Unit / Price
0.01 mg  /  $140 +1 FREE 8GB USB
0.05 mg  /  $205 +1 FREE 8GB USB
1 mg  /  $1,365 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Tumor Necrosis Factor-alpha (TNF a), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Human Tumor Necrosis Factor-alpha

 Product Synonym Names    TNF a Human; Tumor Necrosis Factor-Alpha Human Recombinant; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Cachectin; DIF; TNFA; TNFSF2
 Product Gene Name   

TNF a recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 Sequence    MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA E AKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL
 OMIM    191160
 3D Structure    ModBase 3D Structure for P01375
Section Bar
 Host    E Coli
Section Bar
 Purity/Purification    Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
 Form/Format    1mg of TNF-alpha Human contain 20mM PB, pH-7.2, and 100mM NaCl.
Sterile Filtered White lyophilized (freeze-dried) powder.
Section Bar
 Solubility    It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Section Bar
 Preparation and Storage    Lyophilized Tumor Necrosis Factor-a although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution TNF-a should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
 Other Notes    Small volumes of TNF a recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 

Related Product Information for TNF a recombinant protein

   Description: Tumor Necrosis Factor-a Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 158 amino acids (157 a.a. of the mature human TNF-alpha and an N-terminal methionine) and having a molecular mass of 17.5kDa. The TNF-alpha is purified by standard chromatographic techniques.

Introduction: Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages. TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF's primary role is in the regulation of immune cells.Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.
 Product Categories/Family for TNF a recombinant protein    CYTOKINES AND GROWTH FACTORS; Cytokines; Tumor Necrosis Factor
NCBI/Uniprot data below describe general gene information for TNF a. It may not necessarily be applicable to this product.
 NCBI GI #    25952111
 NCBI GeneID    7124
 NCBI Accession #    NP_000585.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000594.3 [Other Products]
Section Bar
 UniProt Primary Accession #    P01375 [Other Products]
 UniProt Secondary Accession #    O43647; Q9P1Q2; Q9UIV3 [Other Products]
 UniProt Related Accession #    P01375 [Other Products]
 Molecular Weight    25,644 Da
Table BarTOPTable Bar
 NCBI Official Full Name    tumor necrosis factor
 NCBI Official Synonym Full Names    tumor necrosis factor
 NCBI Official Symbol    TNF [Similar Products]
 NCBI Official Synonym Symbols   
DIF; TNFA; TNFSF2; TNF-alpha
[Similar Products]
 NCBI Protein Information    tumor necrosis factor; APC1 protein; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; cachectin; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha
 UniProt Protein Name    Tumor necrosis factor
 UniProt Synonym Protein Names   
Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
 UniProt Gene Name    TNF [Similar Products]
 UniProt Synonym Gene Names    TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2 [Similar Products]
 UniProt Entry Name    TNFA_HUMAN
Section Bar
 NCBI Summary for TNF a    This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for TNF a    TNF-a: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Homotrimer. Interacts with SPPL2B. Belongs to the tumor necrosis factor family.

Protein type: Motility/polarity/chemotaxis; Cytokine; Apoptosis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: extracellular space; recycling endosome; cell surface; integral to plasma membrane; extracellular region; plasma membrane; phagocytic cup; external side of plasma membrane; lipid raft

Molecular Function: identical protein binding; protein binding; protease binding; cytokine activity; tumor necrosis factor receptor binding

Biological Process: positive regulation of JNK activity; extracellular matrix organization and biogenesis; positive regulation of nitric oxide biosynthetic process; positive regulation of NFAT protein import into nucleus; positive regulation of osteoclast differentiation; activation of MAPK activity; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; response to glucocorticoid stimulus; positive regulation of caspase activity; positive regulation of NF-kappaB import into nucleus; osteoclast differentiation; positive regulation of translational initiation by iron; positive regulation of membrane protein ectodomain proteolysis; activation of NF-kappaB transcription factor; positive regulation of MAP kinase activity; tumor necrosis factor-mediated signaling pathway; positive regulation of phagocytosis; negative regulation of interleukin-6 production; JNK cascade; negative regulation of osteoblast differentiation; positive regulation of action potential; regulation of immunoglobulin secretion; embryonic gut development; negative regulation of protein complex disassembly; positive regulation of cytokine production; response to drug; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of heterotypic cell-cell adhesion; positive regulation of mitosis; response to virus; positive regulation of interleukin-8 biosynthetic process; positive regulation of interleukin-6 production; glucose metabolic process; negative regulation of fat cell differentiation; positive regulation of chemokine production; negative regulation of cytokine secretion during immune response; positive regulation of protein transport; cell activation; detection of mechanical stimulus involved in sensory perception of pain; defense response to Gram-positive bacterium; DNA damage response, signal transduction resulting in induction of apoptosis; induction of apoptosis via death domain receptors; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; negative regulation of L-glutamate transport; response to activity; negative regulation of transcription, DNA-dependent; skeletal muscle contraction; sequestering of triacylglycerol; positive regulation of smooth muscle cell proliferation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-18 production; chronic inflammatory response to antigenic stimulus; positive regulation of synaptic transmission; response to salt stress; positive regulation of hair follicle development; negative regulation of cell proliferation; response to radiation; negative regulation of lipid catabolic process; positive regulation of neuron apoptosis; protein kinase B signaling cascade; lipopolysaccharide-mediated signaling pathway; positive regulation of chronic inflammatory response to antigenic stimulus; regulation of I-kappaB kinase/NF-kappaB cascade; inflammatory response; caspase activation; positive regulation of humoral immune response mediated by circulating immunoglobulin; positive regulation of protein complex disassembly; transformed cell apoptosis; calcium-mediated signaling; MAPKKK cascade; positive regulation of peptidyl-serine phosphorylation; humoral immune response; positive regulation of protein kinase B signaling cascade; positive regulation of programmed cell death; negative regulation of glucose import; positive regulation of interferon-gamma production; positive regulation of protein complex assembly; positive regulation of chemokine biosynthetic process; negative regulation of viral genome replication; protein import into nucleus, translocation; positive regulation of protein kinase activity; activation of MAPKKK activity; positive regulation of fever; response to hypoxia; positive regulation of protein amino acid phosphorylation; receptor biosynthetic process; negative regulation of myoblast differentiation; leukocyte tethering or rolling; regulation of insulin secretion; positive regulation of cytokine secretion

Disease: Asthma, Susceptibility To; Migraine With Or Without Aura, Susceptibility To, 1; Malaria, Susceptibility To
Table BarTOPTable Bar
 Research Articles on TNF a    1. half- and full-marathon wheelchair races increased plasma IL-6, but not TNF-alpha and high-sensitivity C-reactive protein
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with TNF a recombinant proteinPathways associated with TNF a recombinant protein
 Reference Product  PubMed Publications
 ICAM1 recombinant protein  >3367 publications with TNF a and ICAM1
 CD40 recombinant protein  >1975 publications with TNF a and CD40
 IL6 recombinant protein  >1472 publications with TNF a and IL6
 TRAF2 recombinant protein  >1012 publications with TNF a and TRAF2
 TRADD recombinant protein  >523 publications with TNF a and TRADD
 TNFRSF1A recombinant protein  >428 publications with TNF a and TNFRSF1A
 IL1B recombinant protein  >286 publications with TNF a and IL1B
 RIPK1 recombinant protein  >189 publications with TNF a and RIPK1
 TNFRSF1B recombinant protein  >101 publications with TNF a and TNFRSF1B
 BIRC2 recombinant protein  >46 publications with TNF a and BIRC2
 Products by Pathway  Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 AhR Pathway antibodies  AhR Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
Diseases associated with TNF a recombinant proteinOrgans/Tissues associated with TNF a recombinant protein
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >90763 publications with TNF a and Necrosis
 Inflammation Antibodies  >32799 publications with TNF a and Inflammation
 Vascular Diseases Antibodies  >7582 publications with TNF a and Vascular Diseases
 Lung Diseases Antibodies  >6755 publications with TNF a and Lung Diseases
 Liver Diseases Antibodies  >5157 publications with TNF a and Liver Diseases
 Insulin Resistance Antibodies  >2522 publications with TNF a and Insulin Resistance
 Fibrosis Antibodies  >2491 publications with TNF a and Fibrosis
 Atherosclerosis Antibodies  >2416 publications with TNF a and Atherosclerosis
 Colitis Antibodies  >2412 publications with TNF a and Colitis
 Pain Antibodies  >2278 publications with TNF a and Pain
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >51567 publications with TNF a and Blood
 Lung Antibodies  >9706 publications with TNF a and Lung
 Brain Antibodies  >7040 publications with TNF a and Brain
 Heart Antibodies  >5075 publications with TNF a and Heart
 Intestine Antibodies  >3669 publications with TNF a and Intestine
 Connective Tissue Antibodies  >2284 publications with TNF a and Connective Tissue
 Lymph Node Antibodies  >1957 publications with TNF a and Lymph Node
 Placenta Antibodies  >840 publications with TNF a and Placenta
 Prostate Antibodies  >812 publications with TNF a and Prostate
 Ovary Antibodies  >551 publications with TNF a and Ovary
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions