Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Recombinant Protein rightarrow TNF  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Thromboxanes B2 (TXB2) ELISA Kit
 • CRP Recombinant Protein
 • UDP glucuronosyltransferase 2 family, polypeptide B7 (UGT2B7... ELISA Kit
 • Noggin ELISA Kit
 • Fcgamma ELISA Kit
 • Actin, alpha, Smooth Muscle (ACTA2) Antibody
 • fms-related tyrosine kinase 1 (vascular endothelial growth f... ELISA Kit
 • Inositol Triphosphate (IP3) ELISA Kit
 • Leukemia Inhibitory Factor (LIF) ELISA Kit
 • Gamma-Synuclein (SNCG) Antibody
 • TM ELISA Kit
 • Synaptopodin (Synpo) Antibody
 • A Disintegrin And Metalloproteinase With Thrombospondin 1 (A... Recombinant Protein
 • Annexin V (ANX-V) antibody (IgM) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

TNF recombinant protein :: Tumor Necrosis Factor-alpha Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS553156 TNF recombinant protein
Unit / Price
0.005 mg  /  $145 +1 FREE 8GB USB
0.02 mg  /  $220 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Tumor Necrosis Factor-alpha (TNF), Recombinant Protein

★Popular Item★
 Also Known As   

Mouse Tumor Necrosis Factor-alpha Recombinant

 Product Synonym Names    tnf-alpha; tumor necrosis factor; cachetin; tnfa
 Product Gene Name   

TNF recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 Sequence    MSTESMIRDV ELAEEALPQK MGGFQNSRRC LCLSLFSFLL VAGATTLFCL LNFGVIGPQR DEKFPNGLPL ISSMAQTLTL RSSSQNSSDK PVAHVVANHQ VEEQLEWLSQ RANALLANGM DLKDNQLVVP ADGLYLVYSQ VLFKGQGCPD YVLLTHTVSR FAISYQEKVN LLSAVKSPCP KDTPEGAELK PWYEPIYLGG VFQLEKGDQL SAEVNLPKYL DFAESGQVYF GVIAL
 3D Structure    ModBase 3D Structure for P06804
Section Bar
 Host    E Coli
Section Bar
 Purity/Purification    >97%, as determined by SDS-PAGE and HPLC
 Form/Format    Recombinant mouse TNF alpha was lyophilized from 0.2 um filtered PBS solution, pH 7.0.
Section Bar
 Domain    TNF
 Host Note    Optimized DNA sequence encoding Mouse Tumor Necrosis Factor-alpha extracellular domain was expressed in Escherichia Coli.
 Endotoxin    Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
 Reconstitution    A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
 Molecular Weight Note    Native Mouse Tumor Necrosis Factor-alpha is generated by the proteolytic removal of the signal peptide and propeptide, this molecule has a calculated mass of approximately 17kDa.

Recombinant Mouse TNF is a disulfide-linked monomer protein, consisting of 157 amino acids and migrates as an approximately 17 kDa protein under reducing conditions.
Section Bar
 Preparation and Storage    The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.
 Other Notes    Small volumes of TNF recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 

Product Description specifically for TNF recombinant protein

   TNF is secreted by macrophages, monocytes, neutrophils, T-cells, NK- cells following their stimulation by bacterial lipopolysaccharides. Cells expressing CD4 secrete TNF- alpha while CD8 (+) cells secrete little or no TNF- alpha. Stimulated peripheral neutrophilic granulocytes but also unstimulated cells and also a number of transformed cell lines, astrocytes, microglial cells, smooth muscle cells, and fibroblasts also secrete TNF. Human milk also contains this factor.
 Product Categories/Family for TNF recombinant protein    Cytokines
NCBI/Uniprot data below describe general gene information for TNF. It may not necessarily be applicable to this product.
 NCBI GI #    7305585
 NCBI GeneID    21926
 NCBI Accession #    NP_038721.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_013693.2 [Other Products]
Section Bar
 UniProt Primary Accession #    P06804 [Other Products]
 UniProt Secondary Accession #    O35853; Q62326; Q91VF3 [Other Products]
 UniProt Related Accession #    P06804; Q0X0E6; Q3U4I6; Q3U593; Q6TDG3 [Other Products]
 Molecular Weight    25,896 Da
Table BarTOPTable Bar
 NCBI Official Full Name    tumor necrosis factor
 UniProt Protein Name    Tumor necrosis factor
 UniProt Synonym Protein Names   
Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-aCleaved into the following 6 chains:Tumor necrosis factor, membrane form; Alternative name(s):; N-terminal fragment
 Protein Family    Tumor necrosis factor
 UniProt Gene Name    Tnf [Similar Products]
 UniProt Synonym Gene Names    Tnfa; Tnfsf2; TNF-a; NTF; ICD1; ICD2 [Similar Products]
 UniProt Entry Name    TNFA_MOUSE
Section Bar
 NCBI Summary for TNF    This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. Members of this family are classified based on primary sequence, function, and structure. This protein is synthesized as a type-II transmembrane protein and is reported to be cleaved into products that exert distinct biological functions. It plays an important role in the innate immune response as well as regulating homeostasis but is also implicated in diseases of chronic inflammation. In mouse deficiency of this gene is associated with defects in response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
Section Bar
 UniProt Comments for TNF    TNF-a: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Homotrimer. Interacts with SPPL2B. Belongs to the tumor necrosis factor family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Apoptosis; Cytokine

Cellular Component: extracellular space; recycling endosome; cell surface; membrane; integral to plasma membrane; extracellular region; plasma membrane; integral to membrane; intracellular; external side of plasma membrane; phagocytic cup; secretory granule; lipid raft

Molecular Function: identical protein binding; protein binding; protease binding; cytokine activity; tumor necrosis factor receptor binding

Biological Process: extracellular matrix organization and biogenesis; positive regulation of nitric oxide biosynthetic process; positive regulation of NFAT protein import into nucleus; activation of MAPK activity; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; positive regulation of caspase activity; positive regulation of translational initiation by iron; positive regulation of membrane protein ectodomain proteolysis; activation of NF-kappaB transcription factor; positive regulation of MAP kinase activity; cellular extravasation; tumor necrosis factor-mediated signaling pathway; positive regulation of phagocytosis; negative regulation of interleukin-6 production; JNK cascade; negative regulation of osteoblast differentiation; positive regulation of action potential; negative regulation of protein complex disassembly; positive regulation of cytokine production; positive regulation of heterotypic cell-cell adhesion; positive regulation of mitosis; response to virus; glucose metabolic process; positive regulation of interleukin-6 production; negative regulation of cytokine secretion during immune response; positive regulation of protein transport; DNA damage response, signal transduction resulting in induction of apoptosis; defense response to bacterium; positive regulation of transcription from RNA polymerase II promoter; sequestering of triacylglycerol; positive regulation of JNK cascade; negative regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-18 production; signal transduction; chronic inflammatory response to antigenic stimulus; positive regulation of hair follicle development; negative regulation of cell proliferation; positive regulation of neuron apoptosis; positive regulation of cell proliferation; protein kinase B signaling cascade; positive regulation of chronic inflammatory response to antigenic stimulus; inflammatory response; regulation of protein amino acid phosphorylation; transformed cell apoptosis; positive regulation of peptidyl-serine phosphorylation; humoral immune response; regulation of cell proliferation; cell proliferation; positive regulation of protein kinase B signaling cascade; positive regulation of programmed cell death; positive regulation of interferon-gamma production; positive regulation of protein complex assembly; negative regulation of viral genome replication; regulation of insulin secretion; positive regulation of JNK activity; positive regulation of osteoclast differentiation; multicellular organismal development; response to glucocorticoid stimulus; positive regulation of NF-kappaB import into nucleus; osteoclast differentiation; regulation of immunoglobulin secretion; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-8 biosynthetic process; positive regulation of chemokine production; cell activation; detection of mechanical stimulus involved in sensory perception of pain; organ morphogenesis; defense response to Gram-positive bacterium; induction of apoptosis via death domain receptors; positive regulation of transcription factor activity; negative regulation of L-glutamate transport; negative regulation of transcription, DNA-dependent; leukocyte migration; apoptosis; positive regulation of smooth muscle cell proliferation; defense response; positive regulation of synaptic transmission; regulation of osteoclast differentiation; regulation of protein secretion; negative regulation of lipid catabolic process; lipopolysaccharide-mediated signaling pathway; regulation of I-kappaB kinase/NF-kappaB cascade; caspase activation; positive regulation of humoral immune response mediated by circulating immunoglobulin; positive regulation of protein complex disassembly; MAPKKK cascade; calcium-mediated signaling; negative regulation of glucose import; positive regulation of chemokine biosynthetic process; protein import into nucleus, translocation; positive regulation of protein kinase activity; positive regulation of fever; activation of MAPKKK activity; immune response; positive regulation of protein amino acid phosphorylation; receptor biosynthetic process; negative regulation of myoblast differentiation; leukocyte tethering or rolling; positive regulation of inflammatory response; positive regulation of cytokine secretion
Table BarTOPTable Bar
 

Product References and Citations for TNF recombinant protein

   tak1-mediated serine/threonine phosphorylation of epidermal growth factor receptor via p38/extracellular signal-regulated kinase: nf-b-independent survival pathways in tumor necrosis factor alpha signaling .Mol. Cell. Biol., Oct 2009; 29: 5529 - 5539.
tumor necrosis factor--mediated regulation of the inositol 1,4,5-trisphosphate receptor promoter .J. Biol. Chem., Oct 2009; 284: 27557 - 27566.
tnf- inhibition attenuates adverse myocardial remodeling in a rat model of volume overload .Am J Physiol Heart Circ Physiol, Oct 2009; 297: H1462 - H1468.
tnf inhibits apoptotic cell clearance in the lung, exacerbating acute inflammation .Am J Physiol Lung Cell Mol Physiol, Oct 2009; 297: L586 - L595.
association of tnf- gene polymorphisms with systemic lupus erythematosus in taiwanese patients .Lupus, Oct 2009; 18: 974 - 979.
Section Bar
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with TNF recombinant proteinPathways associated with TNF recombinant protein
 Reference Product  PubMed Publications
 Tial1 recombinant protein  >6 publications with TNF and Tial1
 Nono recombinant protein  >5 publications with TNF and Nono
 Hnrnpd recombinant protein  >1 publications with TNF and Hnrnpd
 Ddx5 recombinant protein  >1 publications with TNF and Ddx5
 Products by Pathway  Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
Diseases associated with TNF recombinant proteinOrgans/Tissues associated with TNF recombinant protein
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >21448 publications with TNF and Inflammation
 Infection Antibodies  >12805 publications with TNF and Infection
 Neoplasms Antibodies  >11734 publications with TNF and Neoplasms
 Cardiovascular Diseases Antibodies  >6102 publications with TNF and Cardiovascular Diseases
 Lung Diseases Antibodies  >4957 publications with TNF and Lung Diseases
 Arthritis, Rheumatoid Antibodies  >4921 publications with TNF and Arthritis, Rheumatoid
 Sepsis Antibodies  >4127 publications with TNF and Sepsis
 Skin Diseases Antibodies  >4065 publications with TNF and Skin Diseases
 Liver Diseases Antibodies  >3609 publications with TNF and Liver Diseases
 Leukemia Antibodies  >2492 publications with TNF and Leukemia
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >3562 publications with TNF and Skin
 Spleen Antibodies  >3171 publications with TNF and Spleen
 Bone Marrow Antibodies  >3136 publications with TNF and Bone Marrow
 Joint Antibodies  >3112 publications with TNF and Joint
 Lymph Node Antibodies  >1543 publications with TNF and Lymph Node
 Embryonic Tissue Antibodies  >1431 publications with TNF and Embryonic Tissue
 Thymus Antibodies  >659 publications with TNF and Thymus
 Prostate Antibodies  >593 publications with TNF and Prostate
 Mammary Gland Antibodies  >141 publications with TNF and Mammary Gland
 Uncharacterized Tissue Antibodies  >13 publications with TNF and Uncharacterized Tissue
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions