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TP53 recombinant protein :: Tumor Protein p53 Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS2030765 (1 unit is in stock [0.1 mg] for immediate shipment)
Unit / Price
0.01 mg  /  $150 +1 FREE 8GB USB
0.05 mg  /  $290 +1 FREE 8GB USB
0.1 mg  /  $455 +1 FREE 8GB USB
0.2 mg  /  $560 +1 FREE 8GB USB
0.5 mg  /  $1,080 +1 FREE 8GB USB
1 mg  /  $1,610 +2 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Tumor Protein p53 (TP53), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Tumor Protein p53 (TP53)

 Product Gene Name   

TP53 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    133239
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 Host    E. coli
 Species Reactivity    Rat
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 Purity/Purification    >98%
 Form/Format    20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300
 Concentration    200ug/ml (lot specific)
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 Source    Prokaryotic expression.
 Residues    Pro90~Ala354
 Tags    N-terminal His-Tag
 Tissue Specificity    Heart, Brain, Kidney, Liver.
 Subcellular Location    Cytoplasm. Nucleus. Endoplasmic reticulum. Mitochondrion matrix.
 Traits    Freeze-dried powder
 Predicted isoelectric point    5.9
 Predicted Molecular Mass    33.7kDa
  Accurate Molecular Mass    36kDa as determined by SDS-PAGE reducing conditions.
 Usage    Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
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 Preparation and Storage    Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.
 Other Notes    Small volumes of TP53 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for TP53 recombinant protein   

SDS-PAGE, WB, ELISA, IP, CoIP, ReporterAssays, Purification, Amine Reactive Labeling.

(May be suitable for use in other assays to be determined by the end user.)

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 Sequence of TP53 recombinant protein    TP53 recombinant protein Sequence image
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 SDS-Page of TP53 recombinant protein    TP53 recombinant protein SDS-Page image
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NCBI/Uniprot data below describe general gene information for TP53. It may not necessarily be applicable to this product.
 NCBI GI #    13097807
 NCBI GeneID    7157
 NCBI Accession #    AAH03596.1 [Other Products]
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 UniProt Secondary Accession #    Q15086; Q15087; Q15088; Q16535; Q16807; Q16808; Q16809; Q16810; Q16811; Q16848; Q2XN98 [Other Products]
 UniProt Related Accession #    P04637 [Other Products]
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 NCBI Official Full Name    Tumor protein p53
 NCBI Official Synonym Full Names    tumor protein p53
 NCBI Official Symbol    TP53 [Similar Products]
 NCBI Official Synonym Symbols   
P53; BCC7; LFS1; TRP53
[Similar Products]
 NCBI Protein Information    cellular tumor antigen p53
 UniProt Protein Name    Cellular tumor antigen p53
 UniProt Synonym Protein Names   
Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53
 Protein Family    Tumor protein p53-inducible protein
 UniProt Gene Name    TP53 [Similar Products]
 UniProt Synonym Gene Names    P53 [Similar Products]
 UniProt Entry Name    P53_HUMAN
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 NCBI Summary for TP53    This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013]
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 UniProt Comments for TP53    p53: a transcription factor and major tumor suppressor that plays a major role in regulating cellular responses to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. More than 50 percent of human tumors contain a mutation or deletion of the TP53 gene. p53 is modified post-translationally at multiple sites. DNA damage induces phosphorylation of p53 at S15, S20 and S37, reducing its interaction with the oncoprotein MDM2. MDM2 inhibits p53 accumulation by targeting it for ubiquitination and proteasomal degradation. Phosphorylated by many kinases including Chk2 and Chk1 at S20, enhancing its tetramerization, stability and activity. The phosphorylation by CAK at S392 is increased in human tumors and has been reported to influence the growth suppressor function, DNA binding and transcriptional activation of p53. Phosphorylation of p53 at S46 regulates the ability of p53 to induce apoptosis. The acetylation of p53 appears to play a positive role in the accumulation of p53 during the stress response. Following DNA damage, p53 becomes acetylated at K382, enhancing its binding to DNA. Deacetylation of p53 can occur through interaction with SIRT1, a deacetylase that may be involved in cellular aging and the DNA damage response. p53 regulates the transcription of a set of genes encoding endosomal proteins that regulate endosomal functions. These include STEAP3 and CHMP4C, which enhance exosome production, and CAV1 and CHMP4C, which produce a more rapid endosomal clearance of the EGFR from the plasma membrane. DNA damage regulates a p53-mediated secretory pathway, increasing the secretion of some proteins such as Hsp90, SERPINE1, SERPINB5, NKEF-A, and CyPA, and inhibiting the secretion of others including CTSL and IGFBP-2. Two alternatively spliced human isoforms have been reported. Isoform 2 is expressed in quiescent lymphocytes. Seems to be non-functional. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Protein type: DNA-binding; Motility/polarity/chemotaxis; Transcription factor; Nuclear receptor co-regulator; Activator; Tumor suppressor

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: PML body; transcription factor TFIID complex; nuclear matrix; protein complex; mitochondrion; endoplasmic reticulum; replication fork; cytosol; nucleoplasm; nuclear body; mitochondrial matrix; cytoplasm; nuclear chromatin; nucleolus; nucleus; chromatin

Molecular Function: identical protein binding; protease binding; zinc ion binding; protein phosphatase 2A binding; p53 binding; protein N-terminus binding; receptor tyrosine kinase binding; protein phosphatase binding; transcription factor binding; protein kinase binding; histone acetyltransferase binding; protein binding; copper ion binding; enzyme binding; histone deacetylase regulator activity; DNA binding; protein heterodimerization activity; chaperone binding; ubiquitin protein ligase binding; damaged DNA binding; chromatin binding; transcription factor activity; ATP binding

Biological Process: viral reproduction; positive regulation of apoptosis; multicellular organismal development; positive regulation of transcription, DNA-dependent; T cell differentiation in the thymus; gastrulation; determination of adult life span; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; response to antibiotic; cellular response to glucose starvation; protein localization; negative regulation of neuroblast proliferation; base-excision repair; transforming growth factor beta receptor signaling pathway; cerebellum development; protein complex assembly; cell cycle arrest; ER overload response; response to X-ray; release of cytochrome c from mitochondria; somitogenesis; cell aging; chromatin assembly; circadian behavior; rRNA transcription; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of DNA replication; negative regulation of fibroblast proliferation; embryonic organ development; positive regulation of transcription from RNA polymerase II promoter; regulation of mitochondrial membrane permeability; negative regulation of transcription, DNA-dependent; regulation of tissue remodeling; negative regulation of apoptosis; G1 DNA damage checkpoint; transcription from RNA polymerase II promoter; DNA damage response, signal transduction by p53 class mediator; apoptosis; negative regulation of transcription from RNA polymerase II promoter; response to salt stress; entrainment of circadian clock by photoperiod; negative regulation of cell proliferation; positive regulation of protein oligomerization; positive regulation of histone deacetylation; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; regulation of transcription, DNA-dependent; T cell proliferation during immune response; double-strand break repair; positive regulation of neuron apoptosis; response to gamma radiation; cell differentiation; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; protein tetramerization; mitochondrial DNA repair; Notch signaling pathway; in utero embryonic development; multicellular organism growth; B cell lineage commitment; cell proliferation; neuron apoptosis; T cell lineage commitment; negative regulation of helicase activity; protein import into nucleus, translocation; nucleotide-excision repair; Ras protein signal transduction; DNA strand renaturation; negative regulation of cell growth; negative regulation of transforming growth factor beta receptor signaling pathway; blood coagulation; response to DNA damage stimulus

Disease: Papilloma Of Choroid Plexus; Pancreatic Cancer; Nasopharyngeal Carcinoma; Li-fraumeni Syndrome 1; Breast Cancer; Osteogenic Sarcoma; Colorectal Cancer; Glioma Susceptibility 1; Adrenocortical Carcinoma, Hereditary; Hepatocellular Carcinoma; Basal Cell Carcinoma, Susceptibility To, 7
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 Research Articles on TP53    1. Abrogation of the P53-mediated apoptosis response to DNA damage.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TP53 recombinant proteinPathways associated with TP53 recombinant protein
 Reference Product  PubMed Publications
 MDM2 recombinant protein  >871 publications with TP53 and MDM2
 CDKN2A recombinant protein  >739 publications with TP53 and CDKN2A
 CDKN1A recombinant protein  >667 publications with TP53 and CDKN1A
 BRCA1 recombinant protein  >438 publications with TP53 and BRCA1
 ATM recombinant protein  >424 publications with TP53 and ATM
 SIRT1 recombinant protein  >64 publications with TP53 and SIRT1
 EP300 recombinant protein  >32 publications with TP53 and EP300
 CREBBP recombinant protein  >19 publications with TP53 and CREBBP
 MAPK8 recombinant protein  >7 publications with TP53 and MAPK8
 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Activation Of BH3-only Proteins Pathway antibodies  Activation Of BH3-only Proteins Pathway Diagram
 Activation Of NOXA And Translocation To Mitochondria Pathway antibodies  Activation Of NOXA And Translocation To Mitochondria Pathway Diagram
 Activation Of PUMA And Translocation To Mitochondria Pathway antibodies  Activation Of PUMA And Translocation To Mitochondria Pathway Diagram
 Alzheimers Disease Pathway antibodies  Alzheimers Disease Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
Diseases associated with TP53 recombinant proteinOrgans/Tissues associated with TP53 recombinant protein
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >3101 publications with TP53 and Carcinoma
 Breast Neoplasms Antibodies  >1178 publications with TP53 and Breast Neoplasms
 Colorectal Neoplasms Antibodies  >781 publications with TP53 and Colorectal Neoplasms
 Carcinoma, Squamous Cell Antibodies  >733 publications with TP53 and Carcinoma, Squamous Cell
 Glioma Antibodies  >619 publications with TP53 and Glioma
 Neoplasm Metastasis Antibodies  >451 publications with TP53 and Neoplasm Metastasis
 Carcinoma, Small Cell Antibodies  >448 publications with TP53 and Carcinoma, Small Cell
 Cell Transformation, Neoplastic Antibodies  >432 publications with TP53 and Cell Transformation, Neoplastic
 Liver Neoplasms Antibodies  >389 publications with TP53 and Liver Neoplasms
 Sarcoma Antibodies  >381 publications with TP53 and Sarcoma
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1002 publications with TP53 and Blood
 Lung Antibodies  >872 publications with TP53 and Lung
 Brain Antibodies  >669 publications with TP53 and Brain
 Liver Antibodies  >493 publications with TP53 and Liver
 Bone Antibodies  >366 publications with TP53 and Bone
 Lymph Node Antibodies  >340 publications with TP53 and Lymph Node
 Skin Antibodies  >339 publications with TP53 and Skin
 Lymph Antibodies  >332 publications with TP53 and Lymph
 Bladder Antibodies  >308 publications with TP53 and Bladder
 Prostate Antibodies  >249 publications with TP53 and Prostate
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