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Dio3 recombinant protein :: Type III iodothyronine deiodinase (Dio3) Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS1116133
SDS-PAGE
Unit / Price
0.01 mg (E-Coli)  /  $195 +1 FREE 8GB USB
0.01 mg (Yeast)  /  $195 +1 FREE 8GB USB
0.05 mg (Yeast)  /  $255 +1 FREE 8GB USB
0.05 mg (E-Coli)  /  $255 +1 FREE 8GB USB
0.1 mg (Yeast)  /  $415 +1 FREE 8GB USB
0.1 mg (E-Coli)  /  $420 +1 FREE 8GB USB
0.2 mg (Yeast)  /  $660 +1 FREE 8GB USB
0.2 mg (E-Coli)  /  $665 +1 FREE 8GB USB
0.05 mg (Baculovirus)  /  $930 +1 FREE 8GB USB
0.5 mg (Yeast)  /  $1,090 +1 FREE 8GB USB
0.5 mg (E-Coli)  /  $1,090 +1 FREE 8GB USB
0.05 mg (Mammalian-Cell)  /  $1,170 +1 FREE 8GB USB
0.1 mg (Baculovirus)  /  $1,175 +1 FREE 8GB USB
0.5 mg (Baculovirus)  /  $1,695 +2 FREE 8GB USB
1 mg (E-Coli)  /  $1,725 +2 FREE 8GB USB
1 mg (Yeast)  /  $1,730 +2 FREE 8GB USB
0.1 mg (Mammalian-Cell)  /  $1,780 +2 FREE 8GB USB
1 mg (Baculovirus)  /  $2,260 +2 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Type III iodothyronine deiodinase (Dio3), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Rat Type III iodothyronine deiodinase (Dio3), partial

 Product Synonym Names    5DIII; DIOIII; Type 3 DI; Type-III 5'-deiodinase
 Product Gene Name   

Dio3 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence Positions    37-278aa (Missing the aa "U" at the 144 site. This is the complete extracellular domain before UniProt record was updated. Current UniProt #P49897. A cysteine mutant or serine mutant of MBS1116133 is also available upon request)
 Sequence    DFLCIRKHFL RRRHPDHPEP EVELNSEGEE MPPDDPPICV SDDNRLCTLA SLKAVWHGQK LDFFKQAHEG GPAPNSEVVR PDGFQSQRIL DYAQGTRPLV LNFGSCTPPF MARMSAFQRL VTKYQRDVDF LIIYIEEAHP SDGWVTTDSP YVIPQHRSLE DRVSAARVLQ QGAPGCALVL DTMANSSSSA YGAYFERLYV IQSGTIMYQG GRGPDGYQVS ELRTWLERYD EQLHGTRPRR L
 3D Structure    ModBase 3D Structure for P49897
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 Host    E Coli or Yeast or Baculovirus or Mammalian Cell
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 Purity/Purification    Greater than 90% as determined by SDS-PAGE. (lot specific)
 Form/Format    Liquid containing glycerol
 Tag Information    This protein contains an N-terminal tag and may also contain a C-terminal Myc-tag. N-terminal host tags may vary (His, His-SUMO, His-B2M, GST). Tag types are determined by various factors including tag-protein stability and, therefore, are subject to change; please inquire for tag information.
 Sterility    Sterile filter available upon request.
 Endotoxin    Low endotoxin available upon request.
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 Preparation and Storage    Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of Dio3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for Dio3 recombinant protein

   Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing prature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during embryological development.
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 SDS-PAGE of Dio3 recombinant protein    Dio3 recombinant protein SDS-PAGE image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
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NCBI/Uniprot data below describe general gene information for Dio3. It may not necessarily be applicable to this product.
 NCBI GI #    255982585
 NCBI GeneID    29475
 NCBI Accession #    NP_058906.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_017210.3 [Other Products]
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 UniProt Primary Accession #    P49897 [Other Products]
 UniProt Related Accession #    P49897 [Other Products]
 Molecular Weight    31.5kD
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 NCBI Official Full Name    thyroxine 5-deiodinase
 NCBI Official Synonym Full Names    deiodinase, iodothyronine, type III
 NCBI Official Symbol    Dio3 [Similar Products]
 NCBI Official Synonym Symbols   
5DIII; DIOIII
[Similar Products]
 NCBI Protein Information    thyroxine 5-deiodinase
 UniProt Protein Name    Thyroxine 5-deiodinase
 UniProt Synonym Protein Names   
5DIII; DIOIII; Type 3 DI; Type III iodothyronine deiodinase
 Protein Family    Thyroxine 5-deiodinase
 UniProt Gene Name    Dio3 [Similar Products]
 UniProt Synonym Gene Names    Itdi3; Txdi3 [Similar Products]
 UniProt Entry Name    IOD3_RAT
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 NCBI Summary for Dio3    The protein encoded by this intronless gene belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. This enzyme is highly expressed in the pregnant uterus, placenta, fetal and neonatal tissues, suggesting that it plays an essential role in the regulation of thyroid hormone inactivation during embryological development. It contains a selenocysteine (Sec) residue, which is essential for efficient enzyme activity. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jul 2008]
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 UniProt Comments for Dio3    DIO3: Responsible for the deiodination of T4 (3,5,3',5'- tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during embryological development. Belongs to the iodothyronine deiodinase family.

Protein type: EC 1.97.1.11; Oxidoreductase; Membrane protein, integral

Cellular Component: endosome membrane; integral to membrane; plasma membrane

Molecular Function: thyroxine 5'-deiodinase activity; thyroxine 5-deiodinase activity

Biological Process: hormone biosynthetic process; positive regulation of multicellular organism growth; response to hypoxia; thyroid hormone catabolic process; thyroid hormone metabolic process
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Product References and Citations for Dio3 recombinant protein

   Genome sequence of the Brown Norway rat yields insights into mammalian evolution.Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S.Nature 428:493-521(2004) Cloning and expression of a cDNA for a mammalian type III iodothyronine deiodinase.Croteau W., Whittemore S.L., Schneider M.J., St Germain D.L.J. Biol. Chem. 270:16569-16575(1995)
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 Research Articles on Dio3    1. findings suggest that translational repression of Dio3 is an important determinant of the reduced D3 protein expression following liver damage
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with Dio3 recombinant proteinDiseases associated with Dio3 recombinant protein
 Products by Pathway  Pathway Diagram
 Selenium Metabolism Selenoproteins Pathway antibodies  Selenium Metabolism Selenoproteins Pathway Diagram
 Thyroid Hormone Signaling Pathway antibodies  Thyroid Hormone Signaling Pathway Diagram
 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >9 publications with Dio3 and Liver Diseases
 Disease Models, Animal Antibodies  >8 publications with Dio3 and Disease Models, Animal
 Liver Neoplasms Antibodies  >7 publications with Dio3 and Liver Neoplasms
 Heart Diseases Antibodies  >6 publications with Dio3 and Heart Diseases
 Hypertrophy Antibodies  >6 publications with Dio3 and Hypertrophy
 Carcinoma, Hepatocellular Antibodies  >5 publications with Dio3 and Carcinoma, Hepatocellular
 Prenatal Exposure Delayed Effects Antibodies  >5 publications with Dio3 and Prenatal Exposure Delayed Effects
 Prenatal Injuries Antibodies  >5 publications with Dio3 and Prenatal Injuries
 Neoplasms, Experimental Antibodies  >4 publications with Dio3 and Neoplasms, Experimental
 Cell Transformation, Neoplastic Antibodies  >4 publications with Dio3 and Cell Transformation, Neoplastic
Organs/Tissues associated with Dio3 recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >33 publications with Dio3 and Liver
 Embryonic Tissue Antibodies  >17 publications with Dio3 and Embryonic Tissue
 Adipose Tissue Antibodies  >9 publications with Dio3 and Adipose Tissue
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