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IOD3 recombinant protein :: Type III iodothyronine deiodinase Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS9422645 IOD3 recombinant protein
Unit / Price
0.01 mg  /  $230 +1 FREE 8GB USB
0.05 mg  /  $290 +1 FREE 8GB USB
0.1 mg  /  $455 +1 FREE 8GB USB
0.2 mg  /  $705 +1 FREE 8GB USB
0.5 mg  /  $1,145 +1 FREE 8GB USB
1 mg  /  $1,800 +2 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Type III iodothyronine deiodinase (IOD3), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Rat Type III iodothyronine deiodinase

 Product Synonym Names    5DIII; DIOIII; Type 3 DI; Type-III 5'-deiodinase
 Product Gene Name   

IOD3 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence Positions    : 37-271aa(U132S); Partial, provide the complete extracellular domain.
 Sequence    DFQCIRRRVL LTAREESTAE HEDPPLCVSD SNRMCTVESL RAVWHGQKLD YFKSAHLGCS APNTEVVMLE GRRLCKILDF SQGKRPLVVN FGSCTSPPFM ARLQAYRRLA AQH VGIAD FLLVYIEEAH PSDGWLSTDA SYQIPQHQCL QDRLAAAQLM LQGAPGCRVV VDTMDNSSNA AYGAYFERLY IVLEGKVVYQ GGRGPEGYKI SELRMWLEQY QQGLMGTK GSGQVVIQV
 3D Structure    ModBase 3D Structure for P49897
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 Host    Yeast
 Species Reactivity    Rat
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 Purity/Purification    Greater than 90% as determined by SDS-PAGE.
 Form/Format    20mM Tris-HCl based buffer, pH8.0
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 Tag information    EP, YP, BP, MP: Tag type will be determined during the manufacturing process.

The expected tag for each expression system is list as follows:
YP: N-terminal 6xHis-tagged; EP, BP, MP: N-terminal 10xHis-tagged and C-terminal Myc-tagged
 Tag Info    N-terminal 6xHis-tagged
 Note    The amino acid at position 132 is originally a "U", which has already been mutated to be "S" in the above sequence. This is due to the "U", or selenocysteine, which is encoded by UGA, is normally used as a stop codon in prokaryotic cells. Thus leading to early termination of translation. So we have substituted this "U" to simply be an "S" so that translation can proceed. Changing to cysteine may result in undesired disulfide bond formation, so serine is chosen since it is structurally related to both selenocysteine and cysteine.
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 Preparation and Storage    Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C. Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.
 Other Notes    Small volumes of IOD3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for IOD3 recombinant protein

   Responsible for the deiodination of T4 (3, 5, 3', 5'-tetraiodothyronine) into RT3 (3, 3', 5'-triiodothyronine) and of T3 (3, 5, 3'-triiodothyronine) into T2 (3, 3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing prature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during bryological development.
NCBI/Uniprot data below describe general gene information for IOD3. It may not necessarily be applicable to this product.
 NCBI GI #    255982585
 NCBI GeneID    29475
 NCBI Accession #    NP_058906.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_017210.4 [Other Products]
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 UniProt Primary Accession #    P49897 [Other Products]
 UniProt Related Accession #    P49897 [Other Products]
 Molecular Weight    29.4kD
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 NCBI Official Full Name    thyroxine 5-deiodinase
 NCBI Official Synonym Full Names    iodothyronine deiodinase 3
 NCBI Official Symbol    Dio3 [Similar Products]
 NCBI Official Synonym Symbols   
5DIII; DIOIII
[Similar Products]
 NCBI Protein Information    thyroxine 5-deiodinase
 UniProt Protein Name    Thyroxine 5-deiodinase
 UniProt Synonym Protein Names   
5DIII; DIOIII; Type 3 DI; Type III iodothyronine deiodinase
 UniProt Gene Name    Dio3 [Similar Products]
 UniProt Synonym Gene Names    Itdi3; Txdi3 [Similar Products]
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 NCBI Summary for IOD3    This is an intronless, imprinted gene that is preferentially expressed from the paternal allele. The encoded protein belongs to the iodothyronine deiodinase family, and catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5' triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. It is highly expressed in placenta, fetal and neonatal tissues, and thought to prevent premature exposure of developing fetal tissues to adult levels of thyroid hormones. It thus plays a critical role in mammalian development by regulating circulating fetal thyroid hormone concentration. This protein is a selenoprotein, containing the rare selenocysteine (Sec) amino acid at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jun 2016]
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 UniProt Comments for IOD3    DIO3: Responsible for the deiodination of T4 (3,5,3',5'- tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during embryological development. Belongs to the iodothyronine deiodinase family.

Protein type: EC 1.97.1.11; Membrane protein, integral; Oxidoreductase

Chromosomal Location of Human Ortholog: 6q32

Molecular Function: thyroxine 5'-deiodinase activity; thyroxine 5-deiodinase activity

Biological Process: apoptosis; positive regulation of multicellular organism growth; response to hypoxia; retinal cone cell development; thyroid hormone catabolic process; thyroid hormone metabolic process
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 Research Articles on IOD3    1. findings suggest that translational repression of Dio3 is an important determinant of the reduced D3 protein expression following liver damage
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with IOD3 recombinant proteinDiseases associated with IOD3 recombinant protein
 Products by Pathway  Pathway Diagram
 Selenium Metabolism Selenoproteins Pathway antibodies  Selenium Metabolism Selenoproteins Pathway Diagram
 Thyroid Hormone Signaling Pathway antibodies  Thyroid Hormone Signaling Pathway Diagram
 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >11 publications with IOD3 and Disease Models, Animal
 Liver Diseases Antibodies  >11 publications with IOD3 and Liver Diseases
 Heart Diseases Antibodies  >9 publications with IOD3 and Heart Diseases
 Liver Neoplasms Antibodies  >8 publications with IOD3 and Liver Neoplasms
 Chromosome Aberrations Antibodies  >7 publications with IOD3 and Chromosome Aberrations
 Hypertrophy Antibodies  >7 publications with IOD3 and Hypertrophy
 Prenatal Exposure Delayed Effects Antibodies  >5 publications with IOD3 and Prenatal Exposure Delayed Effects
 Prenatal Injuries Antibodies  >5 publications with IOD3 and Prenatal Injuries
 Carcinoma, Hepatocellular Antibodies  >5 publications with IOD3 and Carcinoma, Hepatocellular
 Inflammation Antibodies  >4 publications with IOD3 and Inflammation
Organs/Tissues associated with IOD3 recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >41 publications with IOD3 and Liver
 Embryonic Tissue Antibodies  >19 publications with IOD3 and Embryonic Tissue
 Adipose Tissue Antibodies  >10 publications with IOD3 and Adipose Tissue
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