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ISG15 recombinant protein :: Ubiquitin-like protein ISG15 Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS717412
Unit / Price
0.01 mg  /  $110 +1 FREE 8GB USB
0.05 mg  /  $190 +1 FREE 8GB USB
0.1 mg  /  $285 +1 FREE 8GB USB
0.2 mg  /  $460 +1 FREE 8GB USB
0.5 mg  /  $750 +1 FREE 8GB USB
1 mg  /  $1,180 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Ubiquitin-like protein ISG15, Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Human Ubiquitin-like protein ISG15

 Product Synonym Names    Interferon-induced 15 kDa proteinInterferon-induced 17 kDa protein; IP17Ubiquitin cross-reactive protein
 Product Gene Name   

ISG15 recombinant protein

[Similar Products]
 Product Synonym Gene Name    G1P2; UCRP [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 MBS717412 COA    COA PDF
 Sequence Positions    2-157. Full length.
 3D Structure    ModBase 3D Structure for Q64339
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 Host    E Coli
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 Purity/Purification    Greater than 90% as determined by SDS-PAGE. (lot specific)
 Form/Format    Liquid containing glycerol
 Tag Information    This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
 Sterility    Sterile filter available upon request.
 Endotoxin    Low endotoxin available upon request.
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 Preparation and Storage    Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of ISG15 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for ISG15 recombinant protein

   Ubiquitin-like protein which plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. ISGylation involves a cascade of enzymatic reactions involving E1, E2, and E3 enzymes which catalyze the conjugation of ISG15 to a lysine residue in the target protein. Its target proteins include SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, TRIM25, STAT5A, MAPK1/ERK2 and globin. Can also isgylate: DDX58/RIG-I which inhibits its function in antiviral signaling response and EIF4E2 which enhances its cap structure-binding activity and translation-inhibition activity. Exhibits antiviral activity towards both DNA and RNA viruses, including influenza A and B virus, sindbis virus (SV) and herpes simplex type-1 (HHV-1). Plays a significant role in the control of neonatal Chikungunya virus (CHIKV) infection by acting as a putative immunomodulator of proinflammatory cytokines. Protects mice against the consequences of Chikungunya virus infection by downregulating the pathogenic cytokine response, often denoted as the cytokine storm. Plays a role in erythroid differentiation. The secreted form of ISG15 can: induce natural killer cell proliferation, act as a chotactic factor for neutrophils and act as a IFN-gamma-inducing cytokine playing an essential role in antimycobacterial immunity
 Product Categories/Family for ISG15 recombinant protein    Immunology
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 SDS-PAGE of ISG15 recombinant protein    ISG15 recombinant protein SDS-PAGE image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
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NCBI/Uniprot data below describe general gene information for ISG15. It may not necessarily be applicable to this product.
 NCBI GI #    226874851
 NCBI GeneID    100038882
 NCBI Accession #    NP_056598.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_015783.3 [Other Products]
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 UniProt Primary Accession #    Q64339 [Other Products]
 UniProt Secondary Accession #    Q8K0H3 [Other Products]
 UniProt Related Accession #    Q64339 [Other Products]
 Molecular Weight    44.4kD
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 NCBI Official Full Name    ubiquitin-like protein ISG15
 NCBI Official Synonym Full Names    ISG15 ubiquitin-like modifier
 NCBI Official Symbol    Isg15 [Similar Products]
 NCBI Official Synonym Symbols   
G1p2; IP17; Irfp; UCRP; IGI15
[Similar Products]
 NCBI Protein Information    ubiquitin-like protein ISG15
 UniProt Protein Name    Ubiquitin-like protein ISG15
 UniProt Synonym Protein Names   
Interferon-induced 15 kDa protein; Interferon-induced 17 kDa protein; IP17; Ubiquitin cross-reactive protein
 Protein Family    Ubiquitin-like protein
 UniProt Gene Name    Isg15 [Similar Products]
 UniProt Synonym Gene Names    G1p2; Ucrp; IP17 [Similar Products]
 UniProt Entry Name    ISG15_MOUSE
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 UniProt Comments for ISG15    ISG15: Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections. Interacts with, and is conjugated to its targets by the UBE1L (E1 enzyme) and UBE2E2 (E2 enzyme) (Probable). Interaction with influenza B NS1 protein inhibits this conjugation. By type I interferons. Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons.

Protein type: Ubiquitin-like modifier

Cellular Component: cytoplasm; extracellular region

Molecular Function: protein binding; protein tag

Biological Process: defense response to bacterium; defense response to virus; ISG15-protein conjugation; modification-dependent protein catabolic process; negative regulation of protein ubiquitination; negative regulation of viral genome replication; positive regulation of erythrocyte differentiation; regulation of interferon-gamma production
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Product References and Citations for ISG15 recombinant protein

   Nucleotide sequence and deduced amino acid sequence of a cDNA encoding an interferon-induced mouse 15-KDa protein.Fahey D. Sequence differences between murine ISG15, human ISG15 and ubiquitin a functional divergence from ubiquitin.Garlie N.K., Haas A.L., D'Cunha J., Schilling D., Knight E. Jr., Borden E.C. Serpin 2a is induced in activated macrophages and conjugates to a ubiquitin homolog.Hamerman J.A., Hayashi F., Schroeder L.A., Gygi S.P., Haas A.L., Hampson L., Coughlin P., Aebersold R., Aderem A.J. Immunol. 168:2415-2423(2002) Identification of a ubiquitin family protein as a novel neutrophil chemotactic factor.Owhashi M., Taoka Y., Ishii K., Nakazawa S., Uemura H., Kambara H.Biochem. Biophys. Res. Commun. 309:533-539(2003) High-throughput immunoblotting. Ubiquitin-like protein ISG15 modifies key regulators of signal transduction.Malakhov M.P., Kim K.I., Malakhova O.A., Jacobs B.S., Borden E.C., Zhang D.-E.J. Biol. Chem. 278:16608-16613(2003) Identification of interferon-stimulated gene 15 as an antiviral molecule during Sindbis virus infection in vivo.Lenschow D.J., Giannakopoulos N.V., Gunn L.J., Johnston C., O'Guin A.K., Schmidt R.E., Levine B., Virgin H.W. IVJ. Virol. 79:13974-13983(2005) Negative regulation of ISG15 E3 ligase EFP through its autoISGylation.Zou W., Wang J., Zhang D.-E.Biochem. Biophys. Res. Commun. 354:321-327(2007) ISG15 modification of the eIF4E cognate 4EHP enhances cap structure-binding activity of 4EHP.Okumura F., Zou W., Zhang D.E.Genes Dev. 21:255-260(2007) IFN-stimulated gene 15 functions as a critical antiviral molecule against influenza, herpes, and Sindbis viruses.Lenschow D.J., Lai C., Frias-Staheli N., Giannakopoulos N.V., Lutz A., Wolff T., Osiak A., Levine B., Schmidt R.E., Garcia-Sastre A., Leib D.A., Pekosz A., Knobeloch K.P., Horak I., Virgin H.W. IVProc. Natl. Acad. Sci. U.S.A. 104:1371-1376(2007) Nitrosylation of ISG15 prevents the disulfide bond-mediated dimerization of ISG15 and contributes to effective ISGylation.Okumura F., Lenschow D.J., Zhang D.E.J. Biol. Chem. 283:24484-24488(2008) Negative feedback regulation of RIG-I-mediated antiviral signaling by interferon-induced ISG15 conjugation.Kim M.J., Hwang S.Y., Imaizumi T., Yoo J.Y.J. Virol. 82:1474-1483(2008) Emerging role of ISG15 in antiviral immunity.Skaug B., Chen Z.J.Cell 143:187-190(2010) ISG15 modulates development of the erythroid lineage.Maragno A.L., Pironin M., Alcalde H., Cong X., Knobeloch K.P., Tangy F., Zhang D.E., Ghysdael J., Quang C.T.PLoS ONE 6:E26068-E26068(2011) ISG15 is critical in the control of Chikungunya virus infection independent of UbE1L mediated conjugation.Werneke S.W., Schilte C., Rohatgi A., Monte K.J., Michault A., Arenzana-Seisdedos F., Vanlandingham D.L., Higgs S., Fontanet A., Albert M.L., Lenschow D.J.PLoS Pathog. 7:E1002322-E1002322(2011) Mycobacterial disease and impaired IFN-gamma immunity in humans with inherited ISG15 deficiency.Bogunovic D., Byun M., Durfee L.A., Abhyankar A., Sanal O., Mansouri D., Salem S., Radovanovic I., Grant A.V., Adimi P., Mansouri N., Okada S., Bryant V.L., Kong X.F., Kreins A., Velez M.M., Boisson B., Khalilzadeh S., Ozcelik U., Darazam I.A., Schoggins J.W., Rice C.M., Al-Muhsen S., Behr M., Vogt G., Puel A., Bustamante J., Gros P., Huibregtse J.M., Abel L., Boisson-Dupuis S., Casanova J.L.Science 337:1684-1688(2012)
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 Research Articles on ISG15    1. Kruppel-like factor 9 (KLF9) is a haploinsufficient tumor suppressor in the ApcMin/+ mouse colon by suppressing expression of ISG15, an apoptosis-inhibiting cytokine.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ISG15 recombinant proteinPathways associated with ISG15 recombinant protein
 Reference Product  PubMed Publications
 Stat1 recombinant protein  >70 publications with ISG15 and Stat1
 Usp18 recombinant protein  >69 publications with ISG15 and Usp18
 Ifit1 recombinant protein  >32 publications with ISG15 and Ifit1
 Mx2 recombinant protein  >18 publications with ISG15 and Mx2
 Ddx58 recombinant protein  >18 publications with ISG15 and Ddx58
 Ube2l6 recombinant protein  >14 publications with ISG15 and Ube2l6
 Herc6 recombinant protein  >7 publications with ISG15 and Herc6
 Nedd4 recombinant protein  >3 publications with ISG15 and Nedd4
 Uba7 recombinant protein  >2 publications with ISG15 and Uba7
 Ube2n recombinant protein  >1 publications with ISG15 and Ube2n
 Products by Pathway  Pathway Diagram
 Antiviral Mechanism By IFN-stimulated Genes Pathway antibodies  Antiviral Mechanism By IFN-stimulated Genes Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 DNA Damage Bypass Pathway antibodies  DNA Damage Bypass Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 ISG15 Antiviral Mechanism Pathway antibodies  ISG15 Antiviral Mechanism Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Interferon Signaling Pathway antibodies  Interferon Signaling Pathway Diagram
 RIG-I-like Receptor Signaling Pathway antibodies  RIG-I-like Receptor Signaling Pathway Diagram
 RIG-I-like Receptor Signaling Pathway antibodies  RIG-I-like Receptor Signaling Pathway Diagram
 Termination Of Translesion DNA Synthesis Pathway antibodies  Termination Of Translesion DNA Synthesis Pathway Diagram
Diseases associated with ISG15 recombinant proteinOrgans/Tissues associated with ISG15 recombinant protein
 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >54 publications with ISG15 and Liver Diseases
 Inflammation Antibodies  >37 publications with ISG15 and Inflammation
 Influenza, Human Antibodies  >30 publications with ISG15 and Influenza, Human
 Necrosis Antibodies  >28 publications with ISG15 and Necrosis
 Skin Diseases Antibodies  >26 publications with ISG15 and Skin Diseases
 Kidney Diseases Antibodies  >19 publications with ISG15 and Kidney Diseases
 Cardiovascular Diseases Antibodies  >14 publications with ISG15 and Cardiovascular Diseases
 Lung Diseases Antibodies  >14 publications with ISG15 and Lung Diseases
 Cell Transformation, Neoplastic Antibodies  >14 publications with ISG15 and Cell Transformation, Neoplastic
 Breast Neoplasms Antibodies  >14 publications with ISG15 and Breast Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >28 publications with ISG15 and Brain
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