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Rag2 recombinant protein :: V (D)J recombination-activating protein 2 Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS1001277 Rag2 recombinant protein
Unit / Price
0.05 mg (E-Coli)  /  $950 +1 FREE 8GB USB
0.05 mg (Baculovirus)  /  $1,150 +1 FREE 8GB USB
0.2 mg (E-Coli)  /  $1,275 +1 FREE 8GB USB
0.2 mg (Yeast)  /  $1,380 +1 FREE 8GB USB
0.05 mg (Mammalian-Cell)  /  $1,400 +1 FREE 8GB USB
0.5 mg (E-Coli)  /  $1,405 +1 FREE 8GB USB
0.1 mg (Baculovirus)  /  $1,455 +1 FREE 8GB USB
0.5 mg (Yeast)  /  $1,565 +1 FREE 8GB USB
1 mg (E-Coli)  /  $1,995 +2 FREE 8GB USB
0.5 mg (Baculovirus)  /  $2,085 +2 FREE 8GB USB
0.1 mg (Mammalian-Cell)  /  $2,110 +2 FREE 8GB USB
1 mg (Yeast)  /  $2,380 +2 FREE 8GB USB
1 mg (Baculovirus)  /  $2,740 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

V (D)J recombination-activating protein 2 (Rag2), Recombinant Protein

 Also Known As   

Recombinant Mouse V (D)J recombination-activating protein 2

 Product Gene Name   

Rag2 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence Positions    1-527aa, full length protein
 Sequence    MSLQMVTVGH NIALIQPGFS LMNFDGQVFF FGQKGWPKRS CPTGVFHFDI KQNHLKLKPA IFSKDSCYLP PLRYPATCSY KGSIDSDKHQ YIIHGGKTPN NELSDKIYIM SVACKNNKKV TFRCTEKDLV GDVPEPRYGH SIDVVYSRGK SMGVLFGGRS YMPSTQRTTE KWNSVADCLP HVFLIDFEFG CATSYILPEL QDGLSFHVSI ARNDTVYILG GHSLASNIRP ANLYRIRVDL PLGTPAVNCT VLPGGISVSS AILTQTNNDE FVIVGGYQLE NQKRMVCSLV SLGDNTIEIS EMETPDWTSD IKHSKIWFGS NMGNGTIFLG IPGDNKQAMS EAFYFYTLRC SEEDLSEDQK IVSNSQTSTE DPGDSTPFED SEEFCFSAEA TSFDGDDEFD TYNEDDEDDE SVTGYWITCC PTCDVDINTW VPFYSTELNK PAMIYCSHGD GHWVHAQCMD LEERTLIHLS EGSNKYYCNE HVQIARALQT PKRNPPLQKP PMKSLHKKGS GKVLTPAKKS FLRRLFD
 3D Structure    ModBase 3D Structure for P21784
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 Host    E Coli or Yeast or Baculovirus or Mammalian Cell
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 Purity/Purification    >85% (SDS-PAGE) (lot specific)
 Form/Format    Liquid containing glycerol
 Tag Information    This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
 Sterility    Sterile filter available upon request.
 Endotoxin    Low endotoxin available upon request.
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 Species    Mouse
 Storage Buffer    Tris-based buffer, 50% glycerol
 Tag Information    Tag type will be determined during the manufacturing process
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 Preparation and Storage    Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of Rag2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for Rag2 recombinant protein

   This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms.
NCBI/Uniprot data below describe general gene information for Rag2. It may not necessarily be applicable to this product.
 NCBI GI #    6677661
 NCBI GeneID    19374
 NCBI Accession #    NP_033046.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_009020.3 [Other Products]
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 UniProt Primary Accession #    P21784 [Other Products]
 UniProt Related Accession #    P21784 [Other Products]
 Molecular Weight    59,074 Da
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 NCBI Official Full Name    V(D)J recombination-activating protein 2
 NCBI Official Synonym Full Names    recombination activating gene 2
 NCBI Official Symbol    Rag2 [Similar Products]
 NCBI Official Synonym Symbols   
Rag-2
[Similar Products]
 NCBI Protein Information    V(D)J recombination-activating protein 2
 UniProt Protein Name    V(D)J recombination-activating protein 2
 Protein Family    V(D)J recombination-activating protein
 UniProt Gene Name    Rag2 [Similar Products]
 UniProt Synonym Gene Names    Rag-2; RAG-2 [Similar Products]
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 UniProt Comments for Rag2    Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3.
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 Research Articles on Rag2    1. This work provides important mechanistic insight into how spatiotemporal expression of the Rag genes is tightly controlled during B lymphocyte development to prevent mistimed dsDNA breaks and their deleterious consequences.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Rag2 recombinant proteinPathways associated with Rag2 recombinant protein
 Reference Product  PubMed Publications
 Rag1 recombinant protein  >470 publications with Rag2 and Rag1
 Cd40 recombinant protein  >37 publications with Rag2 and Cd40
 Hmgb1 recombinant protein  >31 publications with Rag2 and Hmgb1
 Pax5 recombinant protein  >26 publications with Rag2 and Pax5
 Prkdc recombinant protein  >11 publications with Rag2 and Prkdc
 Ccr9 recombinant protein  >5 publications with Rag2 and Ccr9
 Ptprc recombinant protein  >4 publications with Rag2 and Ptprc
 Lef1 recombinant protein  >1 publications with Rag2 and Lef1
 Products by Pathway  Pathway Diagram
 FoxO Signaling Pathway antibodies  FoxO Signaling Pathway Diagram
 Primary Immunodeficiency Pathway antibodies  Primary Immunodeficiency Pathway Diagram
 Primary Immunodeficiency Pathway antibodies  Primary Immunodeficiency Pathway Diagram
Diseases associated with Rag2 recombinant proteinOrgans/Tissues associated with Rag2 recombinant protein
 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >532 publications with Rag2 and Immune System Diseases
 Disease Models, Animal Antibodies  >336 publications with Rag2 and Disease Models, Animal
 Inflammation Antibodies  >216 publications with Rag2 and Inflammation
 Necrosis Antibodies  >80 publications with Rag2 and Necrosis
 Kidney Diseases Antibodies  >37 publications with Rag2 and Kidney Diseases
 Hyperplasia Antibodies  >31 publications with Rag2 and Hyperplasia
 Hepatitis Antibodies  >24 publications with Rag2 and Hepatitis
 Metaplasia Antibodies  >19 publications with Rag2 and Metaplasia
 Atherosclerosis Antibodies  >16 publications with Rag2 and Atherosclerosis
 Abnormalities, Multiple Antibodies  >15 publications with Rag2 and Abnormalities, Multiple
 Organ/Tissue Name  Pubmed Publications
 Thymus Antibodies  >285 publications with Rag2 and Thymus
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