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VEGF recombinant protein

Scan QR to view Datasheet Catalog #    MBS232360 VEGF recombinant protein
Unit / Price
0.01 mg  /  $440 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

VEGF, Recombinant Protein

 Also Known As   


 Product Gene Name   

VEGF recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 3D Structure    ModBase 3D Structure for Q00731
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 Purity/Purification    >98% by SDS page and HPLC analysis
 Form/Format    Purified Recombinant Protein - lyophilised
 Concentration    Approximate Protein Concentration: 0.1 mg/ml after reconstitution (lot specific)
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 Reconstitution    Reconstitute with 0.1ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide
 Buffer Solution    0.1% Trifluoroacetic acid.
 Target Species    Mouse
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 Preparation and Storage    Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 3 months from date of reconstitution.
 Other Notes    Small volumes of VEGF recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for VEGF recombinant protein

   Murine Vascular Endothelial Growth Factor (VEGF) is a homodimeric protein, where each subunit is 165 amino acids in length. Murine VEGF is expressed by various vascularised tissues and is reported to stimulate endothelial cell growth and angiogenesis.
 Applications Tested/Suitable for VEGF recombinant protein   

ELISA (EIA), Functional Assays (FN), Western Blot (WB)

 Application Notes for VEGF recombinant protein    ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4ng/well;
Functional Assays: Minimum Dilution: 1.0ng/ml; Maximum Dilution: 5.0ng/ml;
Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane
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NCBI/Uniprot data below describe general gene information for VEGF. It may not necessarily be applicable to this product.
 NCBI GI #    160358799
 NCBI GeneID    22339
 NCBI Accession #    NP_001020421.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_001025250.3 [Other Products]
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 UniProt Primary Accession #    Q00731 [Other Products]
 UniProt Secondary Accession #    O70123; Q66S31; Q6GT23; Q6WZL9 [Other Products]
 UniProt Related Accession #    Q00731 [Other Products]
 Molecular Weight    Protein Molecular Weight: 39kD (homodimer of 165 amino acid sequence)
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 NCBI Official Full Name    vascular endothelial growth factor A isoform 1
 NCBI Official Synonym Full Names    vascular endothelial growth factor A
 NCBI Official Symbol    Vegfa [Similar Products]
 NCBI Official Synonym Symbols   
Vpf; Vegf
[Similar Products]
 NCBI Protein Information    vascular endothelial growth factor A; vascular permeability factor
 UniProt Protein Name    Vascular endothelial growth factor A
 UniProt Synonym Protein Names   
Vascular permeability factor; VPF
 Protein Family    VEGF coregulated chemokine
 UniProt Gene Name    Vegfa [Similar Products]
 UniProt Synonym Gene Names    Vegf; VEGF-A; VPF [Similar Products]
 UniProt Entry Name    VEGFA_MOUSE
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 NCBI Summary for VEGF    This gene product is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Also, alternative translation initiation from non-AUG (CUG) and AUG start sites in some transcript variants, give rise to additional isoforms. The expression of some isoforms derived from AUG start codon is affected by a small upstream open reading frame, which is located within an internal ribosome entry site. [provided by RefSeq, Nov 2013]
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 UniProt Comments for VEGF    VEGF: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the PDGF/VEGF growth factor family. 13 isoforms of the human protein are produced by alternative promoter.

Protein type: Cytokine; Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Cellular Component: extracellular space; cell surface; membrane; cytoplasm; extracellular region; plasma membrane; basement membrane; secretory granule

Molecular Function: heparin binding; identical protein binding; protein binding; protein homodimerization activity; growth factor activity; vascular endothelial growth factor receptor 2 binding; extracellular matrix binding; protein heterodimerization activity; fibronectin binding; platelet-derived growth factor receptor binding; cytokine activity; vascular endothelial growth factor receptor 1 binding; receptor agonist activity; vascular endothelial growth factor receptor binding; chemoattractant activity; receptor binding

Biological Process: heart morphogenesis; positive regulation of cell adhesion; macrophage differentiation; positive regulation of positive chemotaxis; multicellular organismal development; cell maturation; positive regulation of receptor internalization; basophil chemotaxis; positive regulation of MAP kinase activity; regulation of cell shape; positive chemotaxis; positive regulation of mesenchymal cell proliferation; mesoderm development; negative regulation of neuron apoptosis; kidney development; positive regulation of neuroblast proliferation; nervous system development; T-helper 1 type immune response; positive regulation of signal transduction; monocyte differentiation; mRNA stabilization; positive regulation of blood vessel endothelial cell migration; activation of CREB transcription factor; positive regulation of protein amino acid autophosphorylation; positive regulation of vascular permeability; regulation of endothelial cell differentiation; regulation of transcription from RNA polymerase II promoter; patterning of blood vessels; positive regulation of angiogenesis; positive regulation of peptidyl-tyrosine phosphorylation; eye photoreceptor cell development; camera-type eye morphogenesis; branching morphogenesis of a tube; cell migration during sprouting angiogenesis; cardiac muscle fiber development; positive regulation of cell division; activation of protein kinase activity; positive regulation of axon extension involved in axon guidance; endothelial cell migration; blood vessel morphogenesis; neuron development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; regulation of cGMP metabolic process; surfactant homeostasis; alveolus development; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; lactation; post-embryonic camera-type eye development; positive regulation of smooth muscle cell proliferation; negative regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; positive regulation of vascular endothelial growth factor receptor signaling pathway; induction of positive chemotaxis; vasculature development; ovarian follicle development; positive regulation of focal adhesion formation; epithelial cell differentiation; lymphangiogenesis; positive regulation of cell proliferation; negative regulation of programmed cell death; angiogenesis; negative regulation of bone resorption; cell differentiation; blood vessel development; cell migration; in utero embryonic development; lumen formation; positive regulation of cell motility; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein kinase B signaling cascade; cell proliferation; positive regulation of protein complex assembly; response to hypoxia; artery morphogenesis; blood vessel remodeling; positive regulation of protein amino acid phosphorylation; sprouting angiogenesis; negative regulation of cell-cell adhesion; vascular endothelial growth factor receptor signaling pathway; growth; positive regulation of cell migration; lung development
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 Research Articles on VEGF    1. of radiation. Data indicate that anti-VEGF (vascular endothelial growth factor A) antibodies slowed the progression of radiation necrosis in irradiated brain tissue.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with VEGF recombinant proteinDiseases associated with VEGF recombinant protein
 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Cellular Response To Hypoxia Pathway antibodies  Cellular Response To Hypoxia Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 EPH-Ephrin Signaling Pathway antibodies  EPH-Ephrin Signaling Pathway Diagram
 EPHA-mediated Growth Cone Collapse Pathway antibodies  EPHA-mediated Growth Cone Collapse Pathway Diagram
 Disease Name  Pubmed Publications
 Neovascularization, Pathologic Antibodies  >1887 publications with VEGF and Neovascularization, Pathologic
 Retinal Diseases Antibodies  >1359 publications with VEGF and Retinal Diseases
 Macular Degeneration Antibodies  >922 publications with VEGF and Macular Degeneration
 Neoplasm Metastasis Antibodies  >480 publications with VEGF and Neoplasm Metastasis
 Choroidal Neovascularization Antibodies  >463 publications with VEGF and Choroidal Neovascularization
 Inflammation Antibodies  >440 publications with VEGF and Inflammation
 Diabetic Retinopathy Antibodies  >433 publications with VEGF and Diabetic Retinopathy
 Lung Diseases Antibodies  >393 publications with VEGF and Lung Diseases
 Macular Edema Antibodies  >321 publications with VEGF and Macular Edema
 Heart Diseases Antibodies  >268 publications with VEGF and Heart Diseases
Organs/Tissues associated with VEGF recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >2911 publications with VEGF and Blood
 Eye Antibodies  >1333 publications with VEGF and Eye
 Lung Antibodies  >512 publications with VEGF and Lung
 Muscle Antibodies  >447 publications with VEGF and Muscle
 Embryonic Tissue Antibodies  >403 publications with VEGF and Embryonic Tissue
 Heart Antibodies  >359 publications with VEGF and Heart
 Liver Antibodies  >340 publications with VEGF and Liver
 Kidney Antibodies  >324 publications with VEGF and Kidney
 Brain Antibodies  >295 publications with VEGF and Brain
 Skin Antibodies  >270 publications with VEGF and Skin
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