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VEGF165 recombinant protein

Scan QR to view Datasheet Catalog #    MBS204522
SDS-PAGE
Unit / Price
0.05 mg  /  $375 +1 FREE 8GB USB
0.25 mg  /  $925 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

VEGF165, Recombinant Protein

 Also Known As   

VEGF165, 207-370aa, Human, E Coli

 Product Synonym Names    Vascular endothelial growth factor A; VEGF; MVCD1; VPF
 Product Gene Name   

VEGF165 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQENPCGP CSERRKHLFV QDPQTCKCSC KNTDSRCKAR QLELNERTCR CDKPRR
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 Purity/Purification    > 90% by SDS - PAGE
 Form/Format    Liquid. 20mM Tris-HCl buffer (pH8.0) containing 2mM EDTA, 0.1mM PMSF, 1mM DTT, 10% glycerol
 Concentration    0.5 mg/ml (determined by Bradford assay) (lot specific)
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 Antigen Species    Human
 Expression System    E Coli
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 Preparation and Storage    Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of VEGF165 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for VEGF165 recombinant protein

   Vascular endothelial growth factor (VEGF) is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis. Recombinant human VEGF165 protein was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
 Product Categories/Family for VEGF165 recombinant protein    Hormones & Cytokines
 Applications Tested/Suitable for VEGF165 recombinant protein   

SDS-PAGE

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 SDS-PAGE of VEGF165 recombinant protein    VEGF165 recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for VEGF165. It may not necessarily be applicable to this product.
 NCBI GI #    76781483
 NCBI GeneID    7422
 NCBI Accession #    NP_001020539 [Other Products]
 NCBI GenBank Nucleotide #    NM_001025368.2 [Other Products]
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 UniProt Secondary Accession #    O60720; O75875; Q074Z4; Q16889; B5BU86; H0Y2S8; H0Y407; H0Y414; H0Y462; H0Y8N2; H3BLW7 [Other Products]
 UniProt Related Accession #    P15692 [Other Products]
 Molecular Weight    19.3 kDa (166aa), confirmed by MALDI-TOF
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 NCBI Official Full Name    vascular endothelial growth factor A isoform d
 NCBI Official Synonym Full Names    vascular endothelial growth factor A
 NCBI Official Symbol    VEGFA [Similar Products]
 NCBI Official Synonym Symbols   
VPF; VEGF; MVCD1
[Similar Products]
 NCBI Protein Information    vascular endothelial growth factor A; vascular permeability factor
 UniProt Protein Name    Vascular endothelial growth factor A
 UniProt Synonym Protein Names   
Vascular permeability factor; VPF
 UniProt Gene Name    VEGFA [Similar Products]
 UniProt Synonym Gene Names    VEGF; VEGF-A; VPF [Similar Products]
 UniProt Entry Name    VEGFA_HUMAN
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 NCBI Summary for VEGF165    This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq, Jul 2008]
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 UniProt Comments for VEGF165    Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
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Product References and Citations for VEGF165 recombinant protein

   Murphy J.F., et al. (2001) FASEB J. 15:1667-1669
Woolard J., et al. (2004) Cancer Res. 64:7822-7835
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 Research Articles on VEGF165    1. miR-185-mediated Vegfa targeting may be involved in breast cancer formation
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with VEGF165 recombinant proteinDiseases associated with VEGF165 recombinant protein
 Products by Pathway  Pathway Diagram
 Angiogenesis Pathway antibodies  Angiogenesis Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Cellular Response To Hypoxia Pathway antibodies  Cellular Response To Hypoxia Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 EPH-Ephrin Signaling Pathway antibodies  EPH-Ephrin Signaling Pathway Diagram
 Disease Name  Pubmed Publications
 Neovascularization, Pathologic Antibodies  >1866 publications with VEGF165 and Neovascularization, Pathologic
 Retinal Diseases Antibodies  >1342 publications with VEGF165 and Retinal Diseases
 Macular Degeneration Antibodies  >908 publications with VEGF165 and Macular Degeneration
 Neoplasm Metastasis Antibodies  >477 publications with VEGF165 and Neoplasm Metastasis
 Choroidal Neovascularization Antibodies  >457 publications with VEGF165 and Choroidal Neovascularization
 Inflammation Antibodies  >438 publications with VEGF165 and Inflammation
 Diabetic Retinopathy Antibodies  >432 publications with VEGF165 and Diabetic Retinopathy
 Lung Diseases Antibodies  >388 publications with VEGF165 and Lung Diseases
 Macular Edema Antibodies  >319 publications with VEGF165 and Macular Edema
 Heart Diseases Antibodies  >263 publications with VEGF165 and Heart Diseases
Organs/Tissues associated with VEGF165 recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >7559 publications with VEGF165 and Vascular
 Blood Antibodies  >2885 publications with VEGF165 and Blood
 Eye Antibodies  >1316 publications with VEGF165 and Eye
 Lung Antibodies  >505 publications with VEGF165 and Lung
 Bone Antibodies  >505 publications with VEGF165 and Bone
 Muscle Antibodies  >442 publications with VEGF165 and Muscle
 Embryonic Tissue Antibodies  >396 publications with VEGF165 and Embryonic Tissue
 Heart Antibodies  >356 publications with VEGF165 and Heart
 Liver Antibodies  >337 publications with VEGF165 and Liver
 Kidney Antibodies  >322 publications with VEGF165 and Kidney
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