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VEGF165 recombinant protein

Scan QR to view Datasheet Catalog #    MBS204593
SDS-Page
Unit / Price
0.1 mg  /  $310 +1 FREE 8GB USB
0.5 mg  /  $720 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

VEGF165, Recombinant Protein

★Popular Item★
 Also Known As   

VEGF165, 207-371aa, Human, His tag, E Coli

 Product Synonym Names    Vascular endothelial growth factor A isoform d; VPF; VEGF; VEGF-A
 Product Gene Name   

VEGF165 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MGSSHHHHHH SSGLVPRGSH APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
 OMIM    M32977 mRNA
 3D Structure    ModBase 3D Structure for P15692
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 Host    E Coli
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 Purity/Purification    > 90% by SDS-PAGE
 Form/Format    Liquid. In 20mM Tris-HCl (pH 8.5), 50%glycerol, 5mM DTT, 200mM NaCl, 2mM EDTA
 Concentration    0.5 mg/ml (determined by Bradford assay) (lot specific)
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 Antigen Species    Human
 Tag Information    His-tag
 Endotoxin Level    < 1.0 EU per 1 microgram of protein (determined by LAL method)
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 Preparation and Storage    Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of VEGF165 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for VEGF165 recombinant protein

   Vascular endothelial growth factor (VEGF) is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis. Recombinant human VEGF165 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
 Product Categories/Family for VEGF165 recombinant protein    Hormones & Cytokines
 Applications Tested/Suitable for VEGF165 recombinant protein   

SDS-PAGE

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 SDS-Page of VEGF165 recombinant protein    VEGF165 recombinant protein SDS-Page image
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NCBI/Uniprot data below describe general gene information for VEGF165. It may not necessarily be applicable to this product.
 NCBI GI #    76781483
 NCBI GeneID    7422
 NCBI Accession #    NP_001020539 [Other Products]
 NCBI GenBank Nucleotide #    NM_001025368.2 [Other Products]
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 UniProt Primary Accession #    P15692 [Other Products]
 UniProt Secondary Accession #    O60720; O75875; Q074Z4; Q16889; B5BU86; H0Y2S8; H0Y407; H0Y414; H0Y462; H0Y8N2; H3BLW7 [Other Products]
 UniProt Related Accession #    P15692 [Other Products]
 Molecular Weight    21.3kDa (185aa), conformed by MALDI-TOF
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 NCBI Official Full Name    vascular endothelial growth factor A isoform d
 NCBI Official Synonym Full Names    vascular endothelial growth factor A
 NCBI Official Symbol    VEGFA [Similar Products]
 NCBI Official Synonym Symbols   
VPF; VEGF; MVCD1
[Similar Products]
 NCBI Protein Information    vascular endothelial growth factor A
 UniProt Protein Name    Vascular endothelial growth factor A
 UniProt Synonym Protein Names   
Vascular permeability factor; VPF
 UniProt Gene Name    VEGFA [Similar Products]
 UniProt Synonym Gene Names    VEGF; VEGF-A; VPF [Similar Products]
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 NCBI Summary for VEGF165    This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site. [provided by RefSeq, Nov 2015]
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 UniProt Comments for VEGF165    Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development ().
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Product References and Citations for VEGF165 recombinant protein

   Yasuji ueda., et al. (2004) Cancer Chemother Pharmacol. 54 (1):16-24. ; Neufeld G., et al. (1999) FASEB J. 13 (1):9-22.
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 Research Articles on VEGF165    1. TIPE2 suppressed tumor invasiveness and angiogenesis in non-small cell lung cancer via inhibiting the activation of Rac1 and subsequently weakening its downstream effects, including F-actin polymerization and VEGF expression.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with VEGF165 recombinant proteinPathways associated with VEGF165 recombinant protein
 Reference Product  PubMed Publications
 HIF1A recombinant protein  >783 publications with VEGF165 and HIF1A
 KDR recombinant protein  >493 publications with VEGF165 and KDR
 FLT1 recombinant protein  >242 publications with VEGF165 and FLT1
 TGFB1 recombinant protein  >213 publications with VEGF165 and TGFB1
 HGF recombinant protein  >149 publications with VEGF165 and HGF
 EGF recombinant protein  >136 publications with VEGF165 and EGF
 THBS1 recombinant protein  >71 publications with VEGF165 and THBS1
 IGF1 recombinant protein  >46 publications with VEGF165 and IGF1
 FLT4 recombinant protein  >40 publications with VEGF165 and FLT4
 PIK3CA recombinant protein  >19 publications with VEGF165 and PIK3CA
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Diseases associated with VEGF165 recombinant proteinOrgans/Tissues associated with VEGF165 recombinant protein
 Disease Name  Pubmed Publications
 Neovascularization, Pathologic Antibodies  >2628 publications with VEGF165 and Neovascularization, Pathologic
 Retinal Diseases Antibodies  >2256 publications with VEGF165 and Retinal Diseases
 Adenocarcinoma Antibodies  >896 publications with VEGF165 and Adenocarcinoma
 Inflammation Antibodies  >728 publications with VEGF165 and Inflammation
 Diabetic Retinopathy Antibodies  >699 publications with VEGF165 and Diabetic Retinopathy
 Neoplasm Metastasis Antibodies  >690 publications with VEGF165 and Neoplasm Metastasis
 Diabetes Complications Antibodies  >612 publications with VEGF165 and Diabetes Complications
 Macular Edema Antibodies  >591 publications with VEGF165 and Macular Edema
 Lung Diseases Antibodies  >580 publications with VEGF165 and Lung Diseases
 Heart Diseases Antibodies  >383 publications with VEGF165 and Heart Diseases
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >11035 publications with VEGF165 and Vascular
 Blood Antibodies  >4024 publications with VEGF165 and Blood
 Eye Antibodies  >2178 publications with VEGF165 and Eye
 Lung Antibodies  >743 publications with VEGF165 and Lung
 Bone Antibodies  >734 publications with VEGF165 and Bone
 Muscle Antibodies  >577 publications with VEGF165 and Muscle
 Heart Antibodies  >501 publications with VEGF165 and Heart
 Embryonic Tissue Antibodies  >498 publications with VEGF165 and Embryonic Tissue
 Brain Antibodies  >489 publications with VEGF165 and Brain
 Liver Antibodies  >469 publications with VEGF165 and Liver
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