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VPS24 recombinant protein

Scan QR to view Datasheet Catalog #    MBS204629
SDS-PAGE
Unit / Price
0.1 mg  /  $310 +1 FREE 8GB USB
0.5 mg  /  $720 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

VPS24, Recombinant Protein

 Also Known As   

VPS24, 1-222aa, Human, His tag, E Coli

 Product Synonym Names    Charged multivesicular body protein 3; CGI-149; CHMP3; FLJ38114; NEDF
 Product Gene Name   

VPS24 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MGSSHHHHHH SSGLVPRGSH MGLFGKTQEK PPKELVNEWS LKIRKEMRVV DRQIRDIQRE EEKVKRSVKD AAKKGQKDVC IVLAKEMIRS RKAVSKLYAS KAHMNSVLMG MKNQLAVLRV AGSLQKSTEV MKAMQSLVKI PEIQATMREL SKEMMKAGII EEMLEDTFES MDDQEEMEEE AEMEIDRILF EITAGALGKA PSKVTDALPE PEPPGAMAAS EDEEEEEEAL EAMQSRLATL RS
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 Purity/Purification    > 90% by SDS - PAGE
 Form/Format    Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl,1mM DTT
 Concentration    1.0 mg/ml (determined by Bradford assay) (lot specific)
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 Antigen Species    Human
 Tag    His-tag
 Expression System    E Coli
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 Preparation and Storage    Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of VPS24 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for VPS24 recombinant protein

   VPS24, also known as Charged multivesicular body protein 3(CHMP3), belongs to the vacuolar sorting protein family and function as chromatin modifying proteins. It associates directly with CHMP2 and CHMP4 for the disassembly of ESCRT-III complex in an ATP-dependent manner. During HIV-1 infection, the virus uses the ESCRT-III complex to mediate budding and exocytosis of viral proteins. Overexpression of VPS24 strongly inhibits HIV-1 release. Recombinant human VPS24 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
 Product Categories/Family for VPS24 recombinant protein    Cell Cycle
 Applications Tested/Suitable for VPS24 recombinant protein   

SDS-PAGE

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 SDS-PAGE of VPS24 recombinant protein    VPS24 recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for VPS24. It may not necessarily be applicable to this product.
 NCBI GI #    7706353
 NCBI GeneID    51652
 NCBI Accession #    NP_057163 [Other Products]
 NCBI GenBank Nucleotide #    NM_016079.3 [Other Products]
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 UniProt Secondary Accession #    Q3ZTS9; Q53S71; Q53SU5; Q9NZ51; A8K3W0; B4DG34; B8ZZM0; B8ZZX5 [Other Products]
 UniProt Related Accession #    Q9Y3E7 [Other Products]
 Molecular Weight    27.2 kDa (242aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
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 NCBI Official Full Name    charged multivesicular body protein 3 isoform 1
 NCBI Official Synonym Full Names    charged multivesicular body protein 3
 NCBI Official Symbol    CHMP3 [Similar Products]
 NCBI Official Synonym Symbols   
NEDF; VPS24; CGI-149
[Similar Products]
 NCBI Protein Information    charged multivesicular body protein 3; 25.1 protein; CHMP family, member 3; chromatin-modifying protein 3; neuroendocrine differentiation factor; vacuolar protein sorting 24 homolog; vacuolar protein sorting-associated protein 24
 UniProt Protein Name    Charged multivesicular body protein 3
 UniProt Synonym Protein Names   
Chromatin-modifying protein 3; Neuroendocrine differentiation factor; Vacuolar protein sorting-associated protein 24; hVps24
 Protein Family    Vacuolar protein sorting-associated protein
 UniProt Gene Name    CHMP3 [Similar Products]
 UniProt Synonym Gene Names    CGI149; NEDF; VPS24; hVps24 [Similar Products]
 UniProt Entry Name    CHMP3_HUMAN
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 NCBI Summary for VPS24    This gene encodes a protein that sorts transmembrane proteins into lysosomes/vacuoles via the multivesicular body (MVB) pathway. This protein, along with other soluble coiled-coil containing proteins, forms part of the ESCRT-III protein complex that binds to the endosomal membrane and recruits additional cofactors for protein sorting into the MVB. This protein may also co-immunoprecipitate with a member of the IFG-binding protein superfamily. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ring finger protein 103 (RNF103) gene. [provided by RefSeq, Nov 2010]
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 UniProt Comments for VPS24    VPS24: Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Selectively binds to phosphatidylinositol 3,5-bisphosphate PtdIns(3,5)P2 and PtdIns(3,4)P2 in preference to other phosphoinositides tested. Involved in late stages of cytokinesis. Plays a role in endosomal sorting/trafficking of EGF receptor. Isoform 2 prevents stress- mediated cell death and accumulation of reactive oxygen species when expressed in yeast cells. Belongs to the SNF7 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 2p11.2

Cellular Component: late endosome membrane; cytoplasmic membrane-bound vesicle; plasma membrane; cytosol

Molecular Function: protein binding; protein homodimerization activity; phosphatidylcholine binding

Biological Process: protein polymerization; protein transport; cell separation during cytokinesis; viral reproduction; apoptosis; protein heterooligomerization; vacuolar transport; regulation of viral reproduction; viral infectious cycle; endosome transport; mitotic metaphase plate congression; nuclear organization and biogenesis
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Product References and Citations for VPS24 recombinant protein

   Muziol T. et al. (2006) Dev. Cell. 10: 821-830.
Bache K.G. et al. (2006) Mol. Biol. Cell 17: 2513-2523.
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 Research Articles on VPS24    1. Protein kinase CK2 alpha is involved in the phosphorylation of the ESCRT-III subunits CHMP3 and CHMP2B, as well as of VPS4B/SKD1, an ATPase that mediates ESCRT-III disassembly.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with VPS24 recombinant proteinPathways associated with VPS24 recombinant protein
 Reference Product  PubMed Publications
 CHMP2A recombinant protein  >9 publications with VPS24 and CHMP2A
 STAMBP recombinant protein  >6 publications with VPS24 and STAMBP
 CHMP4B recombinant protein  >5 publications with VPS24 and CHMP4B
 CHMP4A recombinant protein  >5 publications with VPS24 and CHMP4A
 CHMP6 recombinant protein  >4 publications with VPS24 and CHMP6
 VPS4A recombinant protein  >3 publications with VPS24 and VPS4A
 SNF8 recombinant protein  >2 publications with VPS24 and SNF8
 CHMP5 recombinant protein  >2 publications with VPS24 and CHMP5
 VTA1 recombinant protein  >1 publications with VPS24 and VTA1
 Products by Pathway  Pathway Diagram
 Budding And Maturation Of HIV Virion Pathway antibodies  Budding And Maturation Of HIV Virion Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 ESCRT-III Complex Pathway antibodies  ESCRT-III Complex Pathway Diagram
 ESCRT-III Complex Pathway antibodies  ESCRT-III Complex Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Endosomal Sorting Complex Required For Transport (ESCRT) Pathway antibodies  Endosomal Sorting Complex Required For Transport (ESCRT) Pathway Diagram
 HIV Infection Pathway antibodies  HIV Infection Pathway Diagram
 HIV Life Cycle Pathway antibodies  HIV Life Cycle Pathway Diagram
 Internalization Of ErbB1 Pathway antibodies  Internalization Of ErbB1 Pathway Diagram
Organs/Tissues associated with VPS24 recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >11 publications with VPS24 and Nerve
 Prostate Antibodies  >3 publications with VPS24 and Prostate
 Kidney Antibodies  >3 publications with VPS24 and Kidney
 Lung Antibodies  >3 publications with VPS24 and Lung
 Heart Antibodies  >1 publications with VPS24 and Heart
 Liver Antibodies  >1 publications with VPS24 and Liver
 Connective Tissue Antibodies  >1 publications with VPS24 and Connective Tissue
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