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VEGF recombinant protein :: Vascular Endothelial Growth Factor 164AA Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS553124 VEGF recombinant protein
Unit / Price
0.002 mg  /  $145 +1 FREE 8GB USB
0.01 mg  /  $220 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Vascular Endothelial Growth Factor 164AA (VEGF), Recombinant Protein

 Also Known As   

Mouse Vascular Endothelial Growth Factor 164AA Recombinant

 Product Synonym Names    vegf; vascular endothelial
 Product Gene Name   

VEGF recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MNFLLSWVHW TLALLLYLHH AKWSQAAPTT EGEQKSHEVI KFMDVYQRSY CRPIETLVDI FQEYPDEIEY IFKPSCVPLM RCAGCCNDEA LECVPTSESN ITMQIMRIKP HQSQHIGEMS FLQHSRCECR PKKDRTKPEN HCEPCSERRK HLFVQDPQTC KCSCKNTDSR CKARQLELNE RTCRCDKPRR
 3D Structure    ModBase 3D Structure for Q00731
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 Host    E Coli
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 Purity/Purification    >95%, as determined by SDS-PAGE and HPLC
 Form/Format    Recombinant mouse VEGF was lyophilized from 0.2 um filtered PBS solution, pH 7.0.
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 Domain    cystine knot growth factor
 Host Note    Optimized DNA sequence encoding Mouse Vascular Endothelial Growth Factor mature chain was expressed in Escherichia Coli.
 Endotoxin    Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
 Reconstitution    A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
 Molecular Weight Note    Native mouse VEGF-164 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 14 kDa. Recombinant Vascular endothelial growth factor is a disulfide-linked homodimer protein consisting of 165 amino acid residue subunits, and migrates as an approximately 28 kDa protein under non-reducing and as 14kDa under reducing conditions in SDS-PAGE.
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 Preparation and Storage    The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.
 Other Notes    Small volumes of VEGF recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for VEGF recombinant protein

   VEGF is a homodimeric heavily glycosylated protein.The human factor occurs in several molecular variants of 121, 162, 145, 148, 165, 183, 189, 206 amino acids, arising by alternative splicing of the mRNA.The splice forms of VEGF differ in biological properties such as the receptor types, which they recognize and their interaction with heparan sulfate proteoglycans. The 165 amino acid form of the factor is the most common form in most tissues. Kaposi sarcomas express VEGF121 and VEGF165. VEGF121 and VEGF165 are soluble secreted forms of the factor while VEGF189 and VEGF206 are mostly bound to heparin-containing proteoglycans in the cell surface or in the basement membrane. A high-affinity glycoprotein receptor of 170-235 kDa is expressed on vascular endothelial cells. The interaction of VEGF with heparin-like molecules of the extracellular matrix is required for efficient receptor binding. Protamine sulfate and suramin are capable of replacing the receptor-bound factor. The high-affinity receptor for VEGF, now known as VEGFR1, has been identified as the gene product of the FLT-1. Another receptor for VEGF, now known as VEGFR2, is KDR, also known as FLK-1. A factor that competes with the 165 amino acid form of VEGF for receptor binding is PLGF. A third receptor type, VEGFR3 is known also as FLT- 4. An isoform-specific receptor for VEGF165 has been identified as human Neuropilin-1.
 Product Categories/Family for VEGF recombinant protein    Cytokines
NCBI/Uniprot data below describe general gene information for VEGF. It may not necessarily be applicable to this product.
 NCBI GI #    160358799
 NCBI GeneID    22339
 NCBI Accession #    NP_001020421.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_001025250.3 [Other Products]
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 UniProt Primary Accession #    Q00731 [Other Products]
 UniProt Secondary Accession #    O70123; Q66S31; Q6GT23; Q6WZL9 [Other Products]
 UniProt Related Accession #    Q00731; Q5UD54; Q6YLN3; Q80UA0; A0FKR4 [Other Products]
 Molecular Weight    25,283 Da
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 NCBI Official Full Name    vascular endothelial growth factor A isoform 1
 UniProt Protein Name    Vascular endothelial growth factor A
 UniProt Synonym Protein Names   
Vascular permeability factor
 UniProt Gene Name    Vegfa [Similar Products]
 UniProt Synonym Gene Names    Vegf; VEGF-A; VPF [Similar Products]
 UniProt Entry Name    VEGFA_MOUSE
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 NCBI Summary for VEGF    This gene product is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Also, alternative translation initiation from non-AUG (CUG) and AUG start sites in some transcript variants, give rise to additional isoforms. The expression of some isoforms derived from AUG start codon is affected by a small upstream open reading frame, which is located within an internal ribosome entry site. [provided by RefSeq, Nov 2013]
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 UniProt Comments for VEGF    VEGF: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the PDGF/VEGF growth factor family. 13 isoforms of the human protein are produced by alternative promoter.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; Cytokine

Cellular Component: extracellular space; cell surface; membrane; cytoplasm; plasma membrane; extracellular region; basement membrane; secretory granule

Molecular Function: heparin binding; identical protein binding; protein homodimerization activity; growth factor activity; extracellular matrix binding; cytokine activity; platelet-derived growth factor receptor binding; vascular endothelial growth factor receptor 1 binding; receptor agonist activity; vascular endothelial growth factor receptor binding; protein binding; vascular endothelial growth factor receptor 2 binding; protein heterodimerization activity; fibronectin binding; receptor binding; chemoattractant activity

Biological Process: heart morphogenesis; positive regulation of positive chemotaxis; macrophage differentiation; positive regulation of cell adhesion; multicellular organismal development; positive regulation of receptor internalization; cell maturation; basophil chemotaxis; regulation of cell shape; positive regulation of MAP kinase activity; positive chemotaxis; positive regulation of mesenchymal cell proliferation; mesoderm development; negative regulation of neuron apoptosis; kidney development; positive regulation of neuroblast proliferation; nervous system development; T-helper 1 type immune response; positive regulation of signal transduction; monocyte differentiation; mRNA stabilization; positive regulation of blood vessel endothelial cell migration; activation of CREB transcription factor; positive regulation of protein amino acid autophosphorylation; regulation of endothelial cell differentiation; positive regulation of vascular permeability; regulation of transcription from RNA polymerase II promoter; patterning of blood vessels; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of angiogenesis; eye photoreceptor cell development; camera-type eye morphogenesis; branching morphogenesis of a tube; cell migration during sprouting angiogenesis; cardiac muscle fiber development; positive regulation of cell division; activation of protein kinase activity; positive regulation of axon extension involved in axon guidance; blood vessel morphogenesis; endothelial cell migration; neuron development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; regulation of cGMP metabolic process; alveolus development; surfactant homeostasis; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; lactation; post-embryonic camera-type eye development; positive regulation of smooth muscle cell proliferation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of caspase activity; positive regulation of vascular endothelial growth factor receptor signaling pathway; induction of positive chemotaxis; positive regulation of focal adhesion formation; epithelial cell differentiation; vasculature development; ovarian follicle development; positive regulation of cell proliferation; lymphangiogenesis; negative regulation of programmed cell death; angiogenesis; cell differentiation; negative regulation of bone resorption; blood vessel development; cell migration; in utero embryonic development; lumen formation; positive regulation of cell motility; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein kinase B signaling cascade; cell proliferation; positive regulation of protein complex assembly; response to hypoxia; artery morphogenesis; blood vessel remodeling; negative regulation of cell-cell adhesion; sprouting angiogenesis; positive regulation of protein amino acid phosphorylation; vascular endothelial growth factor receptor signaling pathway; positive regulation of cell migration; growth; lung development
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Product References and Citations for VEGF recombinant protein

   decreased nephrin and glepp-1, but increased VEGF, flt-1, and nitrotyrosine, expressions in kidney tissue sections from women with preeclampsia .Reproductive Sciences, Oct 2009; 16: 970 - 979.
significantly higher levels of vascular endothelial growth factor (VEGF) and shorter survival times for patients with primary operable triple-negative breast cancer .Ann. Onc., Oct 2009; 20: 1639 - 1646.
comparative analysis of metastasis variants derived from human prostate carcinoma cells: roles in intravasation of VEGF-mediated angiogenesis and upa-mediated invasion .Am. J. Pathol., Oct 2009; 175: 1638 - 1652.
autocrine loop between vascular endothelial growth factor (VEGF) -c and VEGF receptor-3 positively regulates tumor-associated lymphangiogenesis in oral squamoid cancer cells .Am. J. Pathol., Oct 2009; 175: 1709 - 1721.
polyneuropathy, organomegaly, endocrinopathy, monoclonal protein, skin changes (poems) syndrome refractory to complete serum vascular endothelial growth factor (VEGF) blockade: insights from sequential VEGF monitoring .NDT Plus, Oct 2009; 2: 357 - 359.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with VEGF recombinant proteinPathways associated with VEGF recombinant protein
 Reference Product  PubMed Publications
 Nfatc4 recombinant protein  >1 publications with VEGF and Nfatc4
 Products by Pathway  Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Formation Of Platelet Plug Pathway antibodies  Formation Of Platelet Plug Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
Diseases associated with VEGF recombinant proteinOrgans/Tissues associated with VEGF recombinant protein
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1622 publications with VEGF and Neoplasms
 Neovascularization, Pathologic Antibodies  >1079 publications with VEGF and Neovascularization, Pathologic
 Cardiovascular Diseases Antibodies  >616 publications with VEGF and Cardiovascular Diseases
 Macular Degeneration Antibodies  >386 publications with VEGF and Macular Degeneration
 Retinal Degeneration Antibodies  >373 publications with VEGF and Retinal Degeneration
 Adenocarcinoma Antibodies  >358 publications with VEGF and Adenocarcinoma
 Neoplasm Metastasis Antibodies  >275 publications with VEGF and Neoplasm Metastasis
 Inflammation Antibodies  >214 publications with VEGF and Inflammation
 Edema Antibodies  >203 publications with VEGF and Edema
 Lung Diseases Antibodies  >202 publications with VEGF and Lung Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1704 publications with VEGF and Blood
 Eye Antibodies  >612 publications with VEGF and Eye
 Muscle Antibodies  >294 publications with VEGF and Muscle
 Embryonic Tissue Antibodies  >276 publications with VEGF and Embryonic Tissue
 Lung Antibodies  >273 publications with VEGF and Lung
 Heart Antibodies  >215 publications with VEGF and Heart
 Kidney Antibodies  >182 publications with VEGF and Kidney
 Skin Antibodies  >172 publications with VEGF and Skin
 Liver Antibodies  >170 publications with VEGF and Liver
 Brain Antibodies  >159 publications with VEGF and Brain
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