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WHSC2 recombinant protein

Scan QR to view Datasheet Catalog #    MBS203792
SDS-Page
Unit / Price
0.05 mg  /  $375 +1 FREE 8GB USB
0.25 mg  /  $925 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

WHSC2, Recombinant Protein

★Popular Item★
 Also Known As   

WHSC2, 1-539aa, Human, His tag, E Coli

 Product Synonym Names    Wolf-Hirschhorn syndrome candidate 2; NELF-A; NELFA; P/OKcl.15; Negative elongation factor A
 Product Gene Name   

WHSC2 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MGSSHHHHHH SSGLVPRGSH MPGQRRALSP KMASMRESDT GLWLHNKLGA TDELWAPPSI ASLLTAAVID NIRLCFHGLS SAVKLKLLLG TLHLPRRTVD EMKGALMEII QLASLDSDPW VLMVADILKS FPDTGSLNLE LEEQNPNVQD ILGELREKVG ECEASAMLPL ECQYLNKNAL TTLAGPLTPP VKHFQLKRKP KSATLRAELL QKSTETAQQL KRSAGVPFHA KGRGLLRKMD TTTPLKGIPK QAPFRSPTAP SVFSPTGNRT PIPPSRTLLR KERGVKLLDI SELDMVGAGR EAKRRRKTLD AEVVEKPAKE ETVVENATPD YAAGLVSTQK LGSLNNEPAL PSTSYLPSTP SVVPASSYIP SSETPPAPSS REASRPPEEP SAPSPTLPAQ FKQRAPMYNS GLSPATPTPA APTSPLTPTT PPAVAPTTQT PPVAMVAPQT QAPAQQQPKK NLSLTREQMF AAQEMFKTAN KVTRPEKALI LGFMAGSREN PCQEQGDVIQ IKLSEHTEDL PKADGQGSTT MLVDTVFEMN YATGQWTRFK KYKPMTNVS
 OMIM    AF101434 mRNA
 3D Structure    ModBase 3D Structure for Q9H3P2
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 Host    E Coli
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 Purity/Purification    > 85% by SDS-PAGE
 Form/Format    Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 1mM EDTA, 20% glycerol
 Concentration    0.25 mg/ml (determined by Bradford assay) (lot specific)
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 Antigen Species    Human
 Tag Information    His-tag
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 Preparation and Storage    Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of WHSC2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for WHSC2 recombinant protein

   WHSC2, also known as NELF-A, is a protein factor required for DRB-sensitive transcription. This protein is one of the five components of the multisubunit NELF complex that cooperates with DSIF to repress RNA polymerase II elongation. Wolf-Hirschhorn syndrome is a multiple malformation syndrome characterized by mental and developmental defects resulting from a hemizygous deletion of the distal short arm of chromosome 4 (4p16.3). Recombinant human WHSC2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
 Product Categories/Family for WHSC2 recombinant protein    Transcription & Translation
 Applications Tested/Suitable for WHSC2 recombinant protein   

SDS-PAGE

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 SDS-Page of WHSC2 recombinant protein    WHSC2 recombinant protein SDS-Page image
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NCBI/Uniprot data below describe general gene information for WHSC2. It may not necessarily be applicable to this product.
 NCBI GI #    194733742
 NCBI GeneID    7469
 NCBI Accession #    NP_005654 [Other Products]
 NCBI GenBank Nucleotide #    NM_005663.4 [Other Products]
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 UniProt Primary Accession #    Q9H3P2 [Other Products]
 UniProt Secondary Accession #    O95392; A2A2T1 [Other Products]
 UniProt Related Accession #    Q9H3P2 [Other Products]
 Molecular Weight    60.6 kDa (559aa)
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 NCBI Official Full Name    negative elongation factor A
 NCBI Official Synonym Full Names    negative elongation factor complex member A
 NCBI Official Symbol    NELFA [Similar Products]
 NCBI Official Synonym Symbols   
WHSC2; NELF-A; P/OKcl.15
[Similar Products]
 NCBI Protein Information    negative elongation factor A
 UniProt Protein Name    Negative elongation factor A
 UniProt Synonym Protein Names   
Wolf-Hirschhorn syndrome candidate 2 protein
 UniProt Gene Name    NELFA [Similar Products]
 UniProt Synonym Gene Names    WHSC2; NELF-A [Similar Products]
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 NCBI Summary for WHSC2    This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation. [provided by RefSeq, Jul 2008]
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 UniProt Comments for WHSC2    Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. In vitro, the NELFA:NELFCD subcomplex binds to ssDNA and ssRNA in a sequence- and structure-dependent manner. Probably required to interact with the RNA polymerase II complex.
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Product References and Citations for WHSC2 recombinant protein

   Care A., et al. (2007) Nat Med. 13 (5):613-8.; Mariotti M., et al. (2000) FEBS Lett. 487 (2):166-70.;
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 Research Articles on WHSC2    1. A positively charged face of NELF-AC is involved in RNA binding, whereas the opposite face of the NELF-AC subcomplex binds NELF-B. NELF-B is predicted to form a HEAT repeat fold, also binds RNA in vivo, and anchors the subunit NELF-E, which is confirmed to bind RNA in vivo.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with WHSC2 recombinant proteinPathways associated with WHSC2 recombinant protein
 Reference Product  PubMed Publications
 WHSC2 recombinant protein  >1 publications with WHSC2 and WHSC2
 Products by Pathway  Pathway Diagram
 Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway antibodies  Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway antibodies  Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway Diagram
 Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway antibodies  Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway Diagram
 Formation Of RNA Pol II Elongation Complex Pathway antibodies  Formation Of RNA Pol II Elongation Complex Pathway Diagram
 Formation Of The Early Elongation Complex Pathway antibodies  Formation Of The Early Elongation Complex Pathway Diagram
 Formation Of The HIV-1 Early Elongation Complex Pathway antibodies  Formation Of The HIV-1 Early Elongation Complex Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 HIV Infection Pathway antibodies  HIV Infection Pathway Diagram
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