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aFGF recombinant protein :: acidic Fibroblast Growth Factor Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS553115 aFGF recombinant protein
Unit / Price
0.01 mg  /  $145 +1 FREE 8GB USB
0.05 mg  /  $220 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

acidic Fibroblast Growth Factor (aFGF), Recombinant Protein

 Also Known As   

Mouse acidic Fibroblast Growth Factor Recombinant

 Product Synonym Names    HBGF-1; afgf; Beta-endothelial cell growth factor
 Product Gene Name   

aFGF recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MAEGEITTFA ALTERFNLPL GNYKKPKLLY CSNGGHFLRI LPDGTVDGTR DRSDQHIQLQ LSAESAGEVY IKGTETGQYL AMDTEGLLYG SQTPNEECLF LERLEENHYN TYTSKKHAEK NWFVGLKKNG SCKRGPRTHY GQKAILFLPL PVSSD
 3D Structure    ModBase 3D Structure for P61148
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 Host    E Coli
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 Purity/Purification    >95%, as determined by SDS-PAGE and HPLC
 Form/Format    Recombinant mouse aFGF was lyophilized from a 0.2 um filtered PBS solution.
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 Domain    HBGF/IL1
 Host Note    Optimized DNA sequence encoding Mouse acidic FGF mature chain was expressed in Escherichia Coli.
 Endotoxin    Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
 Reconstitution    A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
 Molecular Weight Note    Native Mouse aFGF, generated by the proteolytic removal of the signal peptide
and propeptide, the moleculte has a calculated mass of 16 kDa.

Recombinant FGF acidic is a disulfide-linked monomer protein consisting of 141 amino acids and migrates as an approximately 16 kDa protein under reducing and non-reducing conditions.
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 Preparation and Storage    The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.
 Other Notes    Small volumes of aFGF recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for aFGF recombinant protein

   aFGF and bFGF display 55 % homology which is limited, however, to a few functional domains. The structure of aFGF, revealed by X-ray crystallography, shows a similar folding structure as IL1. Because aFGF binds to the same receptor as bFGF, aFGF displays more or less the same spectrum of activities. It is, however, generally less active than bFGF in similar assays. FGF receptors are encoded by a gene family consisting of at least four receptor tyrosine kinases that transduce signals important in a variety of developmental and physiological processes related to cell growth and differentiation.
 Product Categories/Family for aFGF recombinant protein    Cytokines
NCBI/Uniprot data below describe general gene information for aFGF. It may not necessarily be applicable to this product.
 NCBI GI #    6753850
 NCBI GeneID    14164
 NCBI Accession #    NP_034327.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_010197.3 [Other Products]
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 UniProt Primary Accession #    P61148 [Other Products]
 UniProt Secondary Accession #    P10935 [Other Products]
 UniProt Related Accession #    O54837; P61148; Q6ZWS1; Q71UP6 [Other Products]
 Molecular Weight    17,418 Da
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 NCBI Official Full Name    fibroblast growth factor 1
 UniProt Protein Name    Fibroblast growth factor 1
 UniProt Synonym Protein Names   
Acidic fibroblast growth factor; aFGF; Heparin-binding growth factor 1
 Protein Family    Acidic fibroblast growth factor intracellular-binding protein
 UniProt Gene Name    Fgf1 [Similar Products]
 UniProt Synonym Gene Names    Fgf-1; Fgfa; FGF-1; aFGF; HBGF-1 [Similar Products]
 UniProt Entry Name    FGF1_MOUSE
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 UniProt Comments for aFGF    FGF1: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP1. Part of a Cu(2+)-dependent multiprotein aggregate containing FGF1, S100A13 and SYT1. Interacts with SYT1. Interacts with S100A13. Belongs to the heparin-binding growth factors family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Motility/polarity/chemotaxis; Cell development/differentiation

Cellular Component: nucleoplasm; proteinaceous extracellular matrix; extracellular space; cytoplasm; nucleolus; extracellular region; cytosol; nucleus

Molecular Function: heparin binding; protein binding; growth factor activity; Hsp70 protein binding; fibroblast growth factor receptor binding; receptor binding

Biological Process: fibroblast growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; multicellular organismal development; positive regulation of cholesterol biosynthetic process; cardiac muscle cell proliferation; positive regulation of MAP kinase activity; cell proliferation; positive regulation of angiogenesis; induction of an organ; positive regulation of cell division; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; angiogenesis; cell differentiation; positive regulation of epithelial cell proliferation; positive regulation of cell migration; lung development
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Product References and Citations for aFGF recombinant protein

   expression of acidic fibroblast growth factor (aFGF) and fibroblast growth factor receptor 4 (fgfr4) in breast fibroadenomas .J. Clin. Pathol., Jan 2001; 54: 37 - 41.
requirement of phosphatidylinositol 3-kinase activity for translocation of exogenous aFGF to the cytosol and nucleus .J. Biol. Chem., Apr 2000; 275: 11972.
angiogenic potential of perivascularly delivered aFGF in a porcine model of chronic myocardial ischemia .Am J Physiol Heart Circ Physiol, Mar 1998; 274: H930 - H936.
the fibroblast growth factor receptor-1 is necessary for the induction of neurite outgrowth in pc12 cells by aFGF .J. Neurosci., Aug 1996; 16: 4579.
the major acidic fibroblast growth factor (aFGF) -stimulated phosphoprotein from bovine liver plasma membranes has aFGF-stimulated kinase, autoadenylylation, and alkaline nucleotide phosphodiesterase activities .J. Biol. Chem., Dec 1993; 268: 27318 - 27326.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with aFGF recombinant proteinDiseases associated with aFGF recombinant protein
 Products by Pathway  Pathway Diagram
 Downstream Signaling Of Activated FGFR Pathway antibodies  Downstream Signaling Of Activated FGFR Pathway Diagram
 ESC Pluripotency Pathways antibodies  ESC Pluripotency Pathways Diagram
 FGFR Ligand Binding And Activation Pathway antibodies  FGFR Ligand Binding And Activation Pathway Diagram
 FGFR1 Ligand Binding And Activation Pathway antibodies  FGFR1 Ligand Binding And Activation Pathway Diagram
 FGFR1b Ligand Binding And Activation Pathway antibodies  FGFR1b Ligand Binding And Activation Pathway Diagram
 FGFR1c Ligand Binding And Activation Pathway antibodies  FGFR1c Ligand Binding And Activation Pathway Diagram
 FGFR2 Ligand Binding And Activation Pathway antibodies  FGFR2 Ligand Binding And Activation Pathway Diagram
 FGFR2b Ligand Binding And Activation Pathway antibodies  FGFR2b Ligand Binding And Activation Pathway Diagram
 FGFR2c Ligand Binding And Activation Pathway antibodies  FGFR2c Ligand Binding And Activation Pathway Diagram
 FGFR3 Ligand Binding And Activation Pathway antibodies  FGFR3 Ligand Binding And Activation Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >330 publications with aFGF and Neoplasms
 Cardiovascular Diseases Antibodies  >116 publications with aFGF and Cardiovascular Diseases
 Neovascularization, Pathologic Antibodies  >85 publications with aFGF and Neovascularization, Pathologic
 Ischemia Antibodies  >83 publications with aFGF and Ischemia
 Breast Neoplasms Antibodies  >58 publications with aFGF and Breast Neoplasms
 Inflammation Antibodies  >43 publications with aFGF and Inflammation
 Adenocarcinoma Antibodies  >39 publications with aFGF and Adenocarcinoma
 Urologic Diseases Antibodies  >39 publications with aFGF and Urologic Diseases
 Genital Diseases, Male Antibodies  >33 publications with aFGF and Genital Diseases, Male
 Prostatic Diseases Antibodies  >32 publications with aFGF and Prostatic Diseases
Organs/Tissues associated with aFGF recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >266 publications with aFGF and Brain
 Muscle Antibodies  >227 publications with aFGF and Muscle
 Embryonic Tissue Antibodies  >159 publications with aFGF and Embryonic Tissue
 Lung Antibodies  >104 publications with aFGF and Lung
 Liver Antibodies  >104 publications with aFGF and Liver
 Heart Antibodies  >102 publications with aFGF and Heart
 Eye Antibodies  >102 publications with aFGF and Eye
 Kidney Antibodies  >77 publications with aFGF and Kidney
 Skin Antibodies  >75 publications with aFGF and Skin
 Spinal Cord Antibodies  >64 publications with aFGF and Spinal Cord
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