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| Product Name
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acidic Fibroblast Growth Factor (aFGF), Recombinant Protein ★Popular Item★
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| Also Known As
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Mouse acidic Fibroblast Growth Factor Recombinant
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| Product Synonym Names
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HBGF-1; afgf; Beta-endothelial cell growth factor
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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| Sequence
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MAEGEITTFA ALTERFNLPL GNYKKPKLLY CSNGGHFLRI LPDGTVDGTR DRSDQHIQLQ LSAESAGEVY IKGTETGQYL AMDTEGLLYG SQTPNEECLF LERLEENHYN TYTSKKHAEK NWFVGLKKNG SCKRGPRTHY GQKAILFLPL PVSSD
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| 3D Structure
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ModBase 3D Structure for P61148
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| Host
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E Coli
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| Purity/Purification
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>95%, as determined by SDS-PAGE and HPLC
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| Form/Format
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Recombinant mouse aFGF was lyophilized from a 0.2 um filtered PBS solution.
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| Domain
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HBGF/IL1
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| Host Note
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Optimized DNA sequence encoding Mouse acidic FGF mature chain was expressed in Escherichia Coli.
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| Endotoxin
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Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
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| Reconstitution
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A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
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| Molecular Weight Note
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Native Mouse aFGF, generated by the proteolytic removal of the signal peptide
and propeptide, the moleculte has a calculated mass of 16 kDa.
Recombinant FGF acidic is a disulfide-linked monomer protein consisting of 141 amino acids and migrates as an approximately 16 kDa protein under reducing and non-reducing conditions.
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| Preparation and Storage
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The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
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| Other Notes
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Small volumes of aFGF recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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aFGF and bFGF display 55 % homology which is limited, however, to a few functional domains. The structure of aFGF, revealed by X-ray crystallography, shows a similar folding structure as IL1. Because aFGF binds to the same receptor as bFGF, aFGF displays more or less the same spectrum of activities. It is, however, generally less active than bFGF in similar assays. FGF receptors are encoded by a gene family consisting of at least four receptor tyrosine kinases that transduce signals important in a variety of developmental and physiological processes related to cell growth and differentiation.
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| Product Categories/Family for aFGF recombinant protein
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Cytokines
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| NCBI/Uniprot data below describe general gene information for aFGF. It may not necessarily be applicable to this product. |
| NCBI GI #
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6753850
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| NCBI GeneID
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14164
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| NCBI Accession #
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NP_034327.1
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_010197.3
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[Other Products]
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| UniProt Primary Accession #
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P61148
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[Other Products]
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| UniProt Secondary Accession #
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P10935
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[Other Products]
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| UniProt Related Accession #
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O54837; P61148; Q6ZWS1; Q71UP6
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[Other Products]
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| Molecular Weight
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17,418 Da
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TOP
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| NCBI Official Full Name
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fibroblast growth factor 1
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| UniProt Protein Name
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Fibroblast growth factor 1
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| UniProt Synonym Protein Names
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Acidic fibroblast growth factor; aFGF; Heparin-binding growth factor 1
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| Protein Family
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Acidic fibroblast growth factor intracellular-binding protein
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| UniProt Gene Name
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Fgf1
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[Similar Products]
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| UniProt Synonym Gene Names
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Fgf-1; Fgfa; FGF-1; aFGF; HBGF-1
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[Similar Products]
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| UniProt Entry Name
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FGF1_MOUSE
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| UniProt Comments for aFGF
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FGF1: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP1. Part of a Cu(2+)-dependent multiprotein aggregate containing FGF1, S100A13 and SYT1. Interacts with SYT1. Interacts with S100A13. Belongs to the heparin-binding growth factors family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cytokine; Motility/polarity/chemotaxis; Cell development/differentiation
Cellular Component: nucleoplasm; proteinaceous extracellular matrix; extracellular space; cytoplasm; nucleolus; extracellular region; cytosol; nucleus
Molecular Function: heparin binding; protein binding; growth factor activity; Hsp70 protein binding; fibroblast growth factor receptor binding; receptor binding
Biological Process: fibroblast growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; multicellular organismal development; positive regulation of cholesterol biosynthetic process; cardiac muscle cell proliferation; positive regulation of MAP kinase activity; cell proliferation; positive regulation of angiogenesis; induction of an organ; positive regulation of cell division; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; angiogenesis; cell differentiation; positive regulation of epithelial cell proliferation; positive regulation of cell migration; lung development
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• expression of acidic fibroblast growth factor (aFGF) and fibroblast growth factor receptor 4 (fgfr4) in breast fibroadenomas .J. Clin. Pathol., Jan 2001; 54: 37 - 41.
• requirement of phosphatidylinositol 3-kinase activity for translocation of exogenous aFGF to the cytosol and nucleus .J. Biol. Chem., Apr 2000; 275: 11972.
• angiogenic potential of perivascularly delivered aFGF in a porcine model of chronic myocardial ischemia .Am J Physiol Heart Circ Physiol, Mar 1998; 274: H930 - H936.
• the fibroblast growth factor receptor-1 is necessary for the induction of neurite outgrowth in pc12 cells by aFGF .J. Neurosci., Aug 1996; 16: 4579.
• the major acidic fibroblast growth factor (aFGF) -stimulated phosphoprotein from bovine liver plasma membranes has aFGF-stimulated kinase, autoadenylylation, and alkaline nucleotide phosphodiesterase activities .J. Biol. Chem., Dec 1993; 268: 27318 - 27326.
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Pathways associated with aFGF recombinant protein | Diseases associated with aFGF recombinant protein |
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| Organs/Tissues associated with aFGF recombinant protein |
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Recombinant Protein 
