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bFGF recombinant protein :: basic Fibroblast Growth Factor Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS553111 bFGF recombinant protein
Unit / Price
0.01 mg  /  $145 +1 FREE 8GB USB
0.05 mg  /  $220 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

basic Fibroblast Growth Factor (bFGF), Recombinant Protein

 Also Known As   

Mouse basic Fibroblast Growth Factor Recombinant

 Product Synonym Names    bfgf; fgf basic; Fibroblast Growth Factor
 Product Gene Name   

bFGF recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MAASGITSLP ALPEDGGAAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHVK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTEEC FFFERLESNN YNTYRSRKYS SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS
 3D Structure    ModBase 3D Structure for P15655
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 Host    E Coli
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 Purity/Purification    >98%, as determined by SDS-PAGE and HPLC
 Form/Format    Recombinant mouse basic FGF was lyophilized from 0.2 um filtered PBS solution, pH 7.0.
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 Domain    HBGF/IL1
 Host Note    Optimized DNA sequence encoding Mouse basic Fibroblast Growth Factor mature chain was expressed in Escherichia Coli.
 Endotoxin    Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
 Reconstitution    A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
 Molecular Weight Note    Native Mouse FGF basic is generated by the proteolytic removal of the signal peptide
and propeptide,the molecule has a calulated mass of 16 kDa.

Recombinant mouse bFGF is a disulfide-linked homodimeric protein consisting of 146 amino acid residue subunits, and migrates as an approximately 16 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
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 Preparation and Storage    The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.
 Other Notes    Small volumes of bFGF recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for bFGF recombinant protein

   basic Fibroblast Growth Factor is found in almost all tissues of mesodermal and neuroectodermal origin and also in tumors derived from these tissues. Endothelial cells produce large amounts of this factor. Some bFGF is associated with the extracellular matrix of the subendothelial cells. Many cells express bFGF only transiently and store it in a biologically inactive form. The mechanism by which the factor is released by the cells is not known. It is released after tissue injuries and during inflammatory processes. FGF receptors are encoded by a gene family consisting of at least four receptor tyrosine kinases that transduce signals important in a variety of developmental and physiological processes related to cell growth and differentiation. bFGF stimulates the growth of fibroblasts, myoblasts, osteoblasts, neuronal cells, endothelial cells, keratinocytes, chondrocytes, and many other cell types.
 Product Categories/Family for bFGF recombinant protein    Cytokines
NCBI/Uniprot data below describe general gene information for bFGF. It may not necessarily be applicable to this product.
 NCBI GI #    7106315
 NCBI GeneID    14173
 NCBI Accession #    NP_032032.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_008006.2 [Other Products]
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 UniProt Primary Accession #    P15655 [Other Products]
 UniProt Related Accession #    P15655; Q541T2; Q5SUD8; Q7TPG9; Q7TPH0; Q925A1; Q925A2; Q925A3 [Other Products]
 Molecular Weight    17,153 Da
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 NCBI Official Full Name    fibroblast growth factor 2
 UniProt Protein Name    Fibroblast growth factor 2
 UniProt Synonym Protein Names   
Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2
 UniProt Gene Name    Fgf2 [Similar Products]
 UniProt Synonym Gene Names    Fgf-2; FGF-2; bFGF; HBGF-2 [Similar Products]
 UniProt Entry Name    FGF2_MOUSE
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 UniProt Comments for bFGF    FGF2: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2. Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue. Belongs to the heparin-binding growth factors family. 4 isoforms of the human protein are produced by alternative initiation.

Protein type: Activator; Motility/polarity/chemotaxis

Cellular Component: extracellular space; cytoplasm; extracellular region; nucleus; cytosol

Molecular Function: heparin binding; protein binding; growth factor activity; ligand-dependent nuclear receptor transcription coactivator activity; cytokine activity; chemoattractant activity; receptor binding; fibroblast growth factor receptor binding

Biological Process: wound healing; activation of MAPKK activity; regulation of cell cycle; multicellular organismal development; positive regulation of smooth muscle cell proliferation; positive regulation of transcription, DNA-dependent; adrenocorticotropin hormone secreting cell differentiation; thyroid stimulating hormone secreting cell differentiation; cardiac muscle cell proliferation; hyaluronan catabolic process; growth factor dependent regulation of satellite cell proliferation; positive regulation of MAP kinase activity; negative regulation of cell proliferation; embryonic development ending in birth or egg hatching; substantia nigra development; glial cell differentiation; positive chemotaxis; induction of an organ; positive regulation of cell proliferation; regulation of retinal cell programmed cell death; angiogenesis; response to axon injury; cell differentiation; positive regulation of granule cell precursor proliferation; positive regulation of cardiac muscle cell proliferation; fibroblast growth factor receptor signaling pathway; positive regulation of cell fate specification; positive regulation of blood vessel endothelial cell migration; positive regulation of phosphoinositide 3-kinase activity; negative regulation of blood vessel endothelial cell migration; positive regulation of osteoblast differentiation; positive regulation of protein kinase B signaling cascade; stem cell development; positive regulation of angiogenesis; cell migration during sprouting angiogenesis; ureteric bud branching; release of sequestered calcium ion into cytosol; positive regulation of cell division; phosphatidylinositol biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; positive regulation of cell differentiation; positive regulation of epithelial cell proliferation; inositol phosphate biosynthetic process; lung development
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Product References and Citations for bFGF recombinant protein

   expression of bFGF and il-1 alpha in tears of galactosemic rats: implications for wound healing and neovascularization .Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 545.
p53 is necessary for bFGF-induced lens differentiation .Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 4369.
regulation of basic fibroblast growing factor (bFGF) by retinal glial (müller) cells under severe retinal ischemia .Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 2926.
natural history of progressive experimental cnv in the rabbit after sustained release of both vegf and bFGF within the supra-choroidal space .Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 2970.
keratocyte-associated mt1-mmp modulates bFGF-induced vegf expression .Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 4967.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with bFGF recombinant proteinDiseases associated with bFGF recombinant protein
 Products by Pathway  Pathway Diagram
 Downstream Signaling Of Activated FGFR Pathway antibodies  Downstream Signaling Of Activated FGFR Pathway Diagram
 ESC Pluripotency Pathways antibodies  ESC Pluripotency Pathways Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 FGFR Ligand Binding And Activation Pathway antibodies  FGFR Ligand Binding And Activation Pathway Diagram
 FGFR1 Ligand Binding And Activation Pathway antibodies  FGFR1 Ligand Binding And Activation Pathway Diagram
 FGFR1b Ligand Binding And Activation Pathway antibodies  FGFR1b Ligand Binding And Activation Pathway Diagram
 FGFR1c Ligand Binding And Activation Pathway antibodies  FGFR1c Ligand Binding And Activation Pathway Diagram
 FGFR2 Ligand Binding And Activation Pathway antibodies  FGFR2 Ligand Binding And Activation Pathway Diagram
 FGFR2c Ligand Binding And Activation Pathway antibodies  FGFR2c Ligand Binding And Activation Pathway Diagram
 FGFR3 Ligand Binding And Activation Pathway antibodies  FGFR3 Ligand Binding And Activation Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >2369 publications with bFGF and Neoplasms
 Neovascularization, Pathologic Antibodies  >1187 publications with bFGF and Neovascularization, Pathologic
 Vascular Diseases Antibodies  >1020 publications with bFGF and Vascular Diseases
 Cardiovascular Diseases Antibodies  >892 publications with bFGF and Cardiovascular Diseases
 Inflammation Antibodies  >338 publications with bFGF and Inflammation
 Adenocarcinoma Antibodies  >320 publications with bFGF and Adenocarcinoma
 Myocardial Ischemia Antibodies  >307 publications with bFGF and Myocardial Ischemia
 Fibrosis Antibodies  >291 publications with bFGF and Fibrosis
 Neoplasms, Experimental Antibodies  >289 publications with bFGF and Neoplasms, Experimental
 Breast Neoplasms Antibodies  >263 publications with bFGF and Breast Neoplasms
Organs/Tissues associated with bFGF recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >904 publications with bFGF and Eye
 Stomach Antibodies  >116 publications with bFGF and Stomach
 Inner Ear Antibodies  >67 publications with bFGF and Inner Ear
 Mammary Gland Antibodies  >32 publications with bFGF and Mammary Gland
 Extraembryonic Tissue Antibodies  >15 publications with bFGF and Extraembryonic Tissue
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