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eno1 recombinant protein

Scan QR to view Datasheet Catalog #    MBS505003
Unit / Price
0.1 mg  /  $355 +1 FREE 8GB USB
0.5 mg  /  $590 +1 FREE 8GB USB
1 mg  /  $820 +1 FREE 8GB USB
5 mg  /  $3,175 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

eno1, Recombinant Protein

 Also Known As   

eno1 recombinant

 Product Synonym Names    RECOMBINANT ANTIGEN enolase from Candida albicans; enolase
 Product Gene Name   

eno1 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 3D Structure    ModBase 3D Structure for P30575
 Host    E Coli
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 Purity/Purification    0.98
 Form/Format    Liquid protein solution
 Concentration    0.59 mg/ml (lot specific)
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 Recombinant Antigen    Candida albicans enolase (Eroles et al., 1997).
 Organism    Candida albicans
 Tag    HIS
 Molecular Weight Information    Determined by SDS-PAGE, theprotein band is between molecular markers of 66,200-45,000 Da, while relative molecular mass calculated fromamino acid sequence is 56,543.7 Da.
 Observation Notes    Proteins should be maintained frozenat high concentrations. The dilution to be performed forELISA assays should be made with a small quantity ofprotein, the same day of the experiment. In order to defrost the protein, maintain the aliquot at 25 degree C without shaking toavoid aggregation. Prior making test dilutions and afterdefrost the protein, is recommended to remove possibleprotein aggregates by centrifuging the stock solution,avoiding alterations in the immobilization of the biomoleculeto the solid surface
 Buffer    20 mM phosphate buffer pH 8, 10 mM NaCl, 0.1% polyoxyethylene (10) tridecyl ether and 250 mM imidazol
 Batch Composition    Recombinant antigen with a his-tag in its N-terminus
 Storage Buffer    20 mM phosphate buffer pH 8, 10 mM NaCl, 0.1% polyoxyethylene (10) tridecyl ether and 250 mM imidazol
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 Preparation and Storage    Protein is shipped with dry ice. Upon arrival,it should be aliquoted in order to avoid repeated freezingand thawing cycles and stored at -20 degree C to -80 degree C
 Other Notes    Small volumes of eno1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for eno1 recombinant protein

   the Candida albicans enolase gene has been expressed as a recombinant antigen fused to a his-tag in its N-terminus. It is produced from the complete ORF of the 2-phospho-D-glyceratehydro-lyase.
 Product Categories/Family for eno1 recombinant protein    FUNGI
 Applications Tested/Suitable for eno1 recombinant protein   

ELISA (EIA), Western Blot (WB), Lateral Flow (LF)

 Application Notes for eno1 recombinant protein    Recommended concentration in ELISA plates: 0.39 ug/ml in plates for IgG detection
Suggested Titer by ELISA: 1:1500, which corresponds to 0.39 g/ml in plates for IgG detection.
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 Testing Data of eno1 recombinant protein    eno1 recombinant protein Testing Data image
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 Testing Data #2 of eno1 recombinant protein    eno1 recombinant protein Testing Data #2 image
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NCBI/Uniprot data below describe general gene information for eno1. It may not necessarily be applicable to this product.
 NCBI GI #    170861
 NCBI Accession #    AAB46358.1 [Other Products]
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 UniProt Primary Accession #    P30575 [Other Products]
 UniProt Secondary Accession #    Q59QC3 [Other Products]
 Molecular Weight    47,232 Da
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 NCBI Official Full Name    enolase
 UniProt Protein Name    Enolase 1
 UniProt Synonym Protein Names   
2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase
 Protein Family    Enolase
 UniProt Gene Name    ENO1 [Similar Products]
 UniProt Entry Name    ENO1_CANAL
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 UniProt Comments for eno1    Catalytic activity: 2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactor: Magnesium. Required for catalysis and for stabilizing the dimer.

Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.

Subunit structure: Homodimer

By similarity.

Subcellular location: Cytoplasm

By similarity.

Sequence similarities: Belongs to the enolase family.
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Product References and Citations for eno1 recombinant protein

   Eroles, P., Sentandreu, M., Elorza, M.V. andSentandreu, R. The highly immunogenic enolase andHSP70 are adventitious Candida albicans cell Wall proteins.1997, Microbiology, 143:313-20.
Gill SC, von Hippel PH. Calculation of protein extinctioncoefficients from amino acid sequence data. AnalBiochem. 1989 Nov 1;182(2):319-26.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with eno1 recombinant protein
 Reference Product  PubMed Publications
 PGK1 recombinant protein  >10 publications with eno1 and PGK1
 TPI1 recombinant protein  >8 publications with eno1 and TPI1
 FBA1 recombinant protein  >5 publications with eno1 and FBA1
 TDH1 recombinant protein  >2 publications with eno1 and TDH1
 PYK1 recombinant protein  >2 publications with eno1 and PYK1
 GPM1 recombinant protein  >1 publications with eno1 and GPM1
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