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fre recombinant protein :: k12 NAD(P)H-flavin reductase Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS1265545
SDS-PAGE
Unit / Price
0.01 mg  /  $110 +1 FREE 8GB USB
0.05 mg  /  $190 +1 FREE 8GB USB
0.1 mg  /  $285 +1 FREE 8GB USB
0.2 mg  /  $460 +1 FREE 8GB USB
0.5 mg  /  $750 +1 FREE 8GB USB
1 mg  /  $1,180 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

k12 NAD(P)H-flavin reductase (fre), Recombinant Protein

 Also Known As   

Recombinant E Coli-k12 NAD(P)H-flavin reductase

 Product Synonym Names    Aquacobalamin reductase; FMN reductase; Ferrisiderophore reductase CNAD(P)H:flavin oxidoreductase; Riboflavin reductase [NAD(P)H]
 Product Gene Name   

fre recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence Positions    2-233aa; Full Length
 Sequence    TTLSCKVTSV EAITDTVYRV RIVPDAAFSF RAGQYLMVVM DERDKRPFSM ASTPDEKGFI ELHIGASEIN LYAKAVMDRI LKDHQIVVDI PHGEAWLRDD EERPMILIAG GTGFSYARSI LLTALARNPN RDITIYWGGR EEQHLYDLCE LEALSLKHPG LQVVPVVEQP EAGWRGRTGT VLTAVLQDHG TLAEHDIYIA GRFEMAKIAR DLFCSERNAR EDRLFGDAFA FI
 3D Structure    ModBase 3D Structure for P0AEN1
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 Host    E Coli
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 Purity/Purification    Greater than 90% as determined by SDS-PAGE. (lot specific)
 Form/Format    Liquid containing glycerol
 Tag Information    This protein contains an N-terminal tag and may also contain a C-terminal Myc-tag. N-terminal host tags may vary (His, His-SUMO, His-B2M, GST). Tag types are determined by various factors including tag-protein stability and, therefore, are subject to change; please inquire for tag information.
 Sterility    Sterile filter available upon request.
 Endotoxin    Low endotoxin available upon request.
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 Preparation and Storage    Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of fre recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for fre recombinant protein

   Catalyzes the reduction of soluble flavins by reduced pyridine nucleotides. Ses to reduce the complexed Fe3+ iron of siderophores to Fe2+, thus releasing it from the chelator.
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 SDS-PAGE of fre recombinant protein    fre recombinant protein SDS-PAGE image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
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NCBI/Uniprot data below describe general gene information for fre. It may not necessarily be applicable to this product.
 NCBI GI #    16131690
 NCBI GeneID    948325
 NCBI Accession #    NP_418286.1 [Other Products]
 NCBI GenBank Nucleotide #    NC_000913.3 [Other Products]
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 UniProt Primary Accession #    P0AEN1 [Other Products]
 UniProt Secondary Accession #    P23486; P76768; Q2M8E7 [Other Products]
 Molecular Weight    30.2kD [Similar Products]
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 NCBI Official Full Name    NAD(P)H-flavin reductase
 NCBI Official Symbol    fre [Similar Products]
 NCBI Official Synonym Symbols   
ECK3836; fadI; flrD; fsrC; hsm-6; JW3820
[Similar Products]
 NCBI Protein Information    NAD(P)H-flavin reductase
 UniProt Protein Name    NAD(P)H-flavin reductase
 UniProt Synonym Protein Names   
Aquacobalamin reductase; FMN reductase; Ferrisiderophore reductase C; NAD(P)H:flavin oxidoreductase; Riboflavin reductase [NAD(P)H]
 Protein Family    NAD(P)H-flavin reductase
 UniProt Gene Name    fre [Similar Products]
 UniProt Synonym Gene Names    fadI; flrD; fsrC; ubiB [Similar Products]
 UniProt Entry Name    FRE_ECOLI
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 NCBI Summary for fre    The fre gene was mistakenly thought to be ubiB. [More information is available at EcoGene: EG10334].
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 UniProt Comments for fre    Catalyzes the reduction of soluble flavins by reduced pyridine nucleotides. Seems to reduce the complexed Fe3+ iron of siderophores to Fe2+, thus releasing it from the chelator.
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Product References and Citations for fre recombinant protein

   Characterization of the flavin reductase gene (fre) of Escherichia coli and construction of a plasmid for overproduction of the enzyme.Spyrou G., Haggaard-Ljungquist E., Krook M., Joernvall H., Nilsson E., Reichard P.J. Bacteriol. 173:3673-3679(1991) FadI identification, characterization, and nucleotide sequence of a gene required for full activation of structural genes of the AD regulon in Escherichia coli.Dirusso C.C., Shea O. Saviranta P.J. Analysis of the Escherichia coli genome DNA sequence of the region from 84.5 to 86.5 minutes.Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.Science 257:771-778(1992) The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) The haemoglobin-like protein (HMP) of Escherichia coli has ferrisiderophore reductase activity and its C-terminal domain shares homology with ferredoxin NADP+ reductases.Andrews S.C., Shipley D., Keen J.N., Findlay J.B.C., Harrison P.M., Guest J.R.FEBS Lett. 302:247-252(1992) Crystal structure of NAD(P) H:flavin oxidoreductase from Escherichia coli.Ingelman M., Ramaswamy S., Niviere V., Fontecave M., Eklund H.Biochemistry 38:7040-7049(1999)
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 Research Articles on fre    1. N-terminus verified by Edman degradation on mature peptide
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with fre recombinant proteinPathways associated with fre recombinant protein
 Reference Product  PubMed Publications
 pyrB recombinant protein  >1 publications with fre and pyrB
 aphA recombinant protein  >1 publications with fre and aphA
 Products by Pathway  Pathway Diagram
 Porphyrin And Chlorophyll Metabolism Pathway antibodies  Porphyrin And Chlorophyll Metabolism Pathway Diagram
 Porphyrin And Chlorophyll Metabolism Pathway antibodies  Porphyrin And Chlorophyll Metabolism Pathway Diagram
 Riboflavin Metabolism Pathway antibodies  Riboflavin Metabolism Pathway Diagram
 Riboflavin Metabolism Pathway antibodies  Riboflavin Metabolism Pathway Diagram
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