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LEP recombinant protein :: leptin antagonist Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS400036 LEP recombinant protein
Unit / Price
0.05 mg  /  $145 +1 FREE 8GB USB
0.1 mg  /  $175 +1 FREE 8GB USB
0.5 mg  /  $515 +1 FREE 8GB USB
1 mg  /  $785 +1 FREE 8GB USB
5 mg  /  $2,910 +3 FREE 8GB USB
10 mg  /  $5,015 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

leptin antagonist, Recombinant Protein

 Also Known As   

Ovine leptin antagonist (mutant L39A/D40A/F41A)

 Product Gene Name   

LEP recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    The sequence of the first five N-terminal amino acids of ovine recombinan t leptin antagonist was determined and was found to be Ala-Val-Pr o-Ile-Arg
 OMIM    164160
 3D Structure    ModBase 3D Structure for P41159
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 Host    Escherichia Coli
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 Purity/Purification    The purity of ovine recombinant leptin antagonist is greater than 98.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
 Form/Format    White lyophilized (freeze-dried) powder.
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 Solubility    It is recommended to reconstitute the lyophilized recombinant leptin antagonist in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
 Protein Content    Protein quantitation of ovine recombinant leptin antagonist was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.21 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
 Dimers and Aggregates    The purified ovine recombinant leptin antagonist (16K) consists of > 95% monomers as determined by gel-filtration chromatography.
 Endotoxin    The endotoxin content of ovine recombinant leptin antagonist is less than 0.1 ng/ug (IEU/ug) of ovine leptin antagonist.
 Formulation    The recombinant leptin antagonist was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.
 Biological Activity    ovine recombinant leptin antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of ovine leptin receptor. It also inhibits various leptin effects in several in vitro bioassays.
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 Preparation and Storage    Lyophilized ovine recombinant leptin antagonist although stable at room temperature for several weeks, should be stored desiccated below -18 degree C. Upon reconstitution of ovine recombinant leptin antagonist at > 0.1 mg/ml and up to 2 mg and filter sterilization ovine recombinant leptin antagonist can be stored at +4 degree C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.
 Other Notes    Small volumes of LEP recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for LEP recombinant protein

   Recombinant ovine leptin, one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa, oLEP was mutated, resulting in L39A/D40A/F41A mutant was purified by proprietary chromatographic techniques. Preparation of recombinant leptin antagonists was recently published - Niv-Spector et al, Biochem. J 291;221-230 (2005).
 Product Categories/Family for LEP recombinant protein    Leptin and leptin muteins
NCBI/Uniprot data below describe general gene information for LEP. It may not necessarily be applicable to this product.
 NCBI GI #    4557715
 NCBI GeneID    3952
 NCBI Accession #    NP_000221.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000230.2 [Other Products]
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 UniProt Primary Accession #    P41159 [Other Products]
 UniProt Secondary Accession #    O15158; Q56A88 [Other Products]
 UniProt Related Accession #    P41159 [Other Products]
 Molecular Weight    18,641 Da [Similar Products]
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 NCBI Official Full Name    leptin
 NCBI Official Synonym Full Names    leptin
 NCBI Official Symbol    LEP [Similar Products]
 NCBI Official Synonym Symbols   
OB; OBS; LEPD
[Similar Products]
 NCBI Protein Information    leptin; leptin (murine obesity homolog); leptin (obesity homolog, mouse); obese protein; obese, mouse, homolog of; obesity factor
 UniProt Protein Name    Leptin
 UniProt Synonym Protein Names   
Obese protein; Obesity factor
 Protein Family    Leptin
 UniProt Gene Name    LEP [Similar Products]
 UniProt Synonym Gene Names    OB; OBS [Similar Products]
 UniProt Entry Name    LEP_HUMAN
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 NCBI Summary for LEP    This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Jul 2008]
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 UniProt Comments for LEP    leptin: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Defects in LEP may be a cause of obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Belongs to the leptin family.

Protein type: Hormone; Secreted, signal peptide; Cell development/differentiation; Secreted

Chromosomal Location of Human Ortholog: 7q31.3

Cellular Component: extracellular space; cytoplasm; extracellular region

Molecular Function: peptide hormone receptor binding; growth factor activity; hormone activity

Biological Process: circadian rhythm; response to dietary excess; positive regulation of myeloid cell differentiation; regulation of fat cell differentiation; regulation of steroid biosynthetic process; female pregnancy; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; negative regulation of appetite; positive regulation of luteinizing hormone secretion; response to insulin stimulus; positive regulation of tyrosine phosphorylation of Stat3 protein; response to vitamin E; regulation of cholesterol absorption; positive regulation of MAPKKK cascade; regulation of blood pressure; positive regulation of cell proliferation; positive regulation of ion transport; central nervous system neuron development; placenta development; positive regulation of cytokine production; cholesterol metabolic process; positive regulation of developmental growth; eating behavior; bile acid metabolic process; glucose metabolic process; adult feeding behavior; ovulation from ovarian follicle; leptin-mediated signaling pathway; negative regulation of vasoconstriction; tyrosine phosphorylation of STAT protein; fatty acid beta-oxidation; glycerol biosynthetic process; insulin secretion; response to hypoxia; energy reserve metabolic process; hormone metabolic process; regulation of gluconeogenesis; positive regulation of insulin receptor signaling pathway; positive regulation of follicle-stimulating hormone secretion; leukocyte tethering or rolling; regulation of insulin secretion; negative regulation of apoptosis

Disease: Leptin Deficiency
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 Research Articles on LEP    1. The difference of leptin levels between control and obese groups are more likely to be associated with alterations in the leptin gene other than 25th codon or alterations in the leptin receptor gene.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with LEP recombinant proteinPathways associated with LEP recombinant protein
 Reference Product  PubMed Publications
 PPARG recombinant protein  >38 publications with LEP and PPARG
 STAT3 recombinant protein  >16 publications with LEP and STAT3
 GHRL recombinant protein  >11 publications with LEP and GHRL
 IL6 recombinant protein  >10 publications with LEP and IL6
 IRS1 recombinant protein  >7 publications with LEP and IRS1
 JAK2 recombinant protein  >5 publications with LEP and JAK2
 PTPN1 recombinant protein  >2 publications with LEP and PTPN1
 SOCS3 recombinant protein  >1 publications with LEP and SOCS3
 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 HIF-1-alpha Transcription Factor Network Pathway antibodies  HIF-1-alpha Transcription Factor Network Pathway Diagram
Diseases associated with LEP recombinant proteinOrgans/Tissues associated with LEP recombinant protein
 Disease Name  Pubmed Publications
 Obesity Antibodies  >368 publications with LEP and Obesity
 Pain Antibodies  >177 publications with LEP and Pain
 Insulin Resistance Antibodies  >117 publications with LEP and Insulin Resistance
 Hyperinsulinism Antibodies  >78 publications with LEP and Hyperinsulinism
 Weight Gain Antibodies  >62 publications with LEP and Weight Gain
 Weight Loss Antibodies  >60 publications with LEP and Weight Loss
 Cardiovascular Diseases Antibodies  >53 publications with LEP and Cardiovascular Diseases
 Inflammation Antibodies  >51 publications with LEP and Inflammation
 Fatty Liver Antibodies  >46 publications with LEP and Fatty Liver
 Liver Diseases Antibodies  >42 publications with LEP and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >381 publications with LEP and Blood
 Adipose Tissue Antibodies  >173 publications with LEP and Adipose Tissue
 Connective Tissue Antibodies  >123 publications with LEP and Connective Tissue
 Muscle Antibodies  >120 publications with LEP and Muscle
 Placenta Antibodies  >30 publications with LEP and Placenta
 Intestine Antibodies  >21 publications with LEP and Intestine
 Mouth Antibodies  >9 publications with LEP and Mouth
 Mammary Gland Antibodies  >4 publications with LEP and Mammary Gland
 Thymus Antibodies  >3 publications with LEP and Thymus
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