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phrB recombinant protein

Scan QR to view Datasheet Catalog #    MBS205054
SDS-Page
Unit / Price
0.02 mg  /  $310 +1 FREE 8GB USB
0.1 mg  /  $720 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

phrB, Recombinant Protein

★Popular Item★
 Also Known As   

phrB, 1-472aa, E Coli, His tag, E Coli

 Product Synonym Names    Deoxyribodipyrimidine photolyase; ECK0697; JW0698; phrB
 Product Gene Name   

phrB recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MGSSHHHHHH SSGLVPRGSH MGSMTTHLVW FRQDLRLHDN LALAAACRNS SARVLALYIA TPRQWATHNM SPRQAELINA QLNGLQIALA EKGIPLLFRE VDDFVASVEI VKQVCAENSV THLFYNYQYE VNERARDVEV ERALRNVVCE GFDDSVILPP GAVMTGNHEM YKVFTPFKNA WLKRLREGMP ECVAAPKVRS SGSIEPSPSI TLNYPRQSFD TAHFPVEEKA AIAQLRQFCQ NGAGEYEQQR DFPAVEGTSR LSASLATGGL SPRQCLHRLL AEQPQALDGG AGSVWLNELI WREFYRHLIT YHPSLCKHRP FIAWTDRVQW QSNPAHLQAW QEGKTGYPIV DAAMRQLNST GWMHNRLRMI TASFLVKDLL IDWREGERYF MSQLIDGDLA ANNGGWQWAA STGTDAAPYF RIFNPTTQGE KFDHEGEFIR QWLPELRDVP GKVVHEPWKW AQKAGVTLDY PQPIVEHKEA RVQTLAAYEA ARKGK
 3D Structure    ModBase 3D Structure for P00914
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 Host    E Coli
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 Purity/Purification    > 90% by SDS-PAGE
 Form/Format    Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 1mM DTT
 Concentration    0.25 mg/ml (determined by Bradford assay) (lot specific)
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 Antigen Species    E Coli,
 Tag Information    His-tag
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 Preparation and Storage    Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of phrB recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for phrB recombinant protein

   Deoxyribodipyrimidine photolyase, also known as phrB, is involved in repair of uV radiation-induced DNA damage. This protein catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ultraviolet radiation. Recombinant E Coli phrB protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
 Product Categories/Family for phrB recombinant protein    Enzymes & Proteases
 Applications Tested/Suitable for phrB recombinant protein   

SDS-PAGE

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 SDS-Page of phrB recombinant protein    phrB recombinant protein SDS-Page image
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NCBI/Uniprot data below describe general gene information for phrB. It may not necessarily be applicable to this product.
 NCBI GI #    16128683
 NCBI GeneID    947005
 NCBI Accession #    NP_415236 [Other Products]
 NCBI GenBank Nucleotide #    NC_000913.3 [Other Products]
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 UniProt Primary Accession #    P00914 [Other Products]
 Molecular Weight    56.1 kDa (495aa)
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 NCBI Official Full Name    deoxyribodipyrimidine photolyase, FAD-binding
 NCBI Official Symbol    phr [Similar Products]
 NCBI Official Synonym Symbols   
ECK0697; JW0698; phrB
[Similar Products]
 NCBI Protein Information    deoxyribodipyrimidine photolyase, FAD-binding
 UniProt Protein Name    Deoxyribodipyrimidine photo-lyase
 UniProt Synonym Protein Names   
DNA photolyase; Photoreactivating enzyme
 Protein Family    (6-4) photolyase
 UniProt Gene Name    phrB [Similar Products]
 UniProt Synonym Gene Names    phr [Similar Products]
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 NCBI Summary for phrB    The flavoprotein DNA photolyase uses light to repair cyclobutylpyrimidine dimers in DNA. [More information is available at EcoGene: EG10736]. Exposing Escherichia coli to UV radiation at wavelengths near the absorption maximum of DNA produces cyclobutane-type pyrimidine dimers (CPDs) as well as, to a lesser degree, 6-4 pyrimidine-pyrimidones in its DNA. [More information is available at EcoCyc: EG10736].
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 UniProt Comments for phrB    Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ultraviolet radiation.
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Product References and Citations for phrB recombinant protein

   Byrdin M., et al. (2003) Proc. Natl. Acad. Sci. u.S.A. 100:8676-8681; Weber S., et al. (2005) Biochim. Biophys. Acta. 1707:1-23;
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 Research Articles on phrB    1. Data show that DNA photolyase exhibits a distinct reduction-potential gradient along the same aromatic residues with favorable reorganization energies to drive a highly unidirectional electron flow toward the active-site center from the protein surface.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with phrB recombinant protein
 Reference Product  PubMed Publications
 uvrB recombinant protein  >9 publications with phrB and uvrB
 phrB recombinant protein  >3 publications with phrB and phrB
 bluR recombinant protein  >1 publications with phrB and bluR
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