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uGDH recombinant protein

Scan QR to view Datasheet Catalog #    MBS203653
SDS-PAGE
Unit / Price
0.05 mg  /  $310 +1 FREE 8GB USB
0.25 mg  /  $720 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

uGDH, Recombinant Protein

★Popular Item★
 Also Known As   

uGDH, 1-494aa, Human, His tag, E Coli

 Product Synonym Names    GDH; uDP-GlcDH; uDPGDH; uGD; uDP-glucose dehydrogenase; uDP-glucose 6-dehydrogenase
 Product Gene Name   

uGDH recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELEM FEIKKICCIG AGYVGGPTCS VIAHMCPEIR VTVVDVNESR INAWNSPTLP IYEPGLKEVV ESCRGKNLFF STNIDDAIKE ADLVFISVNT PTKTYGMGKG RAADLKYIEA CARRIVQNSN GYKIVTEKST VPVRAAESIR RIFDANTKPN LNLQVLSNPE FLAEGTAIKD LKNPDRVLIG GDETPEGQRA VQALCAVYEH WVPREKILTT NTWSSELSKL AANAFLAQRI SSINSISALC EATGADVEEV ATAIGMDQRI GNKFLKASVG FGGSCFQKDV LNLVYLCEAL NLPEVARYWQ QVIDMNDYQR RRFASRIIDS LFNTVTDKKI AILGFAFKKD TGDTRESSSI YISKYLMDEG AHLHIYDPKV PREQIVVDLS HPGVSEDDQV SRLVTISKDP YEACDGAHAV VICTEWDMFK ELDYERIHKK MLKPAFIFDG RRVLDGLHNE LQTIGFQIET IGKKVSSKRI PYAPSGEIPK FSLQDPPNKK PKV
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 Purity/Purification    > 95 % by SDS-PAGE
 Form/Format    Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 20% glycerol, 1mM DTT, 0.1M NaCl, and 1mM EDTA.
 Concentration    0.5 mg/ml (determined by Bradford assay) (lot specific)
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 Antigen Species    Human
 Tag    His-tag
 Expression System    E Coli
 Bioactivity    Specific activity: > 0.1 unit/ml. One unit will oxidize 1.0 umol of uDP-glucose to uDP-glucuronic acid per minute at pH 8.0 at 25C
 Assay    1. Prepare a 1470 ul assay buffer. The final concentrations are 93mM Tris-Hcl (pH 8.0), 0.2mM uDPG, and 1mM beta-NAD.
2. Add 20 ul of recombinant protein uGDH with 1 ug, 2 ug and 5 ug in assay buffer
3. Mix by inversion and load 200 ul of reaction mix in to a plate well.
4. Record the decrease in A340nm for 5 minutes at 25C
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 Preparation and Storage    Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of uGDH recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for uGDH recombinant protein

   uGDH is a member of the uDP-glucose/GDP-mannose dehydrogenase family and is a ubiquitously expressed protein most abundant in the liver. This protein converts uDP-glucose to uDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis. Recombinant human uGDH protein, fused to his-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques
 Product Categories/Family for uGDH recombinant protein    Enzymes & Proteases
 Applications Tested/Suitable for uGDH recombinant protein   

SDS-PAGE

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 SDS-PAGE of uGDH recombinant protein    uGDH recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for uGDH. It may not necessarily be applicable to this product.
 NCBI GI #    4507813
 NCBI GeneID    7358
 NCBI Accession #    NP_003350 [Other Products]
 NCBI GenBank Nucleotide #    NM_003359.3 [Other Products]
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 UniProt Secondary Accession #    O60589; B3KUU2; B4DN25 [Other Products]
 UniProt Related Accession #    O60701 [Other Products]
 Molecular Weight    59.5 kDa (533aa) confirmed by MALDI-TOF
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 NCBI Official Full Name    UDP-glucose 6-dehydrogenase isoform 1
 NCBI Official Synonym Full Names    UDP-glucose 6-dehydrogenase
 NCBI Official Symbol    UGDH [Similar Products]
 NCBI Official Synonym Symbols   
GDH; UGD; UDPGDH; UDP-GlcDH
[Similar Products]
 NCBI Protein Information    UDP-glucose 6-dehydrogenase; UDP-Glc dehydrogenase; UDP-glucose dehydrogenase; uridine diphospho-glucose dehydrogenase
 UniProt Protein Name    UDP-glucose 6-dehydrogenase
 UniProt Gene Name    UGDH [Similar Products]
 UniProt Synonym Gene Names    UDP-Glc dehydrogenase; UDP-GlcDH; UDPGDH [Similar Products]
 UniProt Entry Name    UGDH_HUMAN
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 NCBI Summary for uGDH    The protein encoded by this gene converts UDP-glucose to UDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis. The expression of this gene is up-regulated by transforming growth factor beta and down-regulated by hypoxia. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
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 UniProt Comments for uGDH    UGDH: Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate, and heparan sulfate. Belongs to the UDP-glucose/GDP-mannose dehydrogenase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - pentose and glucuronate interconversions; Oxidoreductase; Carbohydrate Metabolism - amino sugar and nucleotide sugar; EC 1.1.1.22; Carbohydrate Metabolism - starch and sucrose; Carbohydrate Metabolism - ascorbate and aldarate; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4p15.1

Cellular Component: nucleoplasm; cytosol

Molecular Function: electron carrier activity; UDP-glucose 6-dehydrogenase activity; NAD binding

Biological Process: glycosaminoglycan biosynthetic process; xenobiotic metabolic process; gastrulation with mouth forming second; UDP-glucuronate biosynthetic process; UDP-glucose metabolic process
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Product References and Citations for uGDH recombinant protein

   Bontemps Y., et al. (2003) J Biol Chem. 278(24):21566-75.
Vatsyayan J., et al. (2006) Biosci Biotechnol Biochem. 70(2):401-10.
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 Research Articles on uGDH    1. Kinetic analysis of wild-type UGDH and hexamer T325A showed that upon increasing enzyme concentration, which favors the hexameric species, activity was decreased and exhibited cooperativity. Cooperative kinetics was not observed in obligate dimer T325D.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with uGDH recombinant proteinPathways associated with uGDH recombinant protein
 Reference Product  PubMed Publications
 UGT1A1 recombinant protein  >1 publications with uGDH and UGT1A1
 UGP2 recombinant protein  >1 publications with uGDH and UGP2
 Products by Pathway  Pathway Diagram
 Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies  Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
 Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies  Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
 Ascorbate And Aldarate Metabolism Pathway antibodies  Ascorbate And Aldarate Metabolism Pathway Diagram
 Ascorbate And Aldarate Metabolism Pathway antibodies  Ascorbate And Aldarate Metabolism Pathway Diagram
 Biological Oxidations Pathway antibodies  Biological Oxidations Pathway Diagram
 Defective AHCY Causes Hypermethioninemia With S-adenosylhomocysteine Hydrolase Deficiency (HMAHCHD) Pathway antibodies  Defective AHCY Causes Hypermethioninemia With S-adenosylhomocysteine Hydrolase Deficiency (HMAHCHD) Pathway Diagram
 Defective GCLC Causes Hemolytic Anemia Due To Gamma-glutamylcysteine Synthetase Deficiency (HAGGSD) Pathway antibodies  Defective GCLC Causes Hemolytic Anemia Due To Gamma-glutamylcysteine Synthetase Deficiency (HAGGSD) Pathway Diagram
 Defective GGT1 Causes Glutathionuria (GLUTH) Pathway antibodies  Defective GGT1 Causes Glutathionuria (GLUTH) Pathway Diagram
 Defective GSS Causes Glutathione Synthetase Deficiency (GSS Deficiency) Pathway antibodies  Defective GSS Causes Glutathione Synthetase Deficiency (GSS Deficiency) Pathway Diagram
 Defective MAT1A Causes Methionine Adenosyltransferase Deficiency (MATD) Pathway antibodies  Defective MAT1A Causes Methionine Adenosyltransferase Deficiency (MATD) Pathway Diagram
Diseases associated with uGDH recombinant proteinOrgans/Tissues associated with uGDH recombinant protein
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >4 publications with uGDH and Cardiovascular Diseases
 Heart Diseases Antibodies  >3 publications with uGDH and Heart Diseases
 Congenital Abnormalities Antibodies  >3 publications with uGDH and Congenital Abnormalities
 Liver Diseases Antibodies  >2 publications with uGDH and Liver Diseases
 Nervous System Diseases Antibodies  >2 publications with uGDH and Nervous System Diseases
 Neoplasm Metastasis Antibodies  >1 publications with uGDH and Neoplasm Metastasis
 Drug-Related Side Effects and Adverse Reactions Antibodies  >1 publications with uGDH and Drug-Related Side Effects and Adverse Reactions
 Edema Antibodies  >1 publications with uGDH and Edema
 Poisoning Antibodies  >1 publications with uGDH and Poisoning
 Carcinoma, Hepatocellular Antibodies  >1 publications with uGDH and Carcinoma, Hepatocellular
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >7 publications with uGDH and Liver
 Muscle Antibodies  >6 publications with uGDH and Muscle
 Prostate Antibodies  >3 publications with uGDH and Prostate
 Heart Antibodies  >3 publications with uGDH and Heart
 Bone Antibodies  >3 publications with uGDH and Bone
 Lung Antibodies  >2 publications with uGDH and Lung
 Embryonic Tissue Antibodies  >2 publications with uGDH and Embryonic Tissue
 Kidney Antibodies  >2 publications with uGDH and Kidney
 Brain Antibodies  >2 publications with uGDH and Brain
 Blood Antibodies  >1 publications with uGDH and Blood
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