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uPK3A recombinant protein

Scan QR to view Datasheet Catalog #    MBS204417
SDS-PAGE
Unit / Price
0.05 mg  /  $375 +1 FREE 8GB USB
0.25 mg  /  $925 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

uPK3A, Recombinant Protein

 Also Known As   

uPK3A, 19-207aa, Human, His tag, E Coli

 Product Synonym Names    uroplakin-3a; uPK3
 Product Gene Name   

uPK3A recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MGSSHHHHHH SSGLVPRGSH MGSHMVNLQP QLASVTFATN NPTLTTVALE KPLCMFDSKE ALTGTHEVYL YVLVDSAISR NASVQDSTNT PLGSTFLQTE GGRTGPYKAV AFDLIPCSDL PSLDAIGDVS KASQILNAYL VRVGANGTCL WDPNFQGLCN PPLSAATEYR FKYVLVNMST GLVEDQTLWS DPIRTNQLTP YSTIDTWPGR RSGG
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 Purity/Purification    > 90% by SDS - PAGE
 Form/Format    Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 150mM NaCl
 Concentration    0.25mg/ml (determined by Bradford assay) (lot specific)
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 Antigen Species    Human
 Tag    His-tag
 Expression System    E Coli
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 Preparation and Storage    Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of uPK3A recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for uPK3A recombinant protein

   uPK3A belongs to the uroplakin-3 family. uroplakins (uP) are key components of the urothelium. They are transmembrane proteins that pass the lipid bilayer once (uPII, uPIIIa, and uPIIIb) or four times (uPIa and uPIb; both belonging to the 'tetraspanin' family). All have large luminal/extracellular domains, but only uPIIIa and uPIIIb have significant cytoplasmic portions in their C-termini. uPK3A is a highly specific and moderately sensitive immunohistochemical marker for primary and metastatic urothelial carcinomas. Mutations in this gene may be associated with renal adysplasia Recombinant human uPK3A protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
 Product Categories/Family for uPK3A recombinant protein    Cancer
 Applications Tested/Suitable for uPK3A recombinant protein   

SDS-PAGE

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 SDS-PAGE of uPK3A recombinant protein    uPK3A recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for uPK3A. It may not necessarily be applicable to this product.
 NCBI GI #    3298345
 NCBI Accession #    BAA31460 [Other Products]
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 UniProt Secondary Accession #    O60261; Q32N05; Q5TII6; B0QY25 [Other Products]
 Molecular Weight    23.1kDa (214aa), confirmed by MALDI-TOF
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 NCBI Official Full Name    uroplkin 3
 UniProt Protein Name    Uroplakin-3a
 UniProt Synonym Protein Names   
Uroplakin III; UPIII
 Protein Family    Uroplakin
 UniProt Gene Name    UPK3A [Similar Products]
 UniProt Synonym Gene Names    UPK3; UP3a; UPIII [Similar Products]
 UniProt Entry Name    UPK3A_HUMAN
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 UniProt Comments for uPK3A    UPK3A: Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells. It also contributes to the formation of urothelial glycocalyx which may play an important role in preventing bacterial adherence. Defects in UPK3A are a cause of renal adysplasia (RADYS); also known as renal agenesis or renal aplasia. Renal agenesis refers to the absence of one (unilateral) or both (bilateral) kidneys at birth. Bilateral renal agenesis belongs to a group of perinatally lethal renal diseases, including severe bilateral renal dysplasia, unilateral renal agenesis with contralateral dysplasia and severe obstructive uropathy. Belongs to the uroplakin-3 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: endoplasmic reticulum membrane; apical plasma membrane; integral to membrane

Biological Process: epithelial cell differentiation; urinary bladder development; water transport; urea transport; cell morphogenesis; sodium ion homeostasis; kidney development; potassium ion homeostasis

Disease: Renal Hypodysplasia/aplasia 1
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Product References and Citations for uPK3A recombinant protein

   Shapiro, E., et al. (2000) J. urol. 164: 1048-1051.
Wu, X.R., et al. (1994) J. Biol. Chem. 269:13716-13724.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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