View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Add To Compare List 10 nm Colloidal Gold Conjugated Goat Anti-rabbit IgG Secondary Antibody Catalog # MBS176621 Unit / Price DISCONTINUEDClick here to view our similar product(s) OR click here to contact our technical support team for suitable replacement. Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name 10 nm Colloidal Gold Conjugated Goat Anti-rabbit IgG, Secondary Antibody Popular Item Full Product Name 10 nm Colloidal Gold Conjugated Goat Anti-rabbit IgG secondary antibody Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Polyclonal Isotype IgG Host Goat Specificity This colloidal gold conjugated antibody is specific for rabbit IgG and shows no cross-reactivity with mouse, bovine IgG. Form/Format Concentrated, Liquid; 0.1 mg of colloidal gold conjugated specific antibody, the diameter of colloidal gold is 10±2 nm, 0.01 M PBS, 0.01% Thimerosal, 50% glycerol. Immunogen Rabbit IgG (whole molecule). Preparation of Diluent Buffer The conjugate should be diluted for most applications, using 0.01M PBS or TBS, containing 1% BSA.Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl, 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid into 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.Preparation of 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 into 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L. Preparation and Storage At 4°C for one year. ISO Certification Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory. Other Notes Small volumes of 10 nm Colloidal Gold Conjugated Goat Anti-rabbit IgG secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for 10 nm Colloidal Gold Conjugated Goat Anti-rabbit IgG secondary antibody Description:This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for rabbit IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity in tissue or cell preparations. The goat anti-rabbit IgG is conjugated to 10 nm colloidal gold and generates electron dense particles that are visible using electron microscopy.Background:Immunoglobulin G (IgG) is the most common type of antibody found in the circulation. It is a protein complex composed of four peptide chainstwo identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. IgG molecules are created and released by plasma B cells. By binding many kinds of pathogens such as viruses, bacteria, and fungi, IgG protects the body from infection. Applications Tested/Suitable for 10 nm Colloidal Gold Conjugated Goat Anti-rabbit IgG secondary antibody Electron Microscopy (EM), Immunohistochemistry-Paraffin-embedded / Frozen Section (IHC-P/Fr), Immunocytochemistry (ICC) Application Notes for 10 nm Colloidal Gold Conjugated Goat Anti-rabbit IgG secondary antibody Electron Microscopy: 1:20-50Immunohistochemistry (Paraffin-embedded Section): 1:50-100Immunohistochemistry (Frozen Section): 1:50-100Immunocytochemistry: 1:50-100Optimal working dilutions must be determined by end uses. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP