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5 nm Colloidal Gold Conjugated Goat Anti-mouse IgG Secondary Antibody

Scan QR to view Datasheet Catalog #    MBS176697 5 nm Colloidal Gold Conjugated Goat Anti-mouse IgG secondary antibody
Unit / Price
1 mL  /  $180 +1 FREE 8GB USB
 
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 Product Name   

5 nm Colloidal Gold Conjugated Goat Anti-mouse IgG, Secondary Antibody

★Popular Item★
 Also Known As   

5 nm Colloidal Gold Conjugated Goat Anti-mouse IgG

 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Goat
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 Specificity    This colloidal gold conjugated antibody is specific for mouse IgG and shows no cross-reactivity with human, bovine, rabbit IgG.
 Form/Format    Concentrated, Liquid
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 Immunogen    Mouse IgG (whole molecule).
 Contents    0.1 mg of colloidal gold conjugated specific antibody, the diameter of colloidal gold is 5±1 nm; 0.01 M PBS; 0.01 % Thimerosal, 50% glycerol.
 Preparation of Colloidal Gold    5 nm Colloidal Gold - Add 0.05% trisodium citrate and 0.05% Vitamin C into boiled 0.01 % chloroauric acid which was reduced to gold granule solution through redox reaction.
Conjugate colloidal gold to antibody after rnensurating the optimal data. And through overspeed centrifugation and gradient delamination to density, the colloidal gold conjugated reagents were produced.
 Preparation of Diluent Buffer    The conjugate should be diluted for most applications, using 0.01M PBS or TBS, containing 1% BSA. Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl, 450?l of purified acetic acid or 700?l of concentrated hydrochloric acid into 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L. Preparation of 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 into 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L
 Preparation of IGSS (ImmunoGold Silver Staining)    Add citric acid 2.35g, trisodium citrate 2.55g, glutin 19 and hydroquinone 1.7g into 100 ml of distilled water and mix evenly. Before usage, add silver nitrate 50mg into it. Then, add this mixed solution into tissue at last and colorate for 10-20 minutes in dark. Wash with water to terminate reaction .
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 Preparation and Storage    At 4 degree C for one year.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of 5 nm Colloidal Gold Conjugated Goat Anti-mouse IgG secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for 5 nm Colloidal Gold Conjugated Goat Anti-mouse IgG secondary antibody

   Description: immunoglobulin G (IgG) is the most common type of antibody found in the circulation. It is a protein complex composed of four peptide chains—two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. IgG molecules are created and released by plasma B cells. By binding many kinds of pathogens such as viruses, bacteria, and fungi, IgG protects the body from infection
 Applications Tested/Suitable for 5 nm Colloidal Gold Conjugated Goat Anti-mouse IgG secondary antibody   

Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry (ICC), EM

 Application Notes for 5 nm Colloidal Gold Conjugated Goat Anti-mouse IgG secondary antibody    Recommended Dilutions

EM: 1:20-50
IHC-P (paraffin-fixed section): 1:50-100
IHC-F (frozen-fixed section): 1:50-100
ICC: 1:50-100
Optimal working dilutions must be determined by end users.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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