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HRP Conjugated Goat Anti-mouse IgM Secondary Antibody

Scan QR to view Datasheet Catalog #    MBS176641 HRP Conjugated Goat Anti-mouse IgM secondary antibody
Unit / Price
0.25 mL  /  $160 +1 FREE 8GB USB
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 Product Name   

HRP Conjugated Goat Anti-mouse IgM, Secondary Antibody

★Popular Item★
 Also Known As   

HRP Conjugated Goat Anti-mouse IgM (u-chain specific) secondary antibody

 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Polyclonal
 Host    Goat
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 Specificity    This peroxidase conjugated antibody is specific for mouse IgM and shows no cross-reactivity with mouse IgA, IgG and other animal species IgM proteins.
 Form/Format    Concentrated, Liquid
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 Immunogen    Mouse IgM (whole molecule).
 Labeling Method    Goat anti-mouse IgM is conjugated to peroxidase by means of a method described by Wilson MB and Nakane PK.
(Reference: Wilson MB and Nakane PK. In Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, P215 (1978))
 Contents    0.25 mg of peroxidase conjugated specific antibody; 0.01 M PBS (pH7.4).
 Preparation of Diluent Buffer    Use 0.01 M TBS or 0.01 M PBS to dilute. See "Recommended Dilutions" below for details.
Preparation of 0.01 M TBS: Add 1.2g Tris, 8.5g NaCI; 4501-11 of purified acetic acid or 7001-11 of concentrated hydrochloric acid to 1000ml H20 and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation of 0.01 M PBS: Add 8.59 sod:um chloride, 1.4g Na2HP04 and 0.2g NaH2P04 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1 L.
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 Preparation and Storage    Store at 4 degree C for frequent use; at -20 degree C for at least one year. Avoid multiple freeze-thaw cycles.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of HRP Conjugated Goat Anti-mouse IgM secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for HRP Conjugated Goat Anti-mouse IgM secondary antibody

   Description: This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for mouse IgM.
 Applications Tested/Suitable for HRP Conjugated Goat Anti-mouse IgM secondary antibody   

Dot Blot (DB), Western Blot (WB), ELISA (EIA)

 Application Notes for HRP Conjugated Goat Anti-mouse IgM secondary antibody    Recommended Dilutions:

Dot blot (Enhanced chemiluminescent coloration): 1:2000-4000
WB (DAB coloration): 1:500-3000
WB (Enhanced chemiluminescent coloration): 1:3000-10000
Direct ELISA (TMB coloration): 1:20000-40000
Optimal working dilutions must be determined by end user.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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