View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List HRP Conjugated Goat Anti-mouse IgM Secondary Antibody Catalog # MBS176641 Unit / Price 0.25 mL / $200 +1 FREE 8GB USB 0.5 mL / $270 +1 FREE 8GB USB 5x0.5 mL / $1,035 +1 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name HRP Conjugated Goat Anti-mouse IgM, Secondary Antibody Popular Item Full Product Name HRP Conjugated Goat Anti-mouse IgM (u-chain specific) secondary antibody Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Polyclonal Isotype IgM Host Goat Species Reactivity Mouse Specificity This peroxidase conjugated antibody is specific for mouse IgM and shows no cross-reactivity with mouse IgG. Purity/Purification The antibody was purified from antiserum by immunoaffinity chromatography. Form/Format Concentrated, Liquid; 0.25 mg of peroxidase conjugated specific antibody, 0.01 M PBS (pH7.4), 50% glycerol. Concentration 1 mg/ml (lot specific) Immunogen Mouse IgM (whole molecular). Labeling Method Goat anti-mouse IgM is conjugated to peroxidase by means of a method described by Wilson MB and Nakane PK. (Reference: Wilson MB and Nakane PK. In Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, P215 (1978).) Preparation of Diluent Buffer Use 0.01M TBS or 0.01M PBS to dilute. See Recommended Dilutions below for details.- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L. Preparation and Storage Store at -20°C for one year from date of receipt. Avoid repeated freezing and thawing. ISO Certification Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory. Other Notes Small volumes of HRP Conjugated Goat Anti-mouse IgM secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for HRP Conjugated Goat Anti-mouse IgM secondary antibody This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for mouse IgM. Applications Tested/Suitable for HRP Conjugated Goat Anti-mouse IgM secondary antibody Western Blot (WB), ELISA Application Notes for HRP Conjugated Goat Anti-mouse IgM secondary antibody Recommended Dilutions:Western blot(ECL): 0.1-0.2ug/mlWestern blot(DAB): 0.7-3.3ug/mlDirect ELISA(TMB detection): 0.05-0.5ug/mlOptimal working dilutions must be determined by end users.Notes: Dilute with neutral PBS or TBS. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP