View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List HRP Conjugated Protein A Secondary Antibody Catalog # MBS176613 Unit / Price 0.5 mL / $235 +1 FREE 8GB USB 1 mL / $360 +1 FREE 8GB USB 5x1 mL / $1,435 +1 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name HRP Conjugated Protein A, Secondary Antibody Popular Item Full Product Name HRP Conjugated Protein A Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Host E. coli Recombinant Protein A Specificity This HRP conjugated Protein A. Human IgG1, IgG2 and IgG4 bind strongly, while IgG3 does not bind.There are also many instances in which monoclonal antibodies do not bind to Protein A, especially the majority of rat immunoglobulins and mouse IgG1. Form/Format Concentrated, Liquid ; 0.5 mg of HRP conjugated protein A, 0.01 M PBS (pH7.4), 50% glycerol. Concentration 1mg/ml (lot specific) Conjugation Peroxidase Suggested Working Concentration Western Blotting: 0.1-0.3ug/ml (ECL detection)Western Blotting: 0.7-3.3ug/ml (DAB detection)Direct ELISA: 0.1-0.5ug/ml (TMB detection)Optimal working dilutions muist be determined by end users. Preparation and Storage At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. ISO Certification Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory. Other Notes Small volumes of HRP Conjugated Protein A secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Applications Tested/Suitable for HRP Conjugated Protein A secondary antibody Western Blot (WB), ELISA (EIA) Application Notes for HRP Conjugated Protein A secondary antibody Dilute with neutral PBS or TBS.Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP