Specificity
Mouse Ly-6G, Mr 21-25 kDa
Ly-6G, formerly known as the myeloid differentiation antigen Gr-1, is a GPI-anchored protein.1 In the bone marrow, the level of antigen expression is directly correlated with granulocyte differentiation and maturation. It is also transiently expressed on monocytes in the bone marrow.2 In the periphery, Ly-6G is expressed predominantly on neutrophils.3, 4
Characterization
To ensure lot-to-lot consistency, each batch of product is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative flow data are included in this product insert.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg of 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD), R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5), R-phycoerythrin-Cyanine 7 (R-PE-CY 7) and Allophycocyanin -Cyanine 7 (APC-CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
Other Notes
Small volumes of Ly-6G (GR-1) secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product References and Citations for
Ly-6G (GR-1) secondary antibody
1. Fleming, T.J., M.L. Fleming and T. R. Malek. 1993. J. Immunol. 151:2399.
2. Hestdal, K., F.W. Ruscetti, J.N. Ihle, et al. 1991. J. Immunol. 147:22.
3. Lewinsohn, D.M., R.F. Bargatze, and E.C. Butcher. 1987. J. Immunol. 138:4313.
4. Lagasse, E., and I.L. Weissman. 1996. J. Immunol. Meth. 197:139.
6. Jutila, M.A., F.G.M. Kroese, K.L. Jutila, A.M. Stall, et al. 1988. Eur. J. Immunol. 18:1819.
7. Brummer, E., A.M. Sugar, and D.A. Stevens. 1984. J. Leukocyte Biol. 36:505.
8. Stoppacciaro, A., C. Melani, M. Parenza, et al. 1993. J. Exp. Med. 178:151.
9. Tumpey, T.M., S.-H. Chen, J.E. Oakes, and R.N. Lausch. 1996. J. Virol. 70:898.
10. Conlan, J.W., and.J. North. 1994. J. Exp. Med. 179:259.
11. Czuprynski, C.J., J.F. Brown, N. Maroushek, R.D. Wagner, and H. Steinberg. 1994. J. Immunol. 152:1836.
12. Rakhmilevich, A.L. 1995. J. Leukoc. Biol. 57:827.
Precautions
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