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Rabbit anti Goat IgM (Fc specific) Secondary Antibody

Scan QR to view Datasheet Catalog #    MBS571819 Rabbit anti Goat IgM (Fc specific) secondary antibody
Unit / Price
1 mL  /  $570 +1 FREE 8GB USB
 
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 Product Name   

Rabbit anti Goat IgM (Fc specific), Secondary Antibody

★Popular Item★
 Also Known As   

Rabbit anti Goat IgM (Fc specific), conjugated with Biotin

 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Goat
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 Specificity    Biotin-conjugated IgG fraction of polyclonal Rabbit antiSerum to Goat IgM, Fc specific.
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 Source    Purified normal IgM isolated from pooled goat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure
 Product    Biotin-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.
 Conjugation    Biotin
 Product Type    Secondary Antibodies
 Reactivity Note    Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, however a strong reaction with Sheep IgM has been observed.
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 Preparation and Storage    The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate
 Other Notes    Small volumes of Rabbit anti Goat IgM (Fc specific) secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for Rabbit anti Goat IgM (Fc specific) secondary antibody

   Background:
The reactivity of the antiserum is directed to the Fc subunit of the IgM molecule which expresses strict isotypic (class) specificity. It does not react with any non-Ig protein in goat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of goat origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in goat serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
 Product Categories/Family for Rabbit anti Goat IgM (Fc specific) secondary antibody    Conjugated Secondary Antibody
 Applications Tested/Suitable for Rabbit anti Goat IgM (Fc specific) secondary antibody   

ELISA, Immunocytochemistry, Immunohistochemistry (paraffin), Dot blot, Immunoblotting.

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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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